Changes in Gene Expression of Commercial Baker's Yeast during an Air-Drying Process that Simulates Dried Yeast Production

Nakamura, Toshihide; Mizukami-Murata, Satomi; Andõ, Akira; Murata, Yoshinori; Takagi, Hiroshi; Shima, Jun

doi:10.1263/jbb.106.405

Cited by 18 publications

(10 citation statements)

References 16 publications

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“…The experimental procedures used for the RNA extraction and DNA microarray analysis have been described previously (Nakamura et al [2008]). The total RNA was quantified using a Bioanalyzer 2100 with an RNA 6000 Nano LabChip kit (Agilent Technologies, Palo Alto, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Identification of a gene, FMP21, whose expression levels are involved in thermotolerance in Saccharomyces cerevisiae

et al. 2014

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Elucidation of the mechanism of high temperature tolerance in yeasts is important for the molecular breeding of high temperature-tolerant yeasts that can be used in bioethanol production. We identified genes whose expression is correlated with the degree of thermotolerance in Saccharomyces cerevisiae by DNA microarray analysis. Gene expression profiles of three S. cerevisiae strains showing different levels of thermotolerance were compared, and we chose three of them as candidate genes. Among these genes, FMP21 was investigated as a thermotolerance-related gene in S. cerevisiae by comparing the growth at high temperature with the gene expression in eight strains. The expression ratio of FMP21 at 37°C was correlated with the doubling time ratio at a coefficient of determination of 0.787. The potential involvement of the Fmp21 in the thermotolerance of yeasts was evaluated. The FMP21 deletion variant showed a decreased respiratory growth rate and increased thermosensitivity. Furthermore, the overexpression of FMP21 improved thermotolerance in yeasts. In conclusion, the function of Fmp21 is important for thermotolerance in yeasts.

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Section: Methodsmentioning

confidence: 99%

Identification of a gene, FMP21, whose expression levels are involved in thermotolerance in Saccharomyces cerevisiae

et al. 2014

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“…Yeast, 29 probiotics, 30 proteins, 31,32 antibodies, 33 enzymes, 34 platelets, 35 fish, 36 encountered in developing an efficient drying process. Depending on the energy source and the configuration of the drying system, the parameters to be optimized will differ, as will be described below.…”

Section: Convective Dryingmentioning

confidence: 99%

Next Generation Drying Technologies for Pharmaceutical Applications

Walters

Bhatnagar

Tchessalov

et al. 2014

Journal of Pharmaceutical Sciences

179

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“…In addition to analyses of cell‐protective molecules, the identification of novel genes which determine the tolerance to baking‐associated stress were attempted. Our group attempted to find the genes for stress tolerance by two different approaches, namely phenomics and functional genomics, under freeze–thaw, high‐sugar‐concentration and air‐drying stress conditions [6,7,31,86–89]. The data for phenomics and functional genomics under baking‐associated stress conditions are available at our web site (http://nfri.naro.affrc.go.jp/english/Useful/yeast/index.pdf).…”

Section: Phenomics Approach To the Identification Of Genes Required Fmentioning

confidence: 99%

“…Singh et al analysed the transcriptional response of a laboratory yeast strain during dehydration and rehydration processes under minimal‐glucose conditions [110]. To gain insight into the physiology of yeast cells during the commercial production of dried yeast, we obtained gene‐expression profiles under air‐drying stress [86]. In a different way from Singh et al [110], we analysed gene expression in commercial baker's yeast under conditions that simulate conditions used in dried‐yeast production.…”

Section: Functional‐genomics Approach To the Identification Of Genes mentioning

confidence: 99%

Stress‐tolerance of baker's‐yeast (Saccharomyces cerevisiae) cells: stress‐protective molecules and genes involved in stress tolerance

Shima

Takagi

2009

Biotech and App Biochem

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During the fermentation of dough and the production of baker's yeast (Saccharomyces cerevisiae), cells are exposed to numerous environmental stresses (baking-associated stresses) such as freeze-thaw, high sugar concentrations, air-drying and oxidative stresses. Cellular macromolecules, including proteins, nucleic acids and membranes, are seriously damaged under stress conditions, leading to the inhibition of cell growth, cell viability and fermentation. To avoid lethal damage, yeast cells need to acquire a variety of stress-tolerant mechanisms, for example the induction of stress proteins, the accumulation of stress protectants, changes in membrane composition and repression of translation, and by regulating the corresponding gene expression via stress-triggered signal-transduction pathways. Trehalose and proline are considered to be critical stress protectants, as is glycerol. It is known that these molecules are effective for providing protection against various types of environmental stresses. Modifications of the metabolic pathways of trehalose and proline by self-cloning methods have significantly increased tolerance to baking-associated stresses. To clarify which genes are required for stress tolerance, both a comprehensive phenomics analysis and a functional genomics analysis were carried out under stress conditions that simulated those occurring during the commercial baking process. These analyses indicated that many genes are involved in stress tolerance in yeast. In particular, it was suggested that vacuolar H+-ATPase plays important roles in yeast cells under stress conditions.

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Changes in Gene Expression of Commercial Baker's Yeast during an Air-Drying Process that Simulates Dried Yeast Production

Cited by 18 publications

References 16 publications

Identification of a gene, FMP21, whose expression levels are involved in thermotolerance in Saccharomyces cerevisiae

Identification of a gene, FMP21, whose expression levels are involved in thermotolerance in Saccharomyces cerevisiae

Next Generation Drying Technologies for Pharmaceutical Applications

Stress‐tolerance of baker's‐yeast (Saccharomyces cerevisiae) cells: stress‐protective molecules and genes involved in stress tolerance

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