Identification of a gene, FMP21, whose expression levels are involved in thermotolerance in Saccharomyces cerevisiae

Abstract

Elucidation of the mechanism of high temperature tolerance in yeasts is important for the molecular breeding of high temperature-tolerant yeasts that can be used in bioethanol production. We identified genes whose expression is correlated with the degree of thermotolerance in Saccharomyces cerevisiae by DNA microarray analysis. Gene expression profiles of three S. cerevisiae strains showing different levels of thermotolerance were compared, and we chose three of them as candidate genes. Among these genes, FMP21 was investigated as a thermotolerance-related gene in S. cerevisiae by comparing the growth at high temperature with the gene expression in eight strains. The expression ratio of FMP21 at 37°C was correlated with the doubling time ratio at a coefficient of determination of 0.787. The potential involvement of the Fmp21 in the thermotolerance of yeasts was evaluated. The FMP21 deletion variant showed a decreased respiratory growth rate and increased thermosensitivity. Furthermore, the overexpression of FMP21 improved thermotolerance in yeasts. In conclusion, the function of Fmp21 is important for thermotolerance in yeasts.

Comprehensive understanding of Saccharomyces cerevisiae phenotypes with whole‐cell model WM_S288C

“…The predicted AA concentrations were linearly correlated (0.79 PCC, p = 4.1 × 10 −5 ) with measured AA composition (Figure 3a). At 30°C, simulated messenger RNA (0.99 PCC, p = 4.5 × 10 –18 ) and protein abundances (0.32 PCC, p = 0.085) were similar to experimental values (Christiano, Nagaraj, Frohlich, & Walther, 2014; Nakamura, Yamamoto, Saito, Ando, & Shima, 2014; Figure 3b,c). Taken together, we believe that the WM_S288C model can recapitulate experimental data across multiple biological functions and scales.…”

Selection and Development of Stress-tolerant Yeasts for Bioethanol Production

“…We searched for genes whose expression levels were correlated with the degree of high temperature tolerance. After validation by real-time polymerase chain reaction (PCR), one candidate gene-SDH8 (formerly FMP21)-was identified (Nakamura et al 2014).…”

Genetic basis of variation in heat and ethanol tolerance in Saccharomyces cerevisiae

“…Various strategies have been used to understand the genetic basis of ethanol and thermal tolerance in yeast. Screens of the yeast deletion collection have uncovered several hundred genes involved in ethanol and temperature tolerance that cover a broad range of functional categories (Fujita et al 2006;Van Voorst et al 2006;Yoshikawa et al 2009;Auesukaree et al 2009;Ruiz-Roig et al 2010;Nakamura et al 2014). Genes have also been identified from experimental evolution with selection for high ethanol (Voordeckers et al 2015) or temperature (Caspeta et al 2014;Huang et al 2018) tolerance.…”