Changes in Gene Expression in Human Epithelial and Mast Cells in Response to Vesicles from Klebsiella pneumonia ATCC 13883

Abstract

We investigated alterations in the expression of immune-related genes in epithelial cells and mast cells treated with outer membrane vesicles (OMVs) derived from Klebsiella pneumoniae (KpOMVs). Previous studies have shown that OMVs contain substances that enable their delivery to host cells and induce an immune response. Our results indicate an increase in expression of genes such as IL-8, IL-1b, MIP-1a, HMOX1, HSPA1A, and IL-24 in epithelial cells and mast cells treated with KpOMVs. The pathogenicity of KpOMVs was confirmed by measuring the changes in the expression of these immune-related genes.

Roles of bacterial extracellular vesicles in systemic diseases

“…MVs produced by S. aureus also fuse in a cholesterol-dependent manner with the plasma membrane of host cells, causing the delivery of α-hemolysin (HIa), which can trigger apoptosis in T-lymphocytes ( Thay et al, 2013 ). Klebsiella pneumoniae OMVs increased the proinflammatory cytokines IL-1β, IL-8 and TNF-α in human epithelial cells, mast cells and macrophages ( You et al, 2019 ). Likewise, OMVs secreted by respiratory pathogens induced a strong proinflammatory response in immature THP-1 macrophages ( Volgers et al, 2017 ).…”

The Contribution of Membrane Vesicles to Bacterial Pathogenicity in Cystic Fibrosis Infections and Healthcare Associated Pneumonia

“…MVs originated from K. pneumoniae ATCC 13883 likewise induced changes in the expression of immune-related genes in epithelial cells. The expression of genes encoding for IL-8, IL-1b, MIP-1α, HMOX1, HSPA1A, and IL-24, was increased after treatment of these cells with K. pneumoniae MVs (Lee et al, 2012;You et al, 2019). Further, several Gramnegative bacteria bind to epithelial cells through lipid rafts and deliver peptidoglycan-containing MVs to the intracellular sensor NOD1 to promote inflammation (Kaparakis et al, 2010).…”