Roles of bacterial extracellular vesicles in systemic diseases

Wang, Yanzhen; Luo, Xinghong; Xiang, Xiaozhen; Hao, Chunbo; Ma, Dandan

doi:10.3389/fmicb.2023.1258860

Cited by 4 publications

(2 citation statements)

References 172 publications

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“…During bacterial colonization of tissues, either as microbiota or infection, BEVs may be shed directly by pathogens or EVs by pathogen-infected cells [215] . BEVs carry microbial-or pathogen-associated molecular patterns (MAMPs/PAMPs) that can be recognized by pattern recognition receptors (PRRs) on the surface and in the cytoplasm of immune and non-immune cells [14,216,217] [Figure 3].…”

Section: Bev-induced Inflammatory Responsesmentioning

confidence: 99%

Cargo exchange between human and bacterial extracellular vesicles in gestational tissues: a new paradigm in communication and immune development

Amabebe,

Kumar,

Tatiparthy

et al. 2024

Extracell Vesicles Circ Nucleic Acids

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Host-bacteria and bacteria-bacteria interactions can be facilitated by extracellular vesicles (EVs) secreted by both human and bacterial cells. Human and bacterial EVs (BEVs) propagate and transfer immunogenic cargos that may elicit immune responses in nearby or distant recipient cells/tissues. Hence, direct colonization of tissues by bacterial cells is not required for immunogenic stimulation. This phenomenon is important in the feto-maternal interface, where optimum tolerance between the mother and fetus is required for a successful pregnancy. Though the intrauterine cavity is widely considered sterile, BEVs from diverse sources have been identified in the placenta and amniotic cavity. These BEVs can be internalized by human cells, which may help them evade host immune surveillance. Though it appears logical, whether bacterial cells internalize human EVs or human EV cargo is yet to be determined. However, the presence of BEVs in placental tissues or amniotic cavity is believed to trigger a low-grade immune response that primes the fetal immune system for ex-utero survival, but is insufficient to disrupt the progression of pregnancy or cause immune intolerance required for adverse pregnancy events. Nevertheless, the exchange of bioactive cargos between human and BEVs, and the mechanical underpinnings and health implications of such interactions, especially during pregnancy, are still understudied. Therefore, while focusing on the feto-maternal interface, we discussed how human cells take up BEVs and whether bacterial cells take up human EVs or their cargo, the exchange of cargos between human and BEVs, host cell (feto-maternal) inflammatory responses to BEV immunogenic stimulation, and associations of these interactions with fetal immune priming and adverse reproductive outcomes such as preeclampsia and preterm birth.

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Section: Bev-induced Inflammatory Responsesmentioning

confidence: 99%

Cargo exchange between human and bacterial extracellular vesicles in gestational tissues: a new paradigm in communication and immune development

Amabebe,

Kumar,

Tatiparthy

et al. 2024

Extracell Vesicles Circ Nucleic Acids

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“…The internal cargo of BEVs is protected from degradation in the extracellular environment by enzymes. Moreover, BEVs contain pathogen-associated molecular patterns and can initiate inflammatory signaling pathways in host cells [7]. They may therefore play an essential role in the pathogenesis of various diseases.…”

Section: Introductionmentioning

confidence: 99%

Rapid Biodistribution of Fluorescent Outer-Membrane Vesicles from the Intestine to Distant Organs via the Blood in Mice

Schaack,

Mercier,

Katby

et al. 2024

IJMS

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A cell’s ability to secrete extracellular vesicles (EVs) for communication is present in all three domains of life. Notably, Gram-negative bacteria produce a specific type of EVs called outer membrane vesicles (OMVs). We previously observed the presence of OMVs in human blood, which could represent a means of communication from the microbiota to the host. Here, in order to investigate the possible translocation of OMVs from the intestine to other organs, the mouse was used as an animal model after OMVs administration. To achieve this, we first optimized the signal of OMVs containing the fluorescent protein miRFP713 associated with the outer membrane anchoring peptide OmpA by adding biliverdin, a fluorescence cofactor, to the cultures. The miRFP713-expressing OMVs produced in E. coli REL606 strain were then characterized according to their diameter and protein composition. Native- and miRFP713-expressing OMVs were found to produce homogenous populations of vesicles. Finally, in vivo and ex vivo fluorescence imaging was used to monitor the distribution of miRFP713-OMVs in mice in various organs whether by intravenous injection or oral gavage. The relative stability of the fluorescence signals up to 3 days post-injection/gavage paves the way to future studies investigating the OMV-based communication established between the different microbiotas and their host.

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Unveiling clinical applications of bacterial extracellular vesicles as natural nanomaterials in disease diagnosis and therapeutics

Liu,

Yazdani,

Moran

et al. 2024

Acta Biomaterialia

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Roles of bacterial extracellular vesicles in systemic diseases

Cited by 4 publications

References 172 publications

Cargo exchange between human and bacterial extracellular vesicles in gestational tissues: a new paradigm in communication and immune development

Cargo exchange between human and bacterial extracellular vesicles in gestational tissues: a new paradigm in communication and immune development

Rapid Biodistribution of Fluorescent Outer-Membrane Vesicles from the Intestine to Distant Organs via the Blood in Mice

Unveiling clinical applications of bacterial extracellular vesicles as natural nanomaterials in disease diagnosis and therapeutics

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