Changes in fatty acid composition in Pseudomonas putida and Pseudomonas stutzeri during naphthalene degradation

Mrozik, Agnieszka; Labuzek, S.; Piotrowska‐Seget, Zofia

doi:10.1016/j.micres.2004.11.001

Cited by 48 publications

(40 citation statements)

References 35 publications

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“…In the presence of catechol, phenol or these mixtures it showed the value 6.5, 5.68 and 6.38, respectively ( Table 2). The similar correlation between an increase in degree of membrane saturation and tolerance towards the toxic aromatic compounds was earlier observed in studies using Pseudomonas putida P8 (Heipieper et al, 1992), P. putida and P. stutzeri (Mrozik et al, 2005), Rhodococcus sp. 33 (Gutierrez et al, 1999) and Ralstonia eutropha H850 (Kim et al, 2001).…”

Section: Fatty Acid Composition Analysissupporting

confidence: 83%

Cellular fatty acid patterns inPseudomonas sp. CF600 during catechol and phenol degradation in media supplemented with glucose as an additional carbon source

2006

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Fatty acid composition in Pseudomonas sp. CF600 during degradation of catechol and phenol individually and their mixture was investigated. Moreover, the influence of glucose as an additional, easily degradable carbon source on fatty acid profiling in bacteria grown on these aromatic substrates was studied. Both catechol and phenol treatments caused in bacterial cells crucial changes in the distribution of tested groups of fatty acids. The major changes included the increase of fatty acid saturation, decrease in the percentage of cyclopropane fatty acid 17:0 cy and the appearance of branched and hydroxy fatty acids. Under catechol, phenol and their mixture exposure saturated/unsaturated ratio showed the value 6.5, 5.68 and 6.38 whereas in control cells this ratio reached the value 3.05. As a response to aromatic compounds bacteria formed fatty acids that were not detected in control cells growing on glucose. It has been demonstrated that the supplementation of cultured media containing single aromatic substrates or/and their mixture with glucose resulted in changes in degradation rates of catechol and phenol. It seemed that glucose influenced some metabolic pathways responsible for the assimilation of aromatic compounds. The incubation of cells in the presence of aromatic compounds and glucose rapidly led to alterations of whole-cell derived fatty acid composition. The most important changes were associated with saturation level of fatty acids and cyclopropane fatty acid contents.

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Section: Fatty Acid Composition Analysissupporting

confidence: 83%

Cellular fatty acid patterns inPseudomonas sp. CF600 during catechol and phenol degradation in media supplemented with glucose as an additional carbon source

2006

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“…Changes of cellular fatty acid and/or mycolic acid profile have been reported from several PAH-degrading bacteria (Mrozik et al 2005;Tsitko et al 1999;Wick et al 2002Wick et al , 2003. These adaptations usually provide bacterial cells to change their membrane fluidity with concomitant regulation of harmful chemicals flux.…”

Section: Discussionmentioning

confidence: 99%

Comparative metabolomic analysis of Sinorhizobium sp. C4 during the degradation of phenanthrene

Keum

Seo

et al. 2008

Appl Microbiol Biotechnol

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Comparative metabolic responses of Sinorhizobium sp. C4 were investigated. Comprehensive metabolites profiles, including polar metabolites, fatty acids, and polyhydroxyalkanoates were evaluated through untargeted metabolome analyses. Intracellular metabolomes during the degradation of phenanthrene were compared with those from natural carbon sources. Principal component analysis showed a clear separation of metabolomes of phenanthrene degradation from other carbon sources. Shift to more hydrophobic fatty acid was observed from the analysis of fatty acid methyl ester. Polyhydroxyalkanoate from strain C4 was composed mainly with 3-hydroxybutyric acid and small amount of 3-hydroxypentanoic acid, while the monomeric composition was independent on carbon sources. However, the amount of polyhydroxyalkanoates during degradation of phenanthrene was 50-210% less than those from other carbon sources. Among 207 gas chromatography-mass spectrometry peaks from the polar metabolite fraction, 60% of the peaks were identified and compared. Several intermediates in tricarboxylic acid cycles and glycolysis were increased during phenanthrene degradation. Accumulation of trehalose was also evident in the phenanthrene-treated bacterium. Some amino acid, including branched amino acids, glycine, homoserine, and valine, were also increased, while more than 70% of identified metabolites were decreased during the phenanthrene metabolism. Accumulation of sulfur amino acids and nicotinic acid suggested the possible oxidative stress conditions during phenanthrene metabolism.

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“…Mrozik et al describe the changes in fatty acid composition in strains of P. putida and P. stutzeri during naphthalene degradation (232,233). The reaction of both strains to the addition of naphthalene was an increase in the saturated/unsaturated ratio and alterations in the percentage of hydroxy, cyclopropane, and branched fatty acids.…”

Section: Chemical Characterization and Chemotaxonomymentioning

confidence: 99%

Biology of Pseudomonas stutzeri

Lalucat

Bennasar

Bosch

et al. 2006

Microbiol Mol Biol Rev

546

418

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SUMMARY Pseudomonas stutzeri is a nonfluorescent denitrifying bacterium widely distributed in the environment, and it has also been isolated as an opportunistic pathogen from humans. Over the past 15 years, much progress has been made in elucidating the taxonomy of this diverse taxonomical group, demonstrating the clonality of its populations. The species has received much attention because of its particular metabolic properties: it has been proposed as a model organism for denitrification studies; many strains have natural transformation properties, making it relevant for study of the transfer of genes in the environment; several strains are able to fix dinitrogen; and others participate in the degradation of pollutants or interact with toxic metals. This review considers the history of the discovery, nomenclatural changes, and early studies, together with the relevant biological and ecological properties, of P. stutzeri.

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Changes in fatty acid composition in Pseudomonas putida and Pseudomonas stutzeri during naphthalene degradation

Cited by 48 publications

References 35 publications

Cellular fatty acid patterns inPseudomonas sp. CF600 during catechol and phenol degradation in media supplemented with glucose as an additional carbon source

Cellular fatty acid patterns inPseudomonas sp. CF600 during catechol and phenol degradation in media supplemented with glucose as an additional carbon source

Comparative metabolomic analysis of Sinorhizobium sp. C4 during the degradation of phenanthrene

Biology of Pseudomonas stutzeri

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