Changes in DNA and microtubules during loss and re-establishment of desiccation tolerance in germinating Medicago truncatula seeds

Faria, José Márcio Rocha; Buitink, Julia; Lammeren, A.A.M. van; Hilhorst, Henk W. M.

doi:10.1093/jxb/eri210

Cited by 97 publications

(104 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Since these two protein families have been associated with desiccation tolerance (Delseny et al, 1994), it is possible that both osmotic and heat stress can also enhance desiccation tolerance in sensitive seeds. Osmotic stress (PEG) is also capable of re-establishing desiccation tolerance in germinated seeds of cucumber (Bruggink and van der Toorn, 1995;Leprince et al, 2000) and Medicago truncatula (Buitink et al, 2003;Faria et al, 2005). Intermittent heat-shock (HS) treatment has been used to improve low-temperature fruit storage of recalcitrant species, such as mango and avocado (Marcellin, 1992).…”

Section: Introductionmentioning

confidence: 99%

Physiological and cytological aspects of Inga vera subsp. affinis embryos during storage

Faria

Davide

Silva

et al. 2006

Braz. J. Plant Physiol.

View full text Add to dashboard Cite

The remarkably short storability of recalcitrant seeds has prevented their inclusion in programs of ex situ conservation. The causes of their desiccation sensitivity and rapid decline in viability during storage are not fully elucidated yet. In this study the highly recalcitrant, fully viable embryos of the tree species Inga vera subsp. affinis were stored under various conditions and analyzed physiologically and cytologically at intervals, in order to obtain more insights in their loss of viability during storage. Embryos stored fully hydrated at 5 o C fully lost viability in 18 d. Sealed storage of partially dehydrated embryos slightly prolonged their viability, with 95% germination being attained after 14 d of storage. However, after 30 d of storage, viability was completely lost. Storage of hydrated embryos in a solution of polyethylene glycol (PEG) at -1.7 MPa water potential was capable of maintaining high germinability until 30 d of storage. When added to the PEG solution, abscisic acid showed a strong temperature-dependent interaction, with a positive effect on the longevity of embryos stored at 20 o C and a negative effect on embryos stored at 5 o C. In any case, embryo viability could be maintained no longer than 50 d. Embryos from seeds collected from the same trees in the following year showed better storability and still attained 45% germination after 62 d of storage. Analysis of cellular alterations during storage and viability loss of the embryos showed disappearance of starch granules and various damages to the cells, such as cell wall folding and cytoplasm fragmentation. Key-words: abscisic acid, polyethylene glycol, recalcitrant seeds, seed conservationAspectos fisiológicos e citológicos de embriões de Inga vera subsp. affinis durante o armazenamento: A capacidade reduzida de armazenamento apresentada pelas sementes recalcitrantes tem impedido sua inclusão em programas de conservação ex situ. As causas da rápida queda da viabilidade durante o armazenamento ainda não estão totalmente elucidadas. Visando, portanto, a um melhor entendimento desse comportamento, embriões de sementes recalcitrantes de Inga vera subsp. affinis foram armazenados sob várias condições e avaliados periodicamente com relação a aspectos fisiológicos e citológicos. Embriões armazenados sem secagem, a 5 o C, perderam totalmente a viabilidade em 18 dias. Embriões parcialmente desidratados apresentaram 95% de germinação após 14 dias de armazenamento em sacos plásticos selados. Entretanto, após 30 dias de armazenamento, a viabilidade foi totalmente perdida. O armazenamento de embriões sem secagem em solução de polietileno glicol (PEG) a um potencial hídrico de -1,7 MPa foi capaz de manter uma alta capacidade de germinação até 30 dias de armazenamento. Quando adicionado à solução de PEG, o ácido abscísico mostrou uma forte interação com a temperatura de armazenamento, com um efeito positivo na longevidade dos embriões armazenados a 20 o C, porém um efeito negativo nos embriões armazenados a 5 o C. No entanto, em nenhuma condição...

show abstract

Section: Introductionmentioning

confidence: 99%

Physiological and cytological aspects of Inga vera subsp. affinis embryos during storage

Faria

Davide

Silva

et al. 2006

Braz. J. Plant Physiol.

View full text Add to dashboard Cite

show abstract

“…This suggests that the majority of cells were retained in G 1 phase, while a small number were in G 2 phase of the cellular cycle. This is a prevalent characteristic in orthodox seeds (Bino et al 1993, Castro et al 2000, Vázquez-Ramos and Sánchez 2003, Gendreau et al 2008, although there are reports of dispersed orthodox seeds with high 4C DNA contents (approximately 40%) (Faria et al 2005, Guimarães et al 2011. High 2C DNA contents have also been observed in the mature seeds of intermediate and recalcitrant species, such as Castanea sativa (Bino et al 1993), Inga vera (Faria et al 2004) and Coffea arabica , indicating that the relationship between tolerance to desiccation and events inherent to the cellular cycle, is weak.…”

Section: Discussionmentioning

confidence: 99%

“…In some species, however, this structure remains tolerant, only becoming sensitive with an increase in length. In the seeds of tomato (Lin et al 1998) and Medicago truncatula (Faria et al 2005), for example, loss of tolerance to desiccation occurs at the point at which the primary roots reach 2 mm in length. In the present study, no seed with 1 mm radicle survived after the drying process was performed.…”

Section: Discussionmentioning

confidence: 99%

“…However, in the seeds of A. pavonina, loss of tolerance to desiccation was initially observed after 48 h of imbibition; i.e., the development of sensitivity to desiccation preceded the activation of cellular division. Although it has been proposed that cells with a 4C DNA content are more sensitive to stress conditions because they are more vulnerable to factors inducing mutation Barsy 1985, Sliwinska 2009), cells with a 2C DNA content have been shown to be sensitive to desiccation in the seeds of A. pavonina, Medicago truncatula (Faria et al 2005) and Peltophorum dubium (Guimarães et al 2011).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Physiological and biochemical changes during the loss of desiccation tolerance in germinating Adenanthera pavonina L. seeds

Soares

Dias

Faria

et al. 2015

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

We investigated the loss of desiccation tolerance (DT) in Adenanthera pavonina seeds during germination. Seeds were subjected to imbibition for 0, 24, 36, 48, 60 and 81 h, then dried to their initial moisture content (13%), rehydrated and evaluated for survival (resumption of growth and development of normal seedlings) and membrane system integrity (electrolyte leakage). Embryonic axes of seeds subjected only to imbibition during the same early time periods were used to investigate the electrophoretic patterns of heat-stable proteins and the relative nuclear DNA content. In A. pavonina seeds, DT remained unchanged until 36 h of imbibition (resulting in germination and 82% normal seedlings), after which it was progressively lost, and seeds with a protruded radicle length of 1 mm did not withstand dehydration. The loss of desiccation tolerance could not be related to either membrane damage caused by drying or the resumption of the cell cycle during germination. However, the decrease in heat-stable protein contents observed throughout germination may be related to the loss of DT in A. pavonina seeds.

show abstract

“…Drought stress was imposed with PEG (6000), -1.7 MPa, at 5 °C in the dark for three days (Buitink et al, 2003;Faria et. al.…”

Section: Treatments For Cross-tolerance Inductionmentioning

confidence: 99%

Cross-Tolerance Mechanism Induction in Melon Seeds by Priming Prior Drying

et al. 2015

View full text Add to dashboard Cite

The loss of benefits after re-drying is one of the drawbacks of the seed priming technique. Different types of stresses have been used before re-drying to preserve the priming benefits. This process may be seen as promoting cross tolerance to increase the defense mechanisms that prevent loss of viability in seeds primed after drying. We tested the effect of some stresses to induce cross-tolerance and different drying conditions with the aim of maintaining priming benefits in melon seeds. The seeds were primed in an aerated KNO 3 solution (0.35M), -1.7MPa, 25 °C, in the dark for six days. The primed seeds were then submitted to slow drying, fast drying, cold shock + slow drying, cold shock + fast drying, heat shock + slow drying, heat shock + fast drying, PEG + slow drying, PEG + fast drying, ABA + slow drying, ABA + fast drying and no drying (planted directly after priming). We evaluated antioxidant enzyme activities (SOD, CAT and APX), germinability, mean time of germination (MTG) and mean rate of germination (MRG). A completely randomized design was used with three repetitions of 50 seeds in each treatment. Data were analyzed by ANOVA and means were compared by the Scott-Knott test (p ≤ 0.05). ABA increased SOD activity after drying and CAT activity was reduced by priming. APX activity was not observed. The stress submission prior to re-drying improved the MRG and reduced MTG. Therefore, the induction of the cross-tolerance mechanism could be effective to maintain priming benefits in melon seeds.Index terms: PEG; priming; dry seeds; antioxidant system. RESUMOA perda dos benefícios do condicionamento, após a ressecagem, é um dos problemas do condicionamento. Para conservar os benefícios, têm sido utilizados diferentes estresses antes da secagem. Esse processo poderia agir como tolerância cruzada, aumentando os mecanismos de defesa, prevenindo a perda de viabilidade em sementes condicionadas após a secagem. Assim, testou-se o efeito de estresses, na tentativa de induzir tolerância cruzada, após diferentes secagens, mantendo os benefícios do condicionamento em sementes de melão. Dessa forma, as sementes foram condicionadas em solução aerada de KNO 3 (0,35M), -1,7 MPa, 25 °C, no escuro, por seis dias. Depois disso, foram submetidas à secagem lenta, rápida, choque de frio + secagem lenta e rápida, choque de calor + secagem lenta e rápida, PEG + secagem lenta e rápida, ABA + secagem lenta e rápida e sementes sem secagem. As sementes não condicionadas foram o controle. Foi avaliada a atividade das enzimas SOD, CAT e APX, germinabilidade, tempo médio de germinação e taxa média de germinação. O delineamento experimental utilizado foi inteiramente casualisado (DIC) com três repetições, contendo 50 sementes para cada tratamento. Para a análise estatística dos dados, foi utilizado ANOVA e as médias comparadas pelo teste de Scott-Knott (p ≤ 0,05). A aplicação de ABA aumentou a atividade da SOD após a secagem e o condicionamento reduziu a atividade da CAT. O estresse inicial, antes da secagem, aumentou-a e reduziu o...

show abstract

Changes in DNA and microtubules during loss and re-establishment of desiccation tolerance in germinating Medicago truncatula seeds

Cited by 97 publications

References 47 publications

Physiological and cytological aspects of Inga vera subsp. affinis embryos during storage

Physiological and cytological aspects of Inga vera subsp. affinis embryos during storage

Physiological and biochemical changes during the loss of desiccation tolerance in germinating Adenanthera pavonina L. seeds

Cross-Tolerance Mechanism Induction in Melon Seeds by Priming Prior Drying

Contact Info

Product

Resources

About