Changes in distribution of Ia antigen on epithelium of the jejunum and ileum in rats infected with Nippostrongylus brasiliensis

Masson, Stephen; Perdue, Mary H.

doi:10.1016/0090-1229(90)90024-k

Cited by 19 publications

(3 citation statements)

References 22 publications

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“…These results are consistent with the report that at least some species of macromolecules can be transported across the normal mature gut by a pinocytotic processes (Walker and Isselbacher, 1974). Since villus epithelial cells express MHC class II antigens and its expression was enhanced after N. brasiliensis infection (Mayrhofer and Spargo, 1990;Masson and Perdue, 1990), it is possible that some of the processed antigens are directly presented from intestinal absorptive cells to lymphocytes.…”

Section: Methodssupporting

confidence: 90%

Mechanisms of Parasite Antigen Uptake from the Intestine of Rats Infected with the Nematode Nippostrongylus brasiliensis.

Yamada

Uchikawa

Matsuda

et al. 2001

Jap. J. Trop. Med. Hyg.

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The mechanisms of antigen-uptake at the host mucosal barrier during infection with lumen-dwelling intestinal parasites have not been well elucidated. In this study, we investigated immunolocalization of parasite excretory/secretory (ES) products in the small intestinal epithelium of rats infected with Nippostrongylus brasiliensis. Immunoelectronmicroscopy revealed intense ES immunoreactivity in the absorptive cell cytoplasm in association with lysosome-associated membrane glycoprotein (LAMP-1), and in the paracellular spaces. These results indicated that nematode antigen could be absorbed by two pathways: absorption by endocytosis and transport to lysosomes, and absorption through the paracellular pathway, suggesting altered mucosal barrier function.

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Section: Methodssupporting

confidence: 90%

Mechanisms of Parasite Antigen Uptake from the Intestine of Rats Infected with the Nematode Nippostrongylus brasiliensis.

Yamada

Uchikawa

Matsuda

et al. 2001

Jap. J. Trop. Med. Hyg.

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“…These investigators interpreted these findings to indicate that other cells, possibly epithelial cells, rather than gut leucocytes were mainly responsible for the amount of class II chain transcripts detected in the hindgut. In mammals, epithelial cells in a number of di#erent organs including the intestine carry Mhc class II antigens (Mayrhofer et al, 1983;Daar et al, 1984;Mayer & Shlien, 1987;Bland & Whiting, 1989;Mayrhofer & Spargo, 1989 or can be induced to express Mhc class II under pathological conditions (Marley et al, 1987;Matthews et al, 1988;Masson & Perdue, 1990) or in response to interferon- (Steiniger et al, 1989). Furthermore, Du Pasquier & Flajnik (1990) noted that in Xenopus and especially in tadpoles, cell surface expression of Mhc class II in nonlymphoid cells was concentrated in locations in direct contact with the external environment.…”

Section: Discussionmentioning

confidence: 97%

Differing levels of Mhc class II β chain expression in a range of tissues from vaccinated and non-vaccinated Atlantic salmon (Salmo salarL.)

Koppang

Lundin

Press

et al. 1998

Fish & Shellfish Immunology

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“…Some limited expression by intestinal epithelial cells also occurs (6,31). However, during nematode infection the pattern of MHC II expression within the intestine is altered (28). More professional APC are recruited to the gut, and nonimmune cells such as smooth muscle cells (21,22) and glial cells (2) may be stimulated by inflammatory cytokines to express MHC II and possibly play an active role in antigen presentation (2,21,22).…”

mentioning

confidence: 99%

CD4 T Cells and Major Histocompatibility Complex Class II Expression Influence Worm Expulsion and Increased Intestinal Muscle Contraction duringTrichinella spiralisInfection

Vallance¹,

Galeazzi²,

Collins³

et al. 1999

Infect Immun

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Expulsion of intestinal nematode parasites and the associated increased contraction by intestinal muscle are T cell dependent, since both are attenuated in athymic rodents. The CD4 T-cell subset has been strongly associated with worm expulsion; however, the relationship between these cells, antigen presentation, and worm expulsion is not definitive and the role of these factors in intestinal muscle hypercontractility has not been defined. We infected C57BL/6, athymic, CD4-deficient, CD8α-deficient, and major histocompatibility complex class II (MHC II)-deficient (C2d) mice with Trichinella spiralis larvae. We examined intestinal worm numbers, longitudinal muscle contraction, and MHC II expression. Numerous MHC II-positive cells were identified within the muscularis externa of infected but not uninfected C57BL/6 mice. C57BL/6 and CD8α-deficient mice developed large increases in muscle contraction, expelling the parasite by day 21. Athymic and C2d mice exhibited much smaller increases in muscle contraction and delayed parasite expulsion. CD4-deficient mice exhibited intermediate levels of muscle contraction and delayed parasite expulsion. To further examine the role of MHC II and CD4 T cells, we irradiated C2d mice and reconstituted them with C57BL/6 bone marrow alone or with C57BL/6 CD4 T cells. C57BL/6 bone marrow alone did not affect muscle function or worm expulsion in recipient C2d mice. Partial CD4 T-cell reconstitution was sufficient to restore increased muscle contraction but not worm expulsion. Thus, hematopoietic MHC II expression alone is insufficient for the development of muscle hypercontractility and worm expulsion, but the addition of even small numbers of CD4 T cells was sufficient to induce intestinal muscle pathophysiology.

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Changes in distribution of Ia antigen on epithelium of the jejunum and ileum in rats infected with Nippostrongylus brasiliensis

Cited by 19 publications

References 22 publications

Mechanisms of Parasite Antigen Uptake from the Intestine of Rats Infected with the Nematode Nippostrongylus brasiliensis.

Mechanisms of Parasite Antigen Uptake from the Intestine of Rats Infected with the Nematode Nippostrongylus brasiliensis.

Differing levels of Mhc class II β chain expression in a range of tissues from vaccinated and non-vaccinated Atlantic salmon (Salmo salarL.)

CD4 T Cells and Major Histocompatibility Complex Class II Expression Influence Worm Expulsion and Increased Intestinal Muscle Contraction duringTrichinella spiralisInfection

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