Differing levels of Mhc class II β chain expression in a range of tissues from vaccinated and non-vaccinated Atlantic salmon (Salmo salarL.)

Koppang, Erling O.; Lundin, Maria; Press, Charles McL.; Rønningen, K.; Lie, Ø.

doi:10.1006/fsim.1997.0136

Cited by 64 publications

(42 citation statements)

References 35 publications

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“…Previous studies looking at di#erential expression of MHC class II genes in fish have shown varying levels of transcription in di#erent tissues and cells (Rodrigues et al, 1995;Koppang et al, 1998). Studies of the ontogeny of MHC class II expression in carp Cyprinus carpio, using RNA extracted from whole embryos, have shown that transcription increases with time (Rodrigues et al, in press).…”

Section: Discussionmentioning

confidence: 96%

Modulation of MHC class II expression in rainbow troutOncorhynchus mykissmacrophages by TNFα and LPS

Knight

Stet

Secombes

1998

Fish & Shellfish Immunology

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Section: Discussionmentioning

confidence: 96%

Modulation of MHC class II expression in rainbow troutOncorhynchus mykissmacrophages by TNFα and LPS

Knight

Stet

Secombes

1998

Fish & Shellfish Immunology

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“…We found a significant increase in C5aR expression in fish at 25°C, which supports the notion of an increased level of this arm of the immune system at higher temperatures. MHC class II receptors are expressed on the surface of antigen-presenting cells (Nath et al 2006), and increased expression of MHC II in the spleen and gills has been recorded after vaccination of salmon (Koppang et al 1998). However, we could not detect changes of MHC II expression in spleen and head kidney following immunization.…”

Section: Discussionmentioning

confidence: 99%

Temperature-dependent expression of immune-relevant genes in rainbow trout following Yersinia ruckeri vaccination

Raida¹,

Buchmann²

2007

Dis. Aquat. Org.

107

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The gene expression of immune-relevant genes in rainbow trout Oncorhynchus mykiss following vaccination with a bacterin of Yersinia ruckeri, a bacterial pathogen causing enteric red mouth disease (ERM), was investigated at 5, 15, and 25°C. Rainbow trout were immunized by i.p. injection of a water-based Y. ruckeri (serotype O1) bacterin, and gene expression profiles were compared to control groups injected with phosphate buffered saline (PBS). Blood and tissue samples (spleen and head kidney) were taken for subsequent analysis using solid phase enzyme-linked immunosorbent assay (ELISA) and real-time PCR, respectively. The up-regulation of cytokine genes was generally faster and higher at high water temperature, with major expression at 25°C. The proinflammatory cytokine interleukin (IL)-1β and interferon (IFN)-γ were significantly up-regulated in all immunized groups, whereas the cytokine IL-10 was only up-regulated in fish kept at 15 and 25°C. The gene encoding the C5a (anaphylatoxin) receptor was expressed at a significantly increased level in both head kidney and spleen of immunized fish. The secreted immunoglobulin M (IgM)-encoding gene was significantly up-regulated in the head kidney of immunized trout reared at 25°C, and a positive correlation (r = 0.663) was found between gene expression of secreted IgM in the head kidney and Y. ruckeri-specific antibodies in plasma measured by ELISA. However, no regulation of the teleost specific immunoglobulin T (IgT), which was generally expressed at a much lower level than IgM, could be detected. The study indicated that expression of both innate and specific adaptive immune-response genes are highly temperature-dependent in rainbow trout.

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“…40 However, previous investigations have shown that there is a retinal reaction to various cytokines and LPS in mammals, and that MHC class II expression may be induced in the Atlantic salmon after vaccination. 24 Because the retinal function is of vital importance to the animal, actions are taken to limit inflammatory responses as effectively as possible. 39 This is the probable explanation for the lack of MHC class II retinal induction in the present material.…”

Section: Discussionmentioning

confidence: 99%

“…Expression experiments showed that transcriptional regulatory mechanisms appeared similar to those in mammals. 22,24 The production of antisera recognizing the ␤ chain allowed us to investigate several tissues using immunohistochemic methods, and we suggested a similar distribution of MHC class II ϩ cells as that seen in mammals. 23 Fish possess camera-type eyes, similar to their mammalian counterparts.…”

mentioning

confidence: 99%

Granulomatous Uveitis Associated with Vaccination in the Atlantic Salmon

et al. 2004

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Abstract. This study addressed histologic and immunopathologic changes in ocular tissues and investigated the distribution of major histocompatibility class II (MHC class II)-positive cells in Atlantic salmon (Salmo salar) suffering from severe postvaccination disease. Twenty-nine fish with generalized inflammation, probably a result of vaccination, were investigated. One individual that had escaped vaccination was included in the study. Material was investigated by cultivation methods for fungi and bacteria. Histology using conventional staining procedures and immunohistochemistry with antisera against MHC class II ␤ chain were performed. No growth was observed from the cultivation investigations. Histology revealed occlusion of the lumen in the larger choroid vessels and in the choriocapillaris, inflammatory infiltrations and loss of structure in the choroid rete, and, in some cases, aggregations of multinucleated giant cells (MGC) and Splendore-Hoeppli material. Immunohistochemistry demonstrated massive MHC class II ϩ cellular infiltrations in the uveal tract. Such infiltrations were also seen in the ventral ciliary cleft, a condition that is associated with glaucoma. Immunoreactive cells included dendritelike cells, epithelioid cells, and MGCs. The endothelia of smaller vessels were frequently MHC class II ϩ , and immunoreactive infiltrations were seen in the optic nerve in several individuals. No pathologic changes were detected in the unvaccinated individual. In conclusion, generalized inflammatory reactions in fish may lead to severe ocular inflammation, occlusion of uveal vessels, and perivascular changes with MHC class II ϩ upregulation in cells in the uveal tract and optic nerve.

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Differing levels of Mhc class II β chain expression in a range of tissues from vaccinated and non-vaccinated Atlantic salmon (Salmo salarL.)

Cited by 64 publications

References 35 publications

Modulation of MHC class II expression in rainbow troutOncorhynchus mykissmacrophages by TNFα and LPS

Modulation of MHC class II expression in rainbow troutOncorhynchus mykissmacrophages by TNFα and LPS

Temperature-dependent expression of immune-relevant genes in rainbow trout following Yersinia ruckeri vaccination

Granulomatous Uveitis Associated with Vaccination in the Atlantic Salmon

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