Changes in deoxyribonucleoprotein during spermiogenesis in the bull

Etude cytophotométrique de l'ADN et des nucléoprotéines des spermatozoïdes du bélier : influence du séjour dans les voies génitales de la brebis

“…La structure condensée de la chromatine des spermatozoïdes de bélier résulte de la formation, pendant les dernières phases de la spermatogenèse, d'un complexe entre l'ADN et la protéine spécifique du spermatozoïde (BNSP) riche en cystéine et en arginine (Loir, 1972a ;Monfoort et al, 1973 (Esnault, 1965 ;Gledhill et al, 1966 ;Esnault et Nicolle, 1976 Orgebin-Crist, 1969).…”

The Proteins of Bovine Spermatozoa From the Caput and Cauda Epididymidis

“…Henricks & Mayer (1965a, b), Gledhill, Gledhill, Rigler & Ringertz (1966) and Ingles, Trevithick, Smith & Dixon (1966). A few of them are concerned with the changes associated with the alteration in the basic proteins of the nucleus from second spermatocyte or round spermatid to the spermatozoon (Alfert, 1956;Vendrely, Knobloch & Vendrely, 1957;Gledhill et al, 1966;Ingles et al, 1966).…”

“…A few of them are concerned with the changes associated with the alteration in the basic proteins of the nucleus from second spermatocyte or round spermatid to the spermatozoon (Alfert, 1956;Vendrely, Knobloch & Vendrely, 1957;Gledhill et al, 1966;Ingles et al, 1966). Two reports deal with the changes in the proteins during sperm passage through the epididymis.…”

“…Berry & Mayer (1960) reported an increase in the percentage of the basic amino acids and a decrease in that of the acidic acids of the basic proteins. Gledhill et al (1966) assumed that there is only a small increase in the percentage of basic amino acids in the basic proteins of the nucleus following the sperm passage through the genital tract.…”

Atypical Changes of Deoxyribonucleoprotein During Spermiogenesis Associated With a Case of Infertility in the Bull

“…Possibly, the infertility exhibited by the bull under consideration was the consequence of such a defective (immature) spermatozoal dnp complex. In a recent communication (Gledhill, Gledhill, Rigler & Ringertz, 1966) we reported that, normally during spermiogenesis in the bull, several pro¬ nounced cytochemical changes occur in the deoxyribonucleoprotein (dnp) complex. Summarily, these changes are: a decrease in number of phos¬ phate groups (DNA-PO4) available for binding by cationic dyes, an increase in total basicity of nuclear protein, an increase in protein-bound arginine and a reduction in stainability of dna by the Feulgen reaction.…”