Changes in content of ATP-related compounds, homarine, and trigonelline in marine invertebrates during ice storage.

Abstract

Marine invertebrates consisting of 9 species of mollusks, 7 of crustaceans and 3 of echinoderms, were analyzed for ATP-related compounds, homarine, and trigonelline during ice storage. The determination was carried out by a newly improved HPLC system which made it possible to separate and micro-quantify the 9 authentic ATP-related compounds. The results showed that besides adenine nucleotides, IMP was detected at the range of 0.1 to 5.5 ƒÊmol/g in the muscles of all species

Chemical Indices for Assessing Freshness of Shellfish during Storage

“…The high levels of A.E.C. and ATP in the muscle of the oyster, hard clam, and ark-shell obtained in this investigation indicated that specimens used in this study were in a better condition before the experiment than those in the report by Suwetja et al 10) In fact, in the preliminary experiment, we confirmed that the A.E.C. value at time 0 decreased when the speci mens were exposed to air for few days.…”

Changes in the contents of ATP and its related breakdown compounds in various tissues of oyster during frozen storage

“…The muscle of several species of bivalves was analyzed and it was reported that with no or less activity of AMP deaminase, IMP did not accumulate during storage at -5℃ and that ATP degradation proceeded as follows: ATP → ADP → AMP → AdR → HxR → Hx in surf clam Spisula sachalinensis and scallop Pecten yessoensis and P. albicans or ATP → ADP → AMP → AdR → Ad in ark shell Anodara broughtonii and abalone Haliotis discus (Saito, 1961;Arai, 1961b;Iwamoto et al, 1970). However, different reports are available on the accumulation of IMP in postmortem molluscan muscles (Suwetja et al, 1989;Sagedhal et al, 1997). It was detected that there was a large amount of IMP in edible parts of the short-neck clam Tapes japonica (Konosu et al, 1965).…”

“…It was detected that there was a large amount of IMP in edible parts of the short-neck clam Tapes japonica (Konosu et al, 1965). There are other reports that IMP, HxR and Hx were detected during storage in the muscle of the scallop Placopecten magellanicus (Hiltz and Dyer, 1970), oyster Crassostrea gigas (Suwetja et al, 1989;Sakaguchi et al, 1990), ark shell Anodara broughtonii, clam Meretrix lusoria and short-neck clam Tapes japonica (Sakaguchi et al, 1990). The results suggested the pathway: ATP → ADP → AMP → IMP → HxR.…”

“…As the rate and pattern of post-mortem changes in nucleotides and their related compounds differ considerably for fish species (Ryder, 1985), muscle types (Obatake et al, 1988), and factors related to handling and storage conditions (Surette et al, 1988), ATP degradation can be used as a good reference. Besides, the chemical assessment of the freshness in post-mortem storage has not yet been well established for bivalves (Suwetja et al, 1989;Yokoyama et al, 1992). Changes in levels of ATP-related compounds in adductor muscle, mantle, gill and the body trunk of the oyster were reported (Yokoyama et al, 1992).…”

Biochemical changes in oyster tissues and hemolymph during long-term air exposure

“…In marine shellfish, postmortem changes in the concentrations of ATP-related compounds (ARCs) have been investigated in detail to determine indices of freshness during storage [14][15][16][17][18][19][20][21]. Marine shellfish show seasonal changes in ARCs and free amino acids [22][23][24][25].…”