Changes in branchial Na+,K+-ATPase, metallo-thionein and P450 1A1 in dab Limanda limanda in the German Bight: indicators of sediment contamination?

Abstract

Na+,K+-ATPase activity, ouabain binding, metallothionein and P450 1Al were measured in gill tissue from the dab Lirnanda limanda caught from a contaminant gradient in the German Bight during the Bremerhaven Workshop. Metallothionein levels were highest at the most contaminated sites closest to the mouth of the Elbe and lowest at the outermost station on the Dogger Bank. Tissue levels of zinc and cadmium were also elevated at these sites but copper levels did not change along the gradient. P450 1Al levels, measured with an antibody probe, showed a complex pattern of induction which suggests an inhibitory effect at the most contaminated stations. Na+,Kc-ATPase was similar in fish from all of the stations but measurement of ouabain binding show that levels of the enzyme are elevated at the innermost and outer stations. Calculation of activity per binding site indicates that the turnover rates of the enzyme were lower at the most contaminated stations. The results show that the role of adaptive responses in the face of inhibition by contaminants is an important consideration in identifying biological effects. The data were analysed with respect to the contaminant burdens in the total and fine particulate fraction (< 63 pm) of the sediment and in the liver of dab caught at each station. The utility of biological effects measurement in the gill as an indicator of environmental contamination is discussed.

Detection of cytochrome P450 1A1 in North Sea dab liver and kidney

“…A strong correlation may therefore indicate only that other chemicals with similar physico-chemical properties are present and CO-vary with the measured substances. The different correlation profiles ob-served in different tissues (in gill tissue, a strong correlation was observed between P450 1A1 levels and PCB #52: Stagg et al 1992) indicate not only that various contaminants reach organs and tissues at different rates, but also that there may be organ-specific responses to the different chemicals. These observations are also interesting and deserve further study.…”

Ecotoxicology of Green Synthesized Silver Nanoparticles on Fresh Water Fish Mystus Gulio

“…ATPase plays a major role in transporting ions across cell thus maintaining osmotic balance in fish. ATPase was considered as a biomarker to assess the membrane fragility of the gills [46]. In heavy metal treated fishes inhibition or stimulation of ATP-ase activity was observed in gills [47].…”

Characterization of the microsomal epoxide hydrolase of hepatic microsomes of the common dab,Limanda limanda

“…The common dab, Limanda limanda, is frequently used (Goksayr et al 1991(Goksayr et al , 1992Lange et al 1992;Renton and Addison 1992;Eggens et al 1992;Stagg et al 1992Stagg et al , 1995 to assess the biological effectsof contamination in the North Sea and this species has been adopted into environmental monitoring progranames such as that of the North Sea Task Force (Stagg et al 1991). The choice of this species is determined by a number of factors including its wide-spread distribution, its benthic habit, the induction of xenobiotic-metabolising enzymes by common environmental pollutants and its apparent susceptibility to hepatic carcinogensis (Goksoyret al 1991(Goksoyret al , 1992Lange et al 1992;Renton and Addison 1992;Eggens et al 1992;Stagg et al 1992).…”

“…The choice of this species is determined by a number of factors including its wide-spread distribution, its benthic habit, the induction of xenobiotic-metabolising enzymes by common environmental pollutants and its apparent susceptibility to hepatic carcinogensis (Goksoyret al 1991(Goksoyret al , 1992Lange et al 1992;Renton and Addison 1992;Eggens et al 1992;Stagg et al 1992). The severity of liver lesions in fish populations seems to be related to the accumulation of environmental contaminants and polycyclic aromatic hydrocarbons (PAHs) may be particularly important (Kohler 1989;Malins et al 1989;Myers et al 199 I).…”