Changes in Automated Complete Blood Cell Count and Differential Leukocyte Count Results Induced by Storage of Blood at Room Temperature

Gulati, Gene; Hyland, L.; Kocher, William; Schwarting, Rolland

doi:10.5858/2002-126-0336-ciacbc

Cited by 65 publications

(21 citation statements)

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“…However, they did not observe the same increase in monocyte numbers. They concluded that these changes in the leukocyte sub-parameters could be due to cellular degeneration known as loss of individual cell properties and cell aging [9]. In our study, it supports Gulati et al 's findings.…”

Section: Discussionsupporting

confidence: 92%

Leukocyte stability in hemogram samples waiting at room temperature

Öztaş¹

2020

Int J Med Biochem

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Leukocyte stability in hemogram samples waiting at room temperature L aboratory testing process is divided into preanalytical, analytical and post-analytic stages. The vast majority of laboratory errors (up to 70%) emerge from the pre-analytical phase [1]. This phase is influenced by many variables, including the preparation of the patient before testing, the procedures used to collect and transport the biological specimens, as well as the time and storage conditions of blood samples before analysis. In particular, it was recently proven that the stability of many hematological parameters is strongly influenced by the storage temperature of the sample and the time elapsed between collection and analysis [2-4]. To give accurate and reliable results, the sample must be delivered to the laboratory at a certain temperature and time. In this study, we aimed to evaluate hourly changes of leukocyte parameters at room temperature for 24 hours. Materials and MethodsIn the study, 30 randomly selected hemogram samples (K3 -EDTA (tripotassium ethylenediamine-tetraacetate) anticoagulated blood specimens) from Kocaeli University Faculty of Medicine Central Laboratory were included. These samples were analyzed on the Beckman Coulter Unicel DXH 800 analyzer (Beckman Coulter, Miami, FL) immediately after being admitted to the laboratory (0. hour) and It was repeated after waiting in room temperature (23-25°C) for 2, 4, 8, 24 hours. In

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Section: Discussionsupporting

confidence: 92%

Leukocyte stability in hemogram samples waiting at room temperature

Öztaş¹

2020

Int J Med Biochem

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“…2 In human blood samples, the RBC count shows stability for up to 7 days. 19 However, RBC count stability may be species-related. Notably, the RBC count has been reported to change within 24 hours of sampling in dogs, 16 but remains consistent for 24 hours in rhesus macaques.…”

Section: Discussionmentioning

confidence: 99%

Stability of hematologic analytes in New Zealand white rabbits using an impedance‐based analyzer

Bibay

Margallo

2022

Veterinary Clinical Pathol

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Background The validity of hematologic data is crucial for correctly diagnosing certain conditions and providing accurate research results. However, delays in blood testing are inevitable. Objective This study aimed to determine whether prolonged storage at different temperatures affects CBC measurand stability in rabbit whole blood using the impedance‐based Scil Vet ABC hematology analyzer. Methods Hematologic tests were performed on whole blood samples collected from 20 New Zealand white rabbits at <1 hour post‐blood collection as the baseline and at 6, 24, 48, and 72 hours post‐blood collection, with aliquots stored at 21 and 4°C. CBC measurand stability was determined by comparing the obtained values at each time point to the baseline value. Statistically significant variations were compared with current quality specifications to determine if a meaningful bias existed. Results Hematocrit, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration remained stable for 72 hours in blood stored at 4°C. No meaningful bias was observed for mean corpuscular volume after 48 and 72 hours of storage at 4°C. Only the white blood cell count was stable for 72 hours at 21°C. The platelet count, hemoglobin concentration, and mean platelet volume were not stable at 6 hours post‐blood collection at either temperature. Conclusions The results of this study showed that the hematocrit can be tested for 72 hours in blood stored at 4°C, without a significant change from the baseline value. However, if all hematologic measurands are of interest, the immediate testing of blood samples, namely <1 hour post‐blood collection, remains highly recommended.

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“…Long-term storage of blood affects LDCs (Gulati et al, 2002). We therefore tested if the Epi-Blood-Count is also applicable after storage of blood samples for seven days at 4°C.…”

Section: Deconvolution Of Granulocytes Monocytes and Lymphocytes With Three Cpgsmentioning

confidence: 99%