Leukocyte stability in hemogram samples waiting at room temperature

Abstract

Leukocyte stability in hemogram samples waiting at room temperature L aboratory testing process is divided into preanalytical, analytical and post-analytic stages. The vast majority of laboratory errors (up to 70%) emerge from the pre-analytical phase [1]. This phase is influenced by many variables, including the preparation of the patient before testing, the procedures used to collect and transport the biological specimens, as well as the time and storage conditions of blood samples before analysis. In particular, it was recently proven that the stability of many hematological parameters is strongly influenced by the storage temperature of the sample and the time elapsed between collection and analysis [2-4]. To give accurate and reliable results, the sample must be delivered to the laboratory at a certain temperature and time. In this study, we aimed to evaluate hourly changes of leukocyte parameters at room temperature for 24 hours. Materials and MethodsIn the study, 30 randomly selected hemogram samples (K3 -EDTA (tripotassium ethylenediamine-tetraacetate) anticoagulated blood specimens) from Kocaeli University Faculty of Medicine Central Laboratory were included. These samples were analyzed on the Beckman Coulter Unicel DXH 800 analyzer (Beckman Coulter, Miami, FL) immediately after being admitted to the laboratory (0. hour) and It was repeated after waiting in room temperature (23-25°C) for 2, 4, 8, 24 hours. In