Changes in anti-Müllerian hormone concentrations in bitches throughout the oestrous cycle

Immunohistochemical and qPCR determination of the expression and serum level of anti‐Müllerian hormone in pre‐pubertal, intact and ovarian remnant syndrome detected bitches

“…Turna Yilmaz et al () thought that the serum AMH level in dogs was not affected by the cycle period and could be independent of gonadotropins. However, it was found that there was a high variation in AMH concentrations in dogs depending on both breed and individual by Walter et al (). It has also been reported that high levels of AMH concentration were determined between the three weeks before the onset of oestrus and six days before ovulation in dogs.…”

“…Similar to this study, Nagashima, Hansen, Songsasen, Travis, and Place () found that the AMH concentrations increased to coincide with the increase in the number of antral follicles in bitches. In addition, Walter et al () reported that AMH concentrations were increased in the late anoestrus, proestrus and oestrus and these could change due to the cycle in bitches. At the same time, AMH expression was found to be highest in the period of proestrus and oestrus.…”

“…Serum samples were stored at −20°C until analysis. The AMH test was performed using the Roche AMH kit (Detection limit: 0.010 ng/ml and Measuring range: 0.01–23 ng/ml) and the Roche Cobas e601 autoanalyzer using the ECLIA method (Hornakova et al, ; Walter et al, ).…”

Anti‐Müllerian hormone in dogs and cats reproduction

“…The range of the AMH concentrations varies among different test systems depending on the cross-reactivity of AMH with the antibodies used. In dogs, the canine-based ELISA tests Ansh Labs ® and EastBioPharm ® cMIS/AMH (Hollinshead et al, 2017;Pir Yagci, Pekcan, Polat, Kalender, & Macun, 2016;Themmen et al, 2016;Turna Yilmaz et al, 2015) provide higher AMH values than the human-based ELISA test Beckman Coulter ® AMH Gen II (Alm & Holst, 2018;Axnér & Holst, 2015;Gharagozlou, Youssefi, Akbarinejad, Mohammedkhani, & Shahpoorzadeh, 2014;Holst & Dreimanis, 2015;Pir Yagci et al, 2016;Place et al, 2011) and a human-based chemiluminescence immunoassay (Hornakova et al, 2017;Snoeck et al, 2017;Walter et al, 2018;Walter, Feulner, et al, 2019).…”

“…Significant changes in the AMH concentration throughout the oestrous cycle have been identified in bitches (Nagashima, Hansen, Songsasen, Travis, & Place, ; Walter, Feulner, et al, ). The main findings were a significant increase in the AMH concentration from late anoestrus up to 6 days before ovulation and a significant decrease starting 3 days before ovulation.…”