Long‐term reproductive evaluation of a bitch neonatally treated with a gonadotropin‐releasing hormone agonist

Abstract

In mammals, high concentrations of gonadotropins are necessary early in life to reach a full-sized pool of ovarian follicles at the age of puberty. This case report describes the reproductive status of a 28-month-old, 9.1 kg, crossbred bitch (Bitch 1) which had been treated neonatally with 18.8 deslorelin acetate subcutaneously for contraceptive purposes. The female had just passed pubertal oestrus when hormones were measured and then spayed. The ovaries were histologically assessed. Two crossbred bitches were used for comparisons. Bitch 1's anti-Müllerian hormone (AMH) and progesterone serum concentrations were 0.35 and 16.7 ng/ml, respectively. The total number of ovarian follicles and proportions were normal and the number of primary and antral follicles was increased. These findings might suggest an enhanced recruitment of the follicle pool to the growing population which could be related to the relatively low AMH concentrations.

Effect of a GnRH Antagonist on Anti-Müllerian Hormone and Follicular Population in Anestrous Bitches

“…Few studies have estimated the total number of preantral follicles in bitches. In line with previous reports, there was a large variation in the numbers of follicles among these females [20,21]. Furthermore, the mean number of follicles found here was similar to the ones previously reported using the same formula [20,21].…”

“…In line with previous reports, there was a large variation in the numbers of follicles among these females [20,21]. Furthermore, the mean number of follicles found here was similar to the ones previously reported using the same formula [20,21]. Minor differences among studies could be explained by the species variability and operators' bias.…”

“…The other two animals (Bitch 1 and Bitch 2) did not show a histological pattern of advanced follicular development. Grossly, the gonads of these females were in line with previous reports in similar size bitches [20].…”