Changes in Allele Frequencies at Storage Protein Loci of Winter Common Wheat under Climate Change

Козуб, Н. А.; Созинов, И. А.; Чайка, Володимир Миколайович; Sozinova, O. I.; Janse, L. A.; Blume, Ya. B.

doi:10.3103/s0095452720040076

Cited by 10 publications

(5 citation statements)

References 41 publications

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“…The predominant (p=0.52) allele of Gli-B1 locus revealed by PCR was Gli-B1.1 with the amplification fragment length of 369 bp, also frequent allele (p=0.32) was null allele (1RS.1BL translocation). Thereby the predominant allelic variants of gliadins in modern Ukrainian wheat varieties were Gli-B1b and Gli-B1l that corresponds to earlier studies [29,[31][32][33]. Gli-B1.2 allele occurs much less often than Gli-B1.1 and we revealed three types of different by length amplification fragments with primers specific to Gli-B1.2.…”

Section: Discussionsupporting

confidence: 86%

“…This allelic variant was predominant in sets of cultivars from The V. M. Remeslo Myronivka Institute of Wheat (MIW) (p=0.474) and Nosivska Selection and Breeding station (p=0.75). According to Kozub et al [29], Gli-B1l became the most frequent (p=0.439) in a set of Forest-Steppe wheat cultivars developed in 1996-2007 in MIW and is the most frequent (p=0.452) in wheat cultivars of MIW created after 2010 [31].…”

Section: Resultsmentioning

confidence: 98%

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Genetic variation of GLI-B1 locus in ukrainian bread wheat varieties and lines

2021

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To investigate polymorphism of Gli-B1 locus in modern Ukrainian bread wheat cultivars, to analyze the distribution of alleles and to compare the received data with the "core-collection of wheat cultivars" presented by Dr. E. Metakovsky. Methods. Eighty one bread wheat cultivars and lines from different plant breeding institutions and stations of Ukraine were tested using allele-specific primers to Gli-B1 locus developed by Zhang et al. [2003]. PCR products were fractionated in polyacrylamide gel (PAG) and then were stained by silver nitrate. Allelic variants of gliadins were analyzed by electrophoresis in acid polyacrylamide gel (APAGE). Results. Nine allelic variants of gliadins were revealed by APAGE and six alleles of Gli-B1 locus were detected by PCR-analysis. In 52 % of modern Ukrainian bread wheat cultivars we revealed Gli-B1b allelic variant, according to PCR -Gli-B1.1 allele with a 369 bp amplification fragment. In the genotypes of Ukrainian wheat cultivars, the 1RS.1BL translocation, which carries resistance genes, is frequent, as was detected by the absence of any amplification fragments with Gli-B1 primers. The correspondence between allelic variants of gliadins and alleles of Gli-B1 locus is discussed. Conclusions. DNA polymorphism of Gli-B1 locus examined in our research coincides with the diversity of allelic variants of gliadins, which were detected by APAGE method for Ukrainian bread wheat cultivar. However, PCR-analysis with applied primers carried out in this study does not distinguish the alleles that correspond to the Gli-B1c, Gli-B1g and Gli-B1e allelic variants of gliadins. The most common allele (52 %) for the investigated Ukrainian wheat varieties is Gli-B1.1 allele, which was characterized by the amplification fragment of 369 bp, and the presence of 1RS.1BL translocation, which corresponds to Gli-B1b and Gli-B1l allelic variants of gliadins obtained by APAGE, respectively.

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Section: Discussionsupporting

confidence: 86%

Section: Resultsmentioning

confidence: 98%

Genetic variation of GLI-B1 locus in ukrainian bread wheat varieties and lines

2021

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“…Так, поблизу Gli-A3 ідентифіковано мета-QTL, що відповідає за розвиток ознак кореневої системи, які, у свою чергу, пов`язані з адаптацією до посухи [15]. Підвищення частоти алеля Gli-A3a у групах сортів СГІ та ІР корелює з підвищенням середньорічної температури повітря в період створення сортів [12], що може вказувати на пов`язаність цього алеля з більшою адаптацією до умов посухи.…”

Section: результати та обговоренняunclassified

“…Аналізували 5-20 окремих зернівок кожного сорту. Також враховували дані про генотипи за Gli-A3, одержані в наших попередніх дослідженнях [11,12]. Алелі локусу Gli-A3 позначали згідно з [5], де омега-гліадин, кодований алелем Gli-A3с має найменшу рухливість (як у сорту Переяславка), а алель Gli-A3a -найвищу рухливість (як у сорту Миронівська 808), алель Gli-A3b -кодує білок з проміжним рівнем рухливості (сорт стандарт -Безоста 1), алель Gli-A3d -нуль-алель.…”

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Alleles at the Gli-A3 locus in groups of ukrainian winter common wheat cultivars of different origin

Kozub,

Sozinov,

Bidnyk

et al. 2023

Fakt. eksp. evol. org.

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Aim. The aim of the study was to analyze the diversity and frequencies of alleles at the minor gliadin locus Gli-A3 in groups of common winter wheat cultivars developed in different breeding institutions of Ukraine in different periods of time. Methods. Acid polyacrylamide gel electrophoresis of gliadins was carried out. To identify the Gli-A3 allele of the cultivar Myronivska storichna, segregation in a sample of F2 seeds from crossing with the cultivar Bezosta 1 was analyzed. Results. Alleles at Gli-A3 were identified in 511 Ukrainian cultivars. In addition to the alleles a-d, a new allele Gli-A3e encoding two omega-gliadins was identified in Myronivska storichna. The alleles a and b predominate in the total group of cultivars. Differences in the allele composition and frequencies were revealed between some groups of cultivars. A significant reduction in the frequency of Gli-A3b was detected for the groups of the Plant Breeding and Genetics Institute (PBGI) and the V. Ya. Yuryev Plant Production Institute (IPP) cultivars released after 2010. Conclusions. The frequencies of the predominant alleles a and b are similar in the groups of the Central Forest-Steppe cultivars in different periods of breeding. An increase in the frequency of the allele a was noted for the groups of PBGI (the Steppe) and IPP (the Eastern Forest-Steppe) cultivars, which may indicate its adaptive value under conditions of an increase in the average annual temperature in these zones.

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“…Одним із вдалих способів збагачення геноплазми пшениці чужинними генетичними компонентами міжсортовою гібридизацією є використання пшенично-житніх транслокацій (ПЖТ) [2]. Нині набувають поширення сорти пшениці озимої з ПЖТ, які характеризуються підвищеним адаптивним потенціалом та мають попит у виробництві, а для селекції це вихідний матеріал [3,4].…”

Section: агрономіяunclassified

Breeding and genetic features of the manifestation of the number of grains per main ear manifestation in hybrids with 1BL.1RS and 1AL.1RS wheatrye translocations in the Forest Steppe of Ukraine

et al. 2022

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The study examines the level of manifestation of the heterosis effect in hybrid populations, which increases the efciency of the selection process of winter wheat based on the number of grains in the main ear. 30 hybrid combinations were created using a complete diallel crossings scheme for six varieties of soft winter wheat with wheat-rye translocations (WRT): 1AL.1RS – Ekspromt, Zolotokolosa, Kolumbiia and 1BL.1RS – Kalynova, Svitanok Myronivskyi, Lehenda Myronivska. The Svitanok Myronivskyi variety used in crossbreeding gave the hybrids inheriting the studied trait mainly by additive effect, while with the use of Ekspromt, Zolotokolosa, Lehenda Myronivska, Kalynova, Kolumbiia varieties the hybrids inherited the trait by non-additive effect. The best in terms of the number of cases with high effects of total combining ability (TCA) on the number of grains per main ear were the WRT-carriers varieties: 1BL.1RS – Lehenda Myronivska, 1AL.1RS – Zolotokolosa and Kolumbiia. Regardless of the weather conditions of the year, a heterosis effect was found in the 1BL.1RS / 1BL.1RS crossing group and transgressive forms were selected in the following generations. The highest value of the trait was found in the following populations: F2 – Zolotokolosa / Kolumbiia, Svitanok Myronivskyi / Ekspromt (32.1 % each), Kalynova / Zolotokolosa (31.7 %); F3 – Kolumbiia / Zolotokolosa (41.5 %), Kolumbiia / Ekspromt (36.5 %) and others. In most of them, the translocation 1AL.1RS carrier varieties were the parent components. Key words: soft winter wheat, wheat-rye translocations, number of grains per main ear, general combining ability, specifc combining ability, heterosis, transgressions.

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Changes in Allele Frequencies at Storage Protein Loci of Winter Common Wheat under Climate Change

Cited by 10 publications

References 41 publications

Genetic variation of GLI-B1 locus in ukrainian bread wheat varieties and lines

Genetic variation of GLI-B1 locus in ukrainian bread wheat varieties and lines

Alleles at the Gli-A3 locus in groups of ukrainian winter common wheat cultivars of different origin

Breeding and genetic features of the manifestation of the number of grains per main ear manifestation in hybrids with 1BL.1RS and 1AL.1RS wheatrye translocations in the Forest Steppe of Ukraine

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