Changes at the 3′-untranslated region stabilize Rubisco activase transcript levels during heat stress in Arabidopsis

DeRidder, Benjamin P.; Shybut, Mikel; Dyle, Michael C.; Kremling, Karl A.; Shapiro, Mariya B.

doi:10.1007/s00425-012-1623-0

Cited by 25 publications

(23 citation statements)

References 76 publications

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“…DeRidder et al (2012) recently showed that AtRCAβ transcripts lacking their native 3′-UTR were significantly more stable than their full-length counterparts, indicating post-transcriptional regulation might participate in controlling of RCA expression in Arabidopsis . In addition, acclimation of RCA expression in Arabidopsis appeared to be due to changes in mRNA stability, not to increased rates of transcription.…”

Section: Discussionmentioning

confidence: 99%

Variation in Rubisco activase (RCAβ) gene promoters and expression in soybean [Glycine max (L.) Merr.]

Chao

Yin

Hao³

et al. 2013

Journal of Experimental Botany

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Section: Discussionmentioning

confidence: 99%

Variation in Rubisco activase (RCAβ) gene promoters and expression in soybean [Glycine max (L.) Merr.]

Chao

Yin

Hao³

et al. 2013

Journal of Experimental Botany

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“…For example, Rca expression is driven by a constitutive promoter in the Arabidopsis rwt43 transformant, whereas Rca expression in the wild type exhibits a strong circadian rhythm (Liu et al, 1996). This difference in the nature of the Rca promoter, as well as differences in the 39 untranslated region between the native Rca gene and the rwt43 transgene, are likely to affect the expression of Rca under the fluctuating light regime (DeRidder et al, 2012) and might influence the expression of other photosynthetic genes. Still, the data presented here suggest a new strategy for enhancing photosynthetic performance under variable light environments based on altering the regulatory properties of Rca to increase the rate of photosynthetic induction.…”

Section: The Functional Significance Of Regulating Rcamentioning

confidence: 99%

The Regulatory Properties of Rubisco Activase Differ among Species and Affect Photosynthetic Induction during Light Transitions

2013

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Rubisco's catalytic chaperone, Rubisco activase (Rca), uses the energy from ATP hydrolysis to restore catalytic competence to Rubisco. In Arabidopsis (Arabidopsis thaliana), inhibition of Rca activity by ADP is fine tuned by redox regulation of the a-isoform. To elucidate the mechanism for Rca regulation in species containing only the redox-insensitive b-isoform, the response of activity to ADP was characterized for different Rca forms. When assayed in leaf extracts, Rubisco activation was significantly inhibited by physiological ratios of ADP to ATP in species containing both a-Rca and b-Rca (Arabidopsis and camelina [Camelina sativa]) or just the b-Rca (tobacco [Nicotiana tabacum]). However, Rca activity was insensitive to ADP inhibition in an Arabidopsis transformant, rwt43, which expresses only Arabidopsis b-Rca, although not in a transformant of Arabidopsis that expresses a tobacco-like b-Rca. ATP hydrolysis by recombinant Arabidopsis b-Rca was much less sensitive to inhibition by ADP than recombinant tobacco b-Rca. Mutation of 17 amino acids in the tobacco b-Rca to the corresponding Arabidopsis residues reduced ADP sensitivity. In planta, Rubisco deactivated at low irradiance except in the Arabidopsis rwt43 transformant containing an ADP-insensitive Rca. Induction of CO 2 assimilation after transition from low to high irradiance was much more rapid in the rwt43 transformant compared with plants containing ADP-sensitive Rca forms. The faster rate of photosynthetic induction and a greater enhancement of growth under a fluctuating light regime by the rwt43 transformant compared with wild-type Arabidopsis suggests that manipulation of Rca regulation might provide a strategy for enhancing photosynthetic performance in certain variable light environments.

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“…In this study, we further observed that Zmrca2 was more highly expressed than Zmrca1 in six randomly selected maize inbred lines under nonstress conditions (data not shown). In cotton and Arabidopsis plants acclimated to heat stress, stabilization of the RCA transcript levels was linked to the production of transcripts with shorter 39 UTRs (DeRidder and Salvucci, 2007;DeRidder et al, 2012). The Zmrca1 transcript has a shorter 39 UTR than the Zmrca2 transcript.…”

Section: Posttranscriptional Regulation Of the Rca Genementioning

confidence: 99%

Characterization of Rubisco Activase Genes in Maize: An α-Isoform Gene Functions alongside a β-Isoform Gene

et al. 2014

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Rubisco activase (RCA) catalyzes the activation of Rubisco in vivo and plays a crucial role in regulating plant growth. In maize (Zea mays), only b-form RCA genes have been cloned and characterized. In this study, a genome-wide survey revealed the presence of an a-form RCA gene and a b-form RCA gene in the maize genome, herein referred to as ZmRCAa and ZmRCAb, respectively. An analysis of genomic DNA and complementary DNA sequences suggested that alternative splicing of the ZmRCAb precursor mRNA (premRNA) at its 39 untranslated region could produce two distinctive ZmRCAb transcripts. Analyses by electrophoresis and matrix-assisted laser desorption/ionization-tandem time-of-flight mass spectrometry showed that ZmRCAa and ZmRCAb encode larger and smaller polypeptides of approximately 46 and 43 kD, respectively. Transcriptional analyses demonstrated that the expression levels of both ZmRCAa and ZmRCAb were higher in leaves and during grain filling and that expression followed a specific cyclic day/night pattern. In 123 maize inbred lines with extensive genetic diversity, the transcript abundance and protein expression levels of these two RCA genes were positively correlated with grain yield. Additionally, both genes demonstrated a similar correlation with grain yield compared with three C 4 photosynthesis genes. Our data suggest that, in addition to the b-form RCA-encoding gene, the a-form RCA-encoding gene also contributes to the synthesis of RCA in maize and support the hypothesis that RCA genes may play an important role in determining maize productivity.

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Changes at the 3′-untranslated region stabilize Rubisco activase transcript levels during heat stress in Arabidopsis

Cited by 25 publications

References 76 publications

Variation in Rubisco activase (RCAβ) gene promoters and expression in soybean [Glycine max (L.) Merr.]

Variation in Rubisco activase (RCAβ) gene promoters and expression in soybean [Glycine max (L.) Merr.]

The Regulatory Properties of Rubisco Activase Differ among Species and Affect Photosynthetic Induction during Light Transitions

Characterization of Rubisco Activase Genes in Maize: An α-Isoform Gene Functions alongside a β-Isoform Gene

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