Variation in Rubisco activase (RCAβ) gene promoters and expression in soybean [Glycine max (L.) Merr.]

Chao, Maoni; Yin, Zhitong; Hao, Dejun; Zhang, Jinyu; Song, Haina; Ning, Ailing; Xu, Xiaoxia; Yu, Deyue

doi:10.1093/jxb/ert346

Cited by 39 publications

(64 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These data support the hypothesis that RCA genes play an important role in determining plant productivity. A similar result was observed in our recent studies in soybean, in which the transcript abundance of two RCA genes was positively correlated with grain yield (Yin et al, 2010;Chao et al, 2014).…”

Section: The Expression Levels Of Zmrcaa and Zmrcab Could Modulate Grsupporting

confidence: 91%

“…Both the maize in this study and the soybean in the previous studies (Yin et al, 2010;Chao et al, 2014) were grown under natural conditions and thus were exposed to a highly variable light environment each day. Plants with higher levels of RCA gene expression are thought to exhibit a more rapid increase in photosynthesis following an increase in light intensity, which could have resulted in increased grain yield.…”

Section: The Expression Levels Of Zmrcaa and Zmrcab Could Modulate Grmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of Rubisco Activase Genes in Maize: An α-Isoform Gene Functions alongside a β-Isoform Gene

et al. 2014

Self Cite

View full text Add to dashboard Cite

Rubisco activase (RCA) catalyzes the activation of Rubisco in vivo and plays a crucial role in regulating plant growth. In maize (Zea mays), only b-form RCA genes have been cloned and characterized. In this study, a genome-wide survey revealed the presence of an a-form RCA gene and a b-form RCA gene in the maize genome, herein referred to as ZmRCAa and ZmRCAb, respectively. An analysis of genomic DNA and complementary DNA sequences suggested that alternative splicing of the ZmRCAb precursor mRNA (premRNA) at its 39 untranslated region could produce two distinctive ZmRCAb transcripts. Analyses by electrophoresis and matrix-assisted laser desorption/ionization-tandem time-of-flight mass spectrometry showed that ZmRCAa and ZmRCAb encode larger and smaller polypeptides of approximately 46 and 43 kD, respectively. Transcriptional analyses demonstrated that the expression levels of both ZmRCAa and ZmRCAb were higher in leaves and during grain filling and that expression followed a specific cyclic day/night pattern. In 123 maize inbred lines with extensive genetic diversity, the transcript abundance and protein expression levels of these two RCA genes were positively correlated with grain yield. Additionally, both genes demonstrated a similar correlation with grain yield compared with three C 4 photosynthesis genes. Our data suggest that, in addition to the b-form RCA-encoding gene, the a-form RCA-encoding gene also contributes to the synthesis of RCA in maize and support the hypothesis that RCA genes may play an important role in determining maize productivity.

show abstract

Section: The Expression Levels Of Zmrcaa and Zmrcab Could Modulate Grsupporting

confidence: 91%

Section: The Expression Levels Of Zmrcaa and Zmrcab Could Modulate Grmentioning

confidence: 99%

Characterization of Rubisco Activase Genes in Maize: An α-Isoform Gene Functions alongside a β-Isoform Gene

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…In higher plants, the active state of Rubisco is strictly dependent on Rubisco activase (RCA). RCA is a chaperone-like protein of the AAA+ family, which utilizes ATP hydrolysis to mechanically modify the conformation of Rubisco and facilitate dissociation of tight-binding inhibitors of the active site (Chao et al, 2014). Most species studied contain two isoforms of RCA, α-(46-48 kDa) and β-isoform (41-43 kDa), from a single alternatively spliced pre-mRNA that is transcribed from a single RCA gene (Bayramov and Guliyev, 2014).…”

Section: Discussionmentioning

confidence: 99%

Gene expression changes in response to drought stress in Ilex paraguariensis leaves

Acevedo¹,

Ruíz²,

Sansberro³

2016

Plant Omics

View full text Add to dashboard Cite

Ilex paraguariensis St. Hil. is recognized as a shade-tolerant tree, cultivated in north-eastern Argentina, south-eastern Brazil, and Paraguay. Its leaves and young shoots are industrialised and; hence, being used to prepare a traditional tea-like infusion (mate-tea) with various medicinal benefits. Under the prevailing agro-climatic conditions in NE Argentina and with the occurrence of high temperatures and scarce rains during spring or summer, I. paraguariensis plants are subjected to water stress, reducing their yield. Differential Display was used to compare differences in gene expression between stressed and unstressed (control) potted plants. We found 52 differentially expressed transcripts (DETs), analysing banding patterns of Differential Display polyacrylamide gels, in which 38 DETs were induced, and 14 DETs were repressed in response to drought. Sequence comparisons revealed that 19% of the fragments showed no homologies, whereas 40.5 % of the total number of fragments had strong similarities with database entries of known functions. The other DETs belonged to unclassified proteins or genes with unknown functions. Interestingly, 12% of the DNA sequences were related to signal transduction, and 2% of the sequences were identified as transcription factors. Approximately, 12% of the annotated sequences were involved in stress tolerance and metal homeostasis, while 7% of the DETs were associated with photosynthesis. Other groups, representing 12%, included those involved in secondary metabolism, lignin biosynthesis, cell walls and RNA stabilization/chaperone activity. When integrated with physiological data, our results suggest that the transcriptional activity of some of the studied genes could be related to the degree of drought tolerance/susceptibility of I. paraguariensis.

show abstract

“…In addition, 15 SNPs developed from the GmRCAb gene promoter sequence were included in this study (Supplementary Table 2 in Online Resource 1) and were genotyped by re-sequencing the gene in this population. GmRCAb encodes a soybean Rubisco activase (RCA) b subunit (Chao et al 2014). Overall, 1433 SNPs were used for the subsequent analyses.…”

Section: Snp Genotypingmentioning

confidence: 99%

“…The gene for qSCCW18-3 was GmRCAb, encoding a soybean Rubisco activase (RCA) b subunit, whose expression levels were positively correlated with U PSII , qP, and seed yield in our previous reports (Chao et al 2014;Yin et al 2010). According to the polymorphism in the GmRCAb promoter sequence, the alleles of GmRCAb were classified into two groups: high-expression Group A type and low-expression Group B type (Chao et al 2014).…”

Section: Population Structure and Relative Kinshipmentioning

confidence: 99%

Mapping quantitative trait loci associated with soybean resistance to common cutworm and soybean compensatory growth after defoliation using SNP marker-based genome-wide association analysis

Wang

Yan

et al. 2015

Mol Breeding

Self Cite

View full text Add to dashboard Cite

Resistance and tolerance are different plant defense mechanisms against herbivores. In this study, four compensatory growth (tolerance) indexes-the ratios of treatment to control for plant height (RPH), branching number of the stem (RBN), seed number per plant (RSN), and seed weight per plant (RSW)-and three resistance indexes-larval weight (LW), pupal weight (PW), and larva-consumed leaf weight (LCL)-were used to evaluate soybean (Glycine max (L.) Merr) defense against common cutworm (CCW; Spodoptera litura Fabricius). The phenotypic variations of seven traits among 170 accessions were significant (ANOVA, P \ 0.01). Five of the seven traits had a large variation ([20 %) in this population. The decay distance of linkage disequilibrium was approximately 639 kb on average in intrachromosomal regions. Approximately 80 % of the intervals between two neighboring single-nucleotide polymorphisms were less than 600 kb. The natural population was divided into two subpopulations with little population structure. The genetic relationship among the accessions was distant. Fifty-one quantitative trait loci (QTLs) were significantly associated with the seven traits: six for RPH, nine for RBN, seven for RSN/RSW, one for RBN/RSN/RSW, six for LW, nineteen for LCL, and three for PW (-Log P C 2.00, P B 0.01). Single QTLs contributed 2.8-11 % of the phenotypic variance. Three QTLs were co-associated with soybean resistance and compensatory growth at the 0.05 significant level. Of these three QTLs, the qSCCW18-3 located in the promoter sequence of the soybean Rubisco activase b subunit gene (GmRCAb) and its resistance allele reduced LW and increased plant height.

show abstract

Variation in Rubisco activase (RCAβ) gene promoters and expression in soybean [Glycine max (L.) Merr.]

Abstract: Variation in Rubisco activase gene promoters (RCAβ) could affect diversity of expression level, which provided a new approach for enhancing soybean productivity by altering the expression level of RCAβ

Cited by 39 publications

References 68 publications

Characterization of Rubisco Activase Genes in Maize: An α-Isoform Gene Functions alongside a β-Isoform Gene

Characterization of Rubisco Activase Genes in Maize: An α-Isoform Gene Functions alongside a β-Isoform Gene

Gene expression changes in response to drought stress in Ilex paraguariensis leaves

Mapping quantitative trait loci associated with soybean resistance to common cutworm and soybean compensatory growth after defoliation using SNP marker-based genome-wide association analysis

Contact Info

Product

Resources

About