Changed composition of metabolites in Solanum tuberosum subjected to osmotic stress in vitro: Is sorbitol taken up?

Bündig, Christin; Blume, Christian; Peterhänsel, Christoph; Winkelmann, Traud

doi:10.1007/s11240-016-1042-1

Cited by 12 publications

(8 citation statements)

References 43 publications

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“…It was found that both genotypes displayed an uptake sorbitol. But it is unknow if it is also metabolized or only stored in potatoes (Bündig et al, 2016). In our work there were differences due the genotype factor in response to the treatments but the magnitude of the differences in growing are not as marked as the differences produced by the concentration of osmotic agent.…”

Section: Discussionmentioning

confidence: 46%

“…This suggests that sorbitol has some effects that allow or even promote some type of growing of cellular divisions, whereas mannitol is more restrictive to plant growth over Chilean native germplasm. Bündig et al (2016) demonstrate that two potato genotypes, divergently reacting to 0.2 M sorbitol in vitro act differently with the applied stress. The authors suggest that the tolerant genotype might have adapted to the applied stress in vitro after 11 d, whereas the sensitive genotype still suffers from osmotic stress and takes countermeasures in building up new metabolites and proteins for protection.…”

Section: Discussionmentioning

confidence: 84%

“…However, the percentage of mortality of explants was higher with 60 g L -1 , either in sorbitol or mannitol reaching 26.6% with such concentration. An ideal osmotic agent would be non-toxic, non-penetrating yet reducing the osmotic potential of the medium (Bündig et al, 2016). Increases in acyclic polyols such as mannitol and sorbitol have been observed in response to stress in many plants.…”

Section: Discussionmentioning

confidence: 99%

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Slow growth in vitro culture for conservation of Chilotanum potato germplasm

Muñoz

Díaz

Reinún

et al. 2019

Chil. j. agric. res.

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To keep potato (Solanum tuberosum L.) germplasm accessions disease-free and available for use, these are conserved as in vitro microplants under tissue culture conditions. The management of in vitro plants is labor intensive due to the necessity of periodic transferring of explants to new containers and fresh medium (sub culturing). The effectiveness of MS medium supplemented with sorbitol or mannitol in conservation of potato germplasm corresponding to different genotypes from Chilean native landraces (Chilotanum group) was investigated. Growth curves for modelling, describing and predicting shoot elongation of in vitro potato plants through the time in different culture media were developed as a tool to plan subculture labor for refreshing explants to new media. In MS medium without osmotic active compounds the rate of shoot elongation (k) was 1.12-1.45 cm wk-1 with 0% mortality. In media supplemented with 20, 40, and 60 g L-1 sorbitol, k value ranged between 0.58-0.35, 0.42-0.27 and 0.09-0.05 cm wk-1 , respectively. Mortality was 0%, 13%, and 26% for such treatments. In case of mannitol, k value ranged between 0.14-0.25, 0.065-0.11 and 0.042-0.068 cm wk-1 with 3%, 6%, and 26% mortality for 20, 40, and 60 g L-1 , respectively. These data can be used to predict shoot elongation rate in different media that provide several alternatives of speed of growth. The information allows to design an adequate strategy for organizing the work in a potato germplasm bank of S. tuberosum, Chilotanum group.

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Section: Discussionmentioning

confidence: 46%

Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Slow growth in vitro culture for conservation of Chilotanum potato germplasm

Muñoz

Díaz

Reinún

et al. 2019

Chil. j. agric. res.

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“…Metabolite extraction and sample preparation was performed as described by Bündig and colleagues [21]. The samples were loaded on a 30 m VF-5ms GC column (Agilent, Santa Clara, USA) according to Lisec and colleagues [22].…”

Section: Methodsmentioning

confidence: 99%

Low CO2 induces urea cycle intermediate accumulation in Arabidopsis thaliana

et al. 2019

Self Cite

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The non-proteinogenic amino acid ornithine links several stress response pathways. From a previous study we know that ornithine accumulates in response to low CO2. To investigate ornithine accumulation in plants, we shifted plants to either low CO2 or low light. Both conditions increased carbon limitation, but only low CO2 also increased the rate of photorespiration. Changes in metabolite profiles of light- and CO2-limited plants were quite similar. Several amino acids that are known markers of senescence accumulated strongly under both conditions. However, urea cycle intermediates respond differently between the two treatments. While the levels of both ornithine and citrulline were much higher in plants shifted to 100 ppm CO2 compared to those kept in 400 ppm CO2, their metabolite abundance did not significantly change in response to a light limitation. Furthermore, both ornithine and citrulline accumulation is independent from sugar starvation. Exogenous supplied sugar did not significantly change the accumulation of the two metabolites in low CO2-stressed plants, while the accumulation of other amino acids was reduced by about 50%. Gene expression measurements showed a reduction of the entire arginine biosynthetic pathway in response to low CO2. Genes in both proline biosynthesis and degradation were induced. Hence, proline did not accumulate in response to low CO2 like observed for many other stresses. We propose that excess of nitrogen re-fixed during photorespiration can be alternatively stored in ornithine and citrulline under low CO2 conditions. Furthermore, ornithine is converted to pyrroline-5-carboxylate by the action of δOAT.

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“…Although non-targeted strategies have been the most-extended approach to evaluate changes in the metabolic profiles of food samples during food-processing, targeted analysis may also be very useful; this kind of approaches has been employed to evaluate the metabolic changes that take place in two starch potato genotypes in response to osmotic stress [130] or during avocado development and maturation [131].…”

Section: Ms-based Metabolomics For Food Traceabilitymentioning

confidence: 99%

Application of mass spectrometry-based metabolomics approaches for food safety, quality and traceability

Castro-Puyana

Pérez-Míguez

Montero

et al. 2017

TrAC Trends in Analytical Chemistry

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The always more-demanding fields of food safety, quality and traceability are continuously fostering the development of robust, efficient, sensitive and cost-effective analytical methodologies. Mass spectrometry-based metabolomics is a key tool nowadays with great potential in many analytical fields and has been demonstrated to be capable of facing some important challenges related to these areas within the food science domain. The main aim of this review is to present a critical overview of the most recent applications of MS-based metabolomics approaches for food quality, safety and traceability assessment, covering the most relevant works published from 2014 to 2017. Information about the different steps needed to develop a MS-metabolomics approach, i.e. sample treatment, analytical platform, and data processing, is also provided and discussed.

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Changed composition of metabolites in Solanum tuberosum subjected to osmotic stress in vitro: Is sorbitol taken up?

Cited by 12 publications

References 43 publications

Slow growth in vitro culture for conservation of Chilotanum potato germplasm

Slow growth in vitro culture for conservation of Chilotanum potato germplasm

Low CO2 induces urea cycle intermediate accumulation in Arabidopsis thaliana

Application of mass spectrometry-based metabolomics approaches for food safety, quality and traceability

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