Change in the regulation of enzyme synthesis under catabolite repression in Bacillus subtilis pleiotropic mutant lacking transketolase.

Flagella and motility alterations in Pseudomonas aeruginosa strains from patients with cystic fibrosis: relationship to patient clinical condition

“…Deficiency in flagellation has been reported previously in a Bacillus subtilis mutant by Sasajima and Kumada (28,29) and Sasajima et al (30). A defect in the cell surface structure of the B. subtilis mutant caused altered cell morphology, bacteriophage sensitivity, and nonflagellation.…”

Microbial Synthesis of d-Ribose: Metabolic Deregulation and Fermentation Process

“…The synthesis of enzymes that participate in o-mannitol catabolism, such as D-mannitol PEP-dependent PTS and D-mannitol-1-phosphate dehydrogenase, was hypersensitive to repression by D-glucose, D-gluconic acid, D-xylose, and L-arabinose in the tkt mutant strain B. subtilis BG 2607 (Sasajima and Kumada, 1981a). In contrast, the synthesis of enzymes that catalyze the conversion of D-sorbitol (D-sorbitol permease, D-sorbitol dehydrogenase) and glycerol (glycerol permease, glycerol kinase, and glycerol dehydrogenase) was insensitive to D-glucose repression (Sasajima and Kumada, 1981a). The changed regulation in enzyme synthesis seemed to be related to a defect in the cell surface structure of the tkt mutant, thereby generating several pleiotropic properties.…”

Fewer essential genes in mycoplasmas than previous studies suggest

“…Interestingly, orthologs of these three genes are nonessential in M. genitalium . The tkt gene coding for transketolase is essential in B. subtilis for growth in minimal medium when using glucose as the sole carbon source (Kobayashi et al , 2003), but is nonessential when alternative carbon sources and aromatic amino acids are available (Sasajima & Yoneda, 1974; Sasajima & Kumada, 1981). The finding that tkt (MYPU_5110) is nonessential in mycoplasmas is not surprising because of the rich medium required for growth.…”