Microbial Synthesis of d-Ribose: Metabolic Deregulation and Fermentation Process

Wulf, Peter De; Vandamme, Erick

doi:10.1016/s0065-2164(08)70462-3

Cited by 7 publications

(5 citation statements)

References 148 publications

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“…In comparison with that of organisms that naturally produce 5-carbon sugars or those that are improved by classical mutagenesis (1,4,5,12,36) or by the previously reported processes for xylitol production from D-glucose (19,21,23), the amounts of 5-carbon sugars and sugar alcohols produced by the recombinant S. cerevisiae strains of this study were low. One very likely reason is the low capacity of the PPP in S. cerevisiae; less than 4% of D-glucose is channeled through this pathway during respirofermentative metabolism (6,17).…”

Section: Discussioncontrasting

confidence: 64%

“…DXylulose 5-phosphate and D-ribulose 5-phosphate also serve as precursors for D-arabitol when the D-arabitol phosphate dehydrogenase from Enterococcus avium is applied (22,24). DRibose, a drug precursor, and erythritol, a sweetener, are commercially produced from D-glucose by the Bacillus, Aureobasidium, and Torula species with a yield of almost 50% (wt/ wt) of consumed D-glucose (5,12,14,16).…”

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confidence: 99%

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Metabolic Engineering of Saccharomyces cerevisiae for Conversion of d -Glucose to Xylitol and Other Five-Carbon Sugars and Sugar Alcohols

Toivari

Ruohonen

Miasnikov³

et al. 2007

Appl Environ Microbiol

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Recombinant Saccharomyces cerevisiae strains that produce the sugar alcohols xylitol and ribitol and the pentose sugar D-ribose from D-glucose in a single fermentation step are described. A transketolase-deficient S. cerevisiae strain accumulated D-xylulose 5-phosphate intracellularly and released ribitol and pentose sugars (D-ribose, Dribulose, and D-xylulose) into the growth medium. Expression of the xylitol dehydrogenase-encoding gene XYL2 of Pichia stipitis in the transketolase-deficient strain resulted in an 8.5-fold enhancement of the total amount of the excreted sugar alcohols ribitol and xylitol. The additional introduction of the 2-deoxy-glucose 6-phosphate phosphatase-encoding gene DOG1 into the transketolase-deficient strain expressing the XYL2 gene resulted in a further 1.6-fold increase in ribitol production. Finally, deletion of the endogenous xylulokinase-encoding gene XKS1 was necessary to increase the amount of xylitol to 50% of the 5-carbon sugar alcohols excreted.

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Section: Discussioncontrasting

confidence: 64%

mentioning

confidence: 99%

Metabolic Engineering of Saccharomyces cerevisiae for Conversion of d -Glucose to Xylitol and Other Five-Carbon Sugars and Sugar Alcohols

Toivari

Ruohonen

Miasnikov³

et al. 2007

Appl Environ Microbiol

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“…The condensation of formaldehyde with D -ribulose 5-phosphate (hexulose-6-phosphate synthase) generates 3-oxo-6-phosphate hexulose, which is next isomerized into D -fructose 6-phosphate (hexulose-6-phosphate isom- erase). Due to the high glycolytic activity in Bacillus spp., the latter is predominantly converted into glycolytic metabolites (1). A decreased concentration of D -ribose and an enhanced amount of glycolytic end-products (lactic acid, acetic acid, acetoin and 2,3-butanediol) in the presence of formic acid thus should illustrate the existence of a RuMP pathway in B. subtilis.…”

Section: Presence Of the Ribulose Monophosphate Pathway In Bacillus Smentioning

confidence: 99%

“…B. subtilis ATCC 21951 instead converts D -xylulose 5-phosphate (via D -ribulose 5-phosphate) into D -ribose 5-phosphate, which accumulates at the TKT-conversion point. To overcome feed-back inhibition effects exerted by this intermediate, the strain dephosphorylates D -ribose 5-phosphate and secretes it as D -ribose into the medium (1) ().…”

Section: Presence Of the Ribulose Monophosphate Pathway In Bacillus Smentioning

confidence: 99%

Presence of the ribulose monophosphate pathway in Bacillus subtilis

Wulf

1998

Microbiology

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“…D-ribose was shown to be synthesized by enzymatic hydrolysis from yeast RNA or by chemical synthesis from arabinose, glucose, xylose and gluconic acid in the early part of the twentieth century [11]. However, these methods were associated with numerous problems such as low product recovery efficiency, insufficient amount and high costs, which led to development of the fermentation method for large-scale D-ribose production.…”

Section: Introductionmentioning

confidence: 99%

Optimization of medium components for D-ribose production by transketolase-deficient Bacillus subtilis NJT-1507

Fang

Chen

et al. 2010

Korean J. Chem. Eng.

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Statistical experimental designs were used to optimize the composition of culture media for the production of D-ribose by Bacillus subtilis. A fractional factorial design 2 (5-2) was used to determine medium components that significantly affected D-ribose production. The concentrations of glucose and (NH 4 ) 2 SO 4 were the significant factors. Central composite design and response surface methodology were then used to estimate the quadratic response surface and determine the factor levels for maximum production of D-ribose. Finally, the optimal medium composition was obtained (g/L): glucose, 172.75; (NH 4 ) 2 SO 4 , 13.2; yeast powder, 4; corn steep liquor, 8 and MnSO 4 , 0.5. This optimization strategy increased D-ribose production from 73.21 g/L to 88.57 g/L, an increase of 22% compared with the original conditions. The D-ribose production yield to glucose concentration was also enhanced from 0.37 g/g to 0.52 g/g. Confirmatory experiments were also performed to demonstrate the accuracy of the model. Under the optimal medium using ammonia to control pH in a 5 L fermenter, the D-ribose yield was increased to 95.28 g/L after 3 days of cultivation at 37 o C.

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Microbial Synthesis of d-Ribose: Metabolic Deregulation and Fermentation Process

Cited by 7 publications

References 148 publications

Metabolic Engineering of Saccharomyces cerevisiae for Conversion of d -Glucose to Xylitol and Other Five-Carbon Sugars and Sugar Alcohols

Metabolic Engineering of Saccharomyces cerevisiae for Conversion of d -Glucose to Xylitol and Other Five-Carbon Sugars and Sugar Alcohols

Presence of the ribulose monophosphate pathway in Bacillus subtilis

Optimization of medium components for D-ribose production by transketolase-deficient Bacillus subtilis NJT-1507

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