Change in Some Glycoalkaloids of Potato under Different Storage Regimes

Kasnak, Cemal; Artık, Nevzat

doi:10.1007/s11540-018-9367-2

Cited by 7 publications

(7 citation statements)

References 26 publications

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“…Those potatoes processed at a later timepoint had the highest levels of GA, which suggest that the conditions at which the potatoes are stored induce the level of GA in the potatoes. This confirms a previous result showing that prolonged storage under either indirect sunlight, dark room, dark room with cooling, or under fluorescent light were all associated with increased levels of GA in potatoes [8]. The concentration of solanidine increased as well during the storage of the potatoes.…”

Section: Glycoalkaloids In Potato Protein Isolatessupporting

confidence: 91%

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Liquid Chromatography Mass Spectrometry Quantification of α-solanine, α-chaconine, and Solanidine in Potato Protein Isolates

Nielsen

Schmidt

Kristiansen

et al. 2020

Foods

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For potato proteins to be used as a food ingredient, the level of natural potato defense substances, the glycoalkaloids (GAs), should be limited. In this work, a method is developed for quantification of the two major potato GAs, α-solanine and α-chaconine, as well as for their aglycon form, solanidine, using liquid chromatography–mass spectrometry single quadrupole in single ion monitoring mode. Standard solutions of GA and a food-grade potato protein powder was used to validate the method. A linear correlation between GA concentration and the ion intensity of >0.995 was obtained for all standard solutions. Recovery of GA in spiked samples was within the range 82%–106%. The method for GA quantification was applied to a variety of potato protein isolates. The results showed that total GA increased during the storage of the potatoes. Washing the potato protein isolates using water at a sufficient level was shown to be able to reduce the amount of GA below the threshold of 150 µg g−1, as needed for human consumption.

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Section: Glycoalkaloids In Potato Protein Isolatessupporting

confidence: 91%

“…The concentrations of GAs is highest in the blossoms (3000-5000 mg/kg) compared to the sprouts (2000-4000 mg/kg) and peel (300-600 mg/kg), whereas the average level across the entire potato tubers is approximately 100 mg/kg [7]. Furthermore, the level of GAs increases during the storage of potatoes after harvest [8].…”

Section: Introductionmentioning

confidence: 99%

Liquid Chromatography Mass Spectrometry Quantification of α-solanine, α-chaconine, and Solanidine in Potato Protein Isolates

Nielsen

Schmidt

Kristiansen

et al. 2020

Foods

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“…), normal metabolic pathways change in potato, which lead to the synthesis of abnormal amounts of numerous novel compounds and secondary metabolites, including toxic glycoalkaloids (GA), at levels that exceed the amount found in healthy tissues, also accompanied by the synthesis of other not usually found in the absence of stress [38]. An important role in the natural disease resistance of tubers is played by GA (in particular, steroid glycosides α-Solanine, and α-Chaconine), being concentrated mainly in their integumentary tissues [37]. However, GA is also potentially health-threatening toxic compounds in potatoes that pose a serious potential hazard to humans [38,39].…”

Section: Discussionmentioning

confidence: 99%

“…Under unfavorable storage conditions (i.e. pathogen attack), normal metabolic pathways change in potato, which leads to the synthesis of abnormal amounts of numerous novel compounds and secondary metabolites, including glycoalkaloids (GA) (α-Solanine and α-Chaconine are 95% of the total GA), which is believed are associated with antimicrobial, antifungal activities [35,36], and play an important role in the disease resistance of tubers [37]. With that, elevated GA levels in potatoes (200 mg/kg of tubers are considered as critical) are toxic compounds that pose a serious potential hazard to humans [38,39].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Endophytic Bacteria Bacillus subtilis and Salicylic Acid on Some Resistance and Quality Traits of Stored Solanum tuberosum L. Tubers Infected with Fusarium Dry Rot

Lastochkina

Пусенкова

Garshina

et al. 2020

Plants

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The effect of endophytic Bacillus subtilis (strains 10-4, 26D) and their compositions with salicylic acid (SA) on some resistance and quality traits of stored potatoes infected with Fusarium dry rot were studied. The experiments were carried out on hydroponically grown Solanum tuberosum L. tubers that were infected before storage with Fusarium oxysporum and coated with B. subtilis 10-4, 26D with and without exogenous SA, and then stored for six months. It has been shown that 10-4, 26D, 10-4 + SA, and 26D + SA reduced in different levels (up to 30–50%) the incidence of F. oxysporum-caused dry rot (with the highest effect for 10-4 + SA). SA notably enhanced the positive effect of 10-4, while for 26D, such an effect was not observed. All of the tested treatments increased amylase (AMY) and AMY inhibitors activity in infected tubers, while decreased Fusarium-induced protease activity (except in the case of 10-4 + SA, which promoted a slight increase) was revealed. 10-4, 26D, and their compositions with SA decreased (in different degrees) the pathogen-caused lipid peroxidation, proline, and reducing sugars accumulation in potatoes after long-term storage. It was also discovered 10-4 and 26D, regardless of SA presence, decrease pathogen-induced glycoalkaloids α-Solanine and α-Chaconine accumulation and preserved increased levels of starch and total dry matter in infected stored potatoes. The findings indicate endophytic B. subtilis and its compositions with SA is a promising eco-friendly and bio-safe approach to cope with postharvest decays of potato during long-term storage; however, when developing preparations-compositions it should take into account the strain-dependent manner of B. subtilis action together with SA.

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“…antioxidant activities (Uchida et al 2017;Mahieddine et al 2018;Fratianni et al 2020) and steroidal alkaloids widely reported for its toxic effects. The surge in concentration levels of steroidal alkaloids (glycoalkaloids) is triggered by the exposure of Solanum plants to the sun's UV light or because of mechanical injury including peeling and slicing (Dao and Friedman 1994;Kasnak and Artik 2018. Some of the toxic effects of glycoalkaloids are due to anticholinesterase effects on the central nervous system (Caprioli et al 2014;Lelario et al 2019) and disruption of cell membranes (Blankemeyer et al 1998;Nepal and Stine 2019.…”

Section: Highlightsmentioning

confidence: 99%

Effect of Microwave-Assisted Aqueous Two-Phase Extraction of α-Solanine from S. retroflexum and Analysis on UHPLC-qTOF-MS

et al. 2022

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A new, fast and efficient method, hyphenated microwave-assisted aqueous two-phase extraction (MA-ATPE) was applied in the extraction of α-solanine from Solanum retroflexum. This environmentally friendly extraction method applied water and ethanol as extraction solvents. Central composite design (CCD) was performed which included numerical parameters such as time, mass of plant powder and microwave power. The categorical factors included the chaotrope — NaCl or the kosmotrope — Na2CO3. Fitting the central composite design response surface model to the data generated a quadratic model with a good fit (R2 = 0.920). The statistically significant (p < 0.05) parameters such as time and mass of plant powder were influential in the extraction of α-solanine. Quantification of α-solanine was achieved using a robust and sensitive feature of the ultra-high performance quadrupole time of flight mass spectrometer (UHPLC-qTOF-MS), multiple reaction monitoring (MRM). The optimized condition for the extraction of α-solanine in the presence of NaCl and Na2CO3 was a period of 1 min at a mass of 1.2 g using a microwave power of 40%. Maximal extraction of α-solanine was 93.50 mg kg−1 and 72.16 mg kg−1 for Na2CO3 and NaCl, respectively. The synergistic effect of salting-out and microwave extraction was influential in extraction of α-solanine. Furthermore, the higher negative charge density of the kosmotrope (Na2CO3) was responsible for its greater extraction of α-solanine than chaotrope (NaCl). The shorter optimal extraction times of MA-ATPE make it a potential technique that could meet market demand as it is a quick, green and efficient method for removal of toxic metabolites in nutraceuticals.

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Change in Some Glycoalkaloids of Potato under Different Storage Regimes

Cited by 7 publications

References 26 publications

Liquid Chromatography Mass Spectrometry Quantification of α-solanine, α-chaconine, and Solanidine in Potato Protein Isolates

Liquid Chromatography Mass Spectrometry Quantification of α-solanine, α-chaconine, and Solanidine in Potato Protein Isolates

The Effect of Endophytic Bacteria Bacillus subtilis and Salicylic Acid on Some Resistance and Quality Traits of Stored Solanum tuberosum L. Tubers Infected with Fusarium Dry Rot

Effect of Microwave-Assisted Aqueous Two-Phase Extraction of α-Solanine from S. retroflexum and Analysis on UHPLC-qTOF-MS

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