Change in antimicrobial susceptibility of Mycoplasma gallisepticum field isolates

Gharaibeh, Saad; Alrashdan, Mohammad S.

doi:10.1016/j.vetmic.2011.02.005

Cited by 24 publications

(23 citation statements)

References 25 publications

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“…Phenicols showed good in vitro activity against Mycoplasma species of poultry, but information about their effectiveness in waterfowl is lacking [26, 27]. In the present study, most of the M .…”

Section: Discussionmentioning

confidence: 61%

Antibiotic susceptibility profiles of Mycoplasma sp. 1220 strains isolated from geese in Hungary

et al. 2016

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BackgroundMycoplasma sp. 1220 can induce inflammation primarily in the genital and respiratory tracts of waterfowl, leading to serious economic losses. Adequate housing and appropriate antibiotic treatment are promoted in the control of the disease. The aim of the present study was to determine the in vitro susceptibility to thirteen different antibiotics and an antibiotic combination of thirty-eight M. sp. 1220 strains isolated from geese and a duck in several parts of Hungary, Central Europe between 2011 and 2015.ResultsHigh MIC50 values were observed in the cases of tilmicosin (>64 μg/ml), oxytetracycline (64 μg/ml), norfloxacin (>10 μg/ml) and difloxacin (10 μg/ml). The examined strains yielded the same MIC50 values with spectinomycin, tylosin and florfenicol (8 μg/ml), while enrofloxacin (MIC50 5 μg/ml), doxycycline (MIC50 5 μg/ml), lincomycin (MIC50 4 μg/ml) and lincomycin-spectinomycin (1:2) combination (MIC50 4 μg/ml) inhibited the growth of the bacteria with lower concentrations. Tylvalosin (MIC50 0.5 μg/ml) and two pleuromutilins (tiamulin MIC50 0.625 μg/ml; valnemulin MIC50 ≤ 0.039 μg/ml) were found to be the most effective drugs against M. sp. 1220. However, strains with elevated MIC values were detected for all applied antibiotics.ConclusionsValnemulin, tiamulin and tylvalosin were found to be the most effective antibiotics in the study. Increasing resistance was observed in the cases of several antibiotics. The results highlight the importance of testing Mycoplasma species for antibiotic susceptibility before therapy.

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Section: Discussionmentioning

confidence: 61%

Antibiotic susceptibility profiles of Mycoplasma sp. 1220 strains isolated from geese in Hungary

et al. 2016

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“…The exceptional sensitivity of MG isolates to tiamulin makes it an attractive selection as an effective antimicrobial agent in the field for poultry farms which do not incorporate ionophore antibiotics in their feed. In addition, similar findings showed it to be effective against MG isolates in chicken in Iran (Ghaleh Golab Behbahan et al, 2008) and Jordan (Gharaibeh and Al-Rashdan, 2011). In contrast, gentamycin demonstrated a high resistant level (91.67%), against all groups of MG isolates at low concentrations (MIC 90 = 16 µg/ml).…”

Section: Discussionmentioning

confidence: 54%

“…These isolates were divided into three groups, according to the specimen sites of birds suffering from respiratory distress: group A, group B and group C. According to the present results, tiamulin was shown the highest sensitivity percentage (93.33%) against various isolates of MG (Hannan, 2000;Islam et al, 2009;Gharaibeh and Al-Rashdan, 2011). The MIC 90 of tiamulin for group A, B and C of the MG isolates was 1 µg/ml (Table 5), which is possibly a result of the fact that it has not been formerly used to treat chicken in Saudi Arabia.…”

Section: Discussionmentioning

confidence: 71%

Serological, rapid molecular characterization and antibiogram resistance for field isolates of Mycoplasma Gallisepticum in chicken in Saudi Arabia

Elbehiry¹,

Aldubaib²,

Marzouk³

2017

AJVS

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Key words:Mycoplasma Gallisepticum, TaqMan RT-PCR assay, Antimicrobial resistance Mycoplasma Gallisepticum (MG) is considered as one of the most economically important mycoplasmal pathogens among poultry industry worldwide. This pathogen has various strains, therefore their detection using culture method is not sufficient. From this point of view, this study was designed to develop a novel TaqMan ® real-time polymerase chain reaction (TaqMan RT-PCR) assay for direct detection of MG using cytadhesin to encode a surface protein (mgc2) containing a TaqMan FAM-labeled minor groove binder probe that targets this gene and to compare it with conventional polymerase chain reaction and immunological methods. Using serial broth dilution methods against identified MG isolates, minimum inhibitory concentrations of various antimicrobial agents were detected. Throughout Al-Qassim region, Kingdom of Saudi Arabia, 208 specimens were collected from 18 commercial chicken broiler farms where respiratory diseases were present. Furthermore, 180 blood serum samples were investigated for serological diagnosis of MG. Serum plate agglutination and enzyme-linked immunosorbent assay techniques detected positive serological identification in 83 (46.11%) and 97 (53.88%) isolates, respectively. The sensitivity recorded for TaqMan RT-PCR was 10 -3 CFU/ml for MG template DNA. Results of TaqMan RT-PCR revealed 169 positive samples (81.25%), while 108 samples (51.92%) were identified as positive through conventional polymerase chain reaction assay. The results of MIC for 11 antimicrobial agents against 60 identified MG isolates indicated that all groups of MG exhibited a higher degree of sensitivity to tiamulin (93.33%) at low level of MIC 50 and MIC 90 (≤0.032 µg/ml) followed by tylosin (85%) and doxycycline (81.66%) with MIC 50 and MIC 90 ranged from ≤0.032 to 2 µg/ml; In contrast, gentamycin, tilmicosin, erythromycin, ciprofloxacin, oxytetracylcine and enrofloxacin did not show a higher activity at low concentrations. In conclusion, the developed TaqMan RT-PCR exhibited higher sensitivity and applicable accuracy than other molecular and serological techniques and thus could be highly recommended for the management of MG susceptibility and to facilitate implementation of effective treatment and prophylactic measures.

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“…However, in the past decade, in vitro susceptibility studies on mycoplasma have shown an increased resistance to the erythromycin [18,22,23]. Several studies have shown that a mixture of a 13 and 15membered ring macrolide (tulathromycin) and 16membered macrolides (tylosin) are known to be more effective against mycoplasma than the 14-membered macrolides [23,24]. We found that isolates of M. dispar isolated from clinically healthy and cattle with respiratory system disorders were sensitive to tulathromycin and tylosin.…”

Section: Resultsmentioning

confidence: 85%

“…It has been suggested that the 14 membered macrolides (erythromycin) were very effective against human mycoplasmal pneumonia caused by M. pneumonia [21]. However, in the past decade, in vitro susceptibility studies on mycoplasma have shown an increased resistance to the erythromycin [18,22,23]. Several studies have shown that a mixture of a 13 and 15membered ring macrolide (tulathromycin) and 16membered macrolides (tylosin) are known to be more effective against mycoplasma than the 14-membered macrolides [23,24].…”

Section: Resultsmentioning

confidence: 99%

Mycoplasma dispar prevalence in the upper respiratory tract of cattle and the antimicrobial susceptibility of the isolates

2015

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The aim of this study was to make a survey of the presence of Mycoplasma dispar on a cattle breeding farm and to determine antimicrobial susceptibility of the isolates.The study was carried out at a farm in Lithuania. Nasal swabs for bacteriological investigation were collected from ninety dairy, beef and mixed type of cattle from 90 to 300 days of age. Mycoplasma cultivation procedures were carried out using Friis selective media. To confirm the presence of Mollicutes class the polymerase chain reaction (PCR) was used. Isolates were identified according to biochemical and antigenic characteristics.The minimum inhibitory concentration of twenty field isolates of Mycoplasma dispar to tulathromycin, tylosin, lincomycin, enrofloxacin, florfenicol, and oxytetracycline was determined by using a micro-broth dilution method.Mycoplasma dispar was detected in the nasal cavity of 15 out of 84 clinically healthy animals (17.9 %), and in 5 out of 6 animals with respiratory disorders (83.3 %). The isolates were most susceptible to tulathromycin, lincomycin, enrofloxacin and florfenicol. Three (15 %) isolates were resistant to oxytetracycline.The susceptibility to oxytetracycline significantly differed between Mycoplasma dispar isolates compared to the susceptibility of tulathromycin (P < 0.001), lincomycin (P < 0.001) tylosin (P < 0.001), enrofloxacin (P < 0.001), and florfenicol (P < 0.001).

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Change in antimicrobial susceptibility of Mycoplasma gallisepticum field isolates

Cited by 24 publications

References 25 publications

Antibiotic susceptibility profiles of Mycoplasma sp. 1220 strains isolated from geese in Hungary

Antibiotic susceptibility profiles of Mycoplasma sp. 1220 strains isolated from geese in Hungary

Serological, rapid molecular characterization and antibiogram resistance for field isolates of Mycoplasma Gallisepticum in chicken in Saudi Arabia

Mycoplasma dispar prevalence in the upper respiratory tract of cattle and the antimicrobial susceptibility of the isolates

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