Antibiotic susceptibility profiles of Mycoplasma sp. 1220 strains isolated from geese in Hungary

Abstract

BackgroundMycoplasma sp. 1220 can induce inflammation primarily in the genital and respiratory tracts of waterfowl, leading to serious economic losses. Adequate housing and appropriate antibiotic treatment are promoted in the control of the disease. The aim of the present study was to determine the in vitro susceptibility to thirteen different antibiotics and an antibiotic combination of thirty-eight M. sp. 1220 strains isolated from geese and a duck in several parts of Hungary, Central Europe between 2011 and 2015.ResultsHigh MIC50 values were observed in the cases of tilmicosin (>64 μg/ml), oxytetracycline (64 μg/ml), norfloxacin (>10 μg/ml) and difloxacin (10 μg/ml). The examined strains yielded the same MIC50 values with spectinomycin, tylosin and florfenicol (8 μg/ml), while enrofloxacin (MIC50 5 μg/ml), doxycycline (MIC50 5 μg/ml), lincomycin (MIC50 4 μg/ml) and lincomycin-spectinomycin (1:2) combination (MIC50 4 μg/ml) inhibited the growth of the bacteria with lower concentrations. Tylvalosin (MIC50 0.5 μg/ml) and two pleuromutilins (tiamulin MIC50 0.625 μg/ml; valnemulin MIC50 ≤ 0.039 μg/ml) were found to be the most effective drugs against M. sp. 1220. However, strains with elevated MIC values were detected for all applied antibiotics.ConclusionsValnemulin, tiamulin and tylvalosin were found to be the most effective antibiotics in the study. Increasing resistance was observed in the cases of several antibiotics. The results highlight the importance of testing Mycoplasma species for antibiotic susceptibility before therapy.

In vitroEvaluation of Various Antimicrobials against FieldMycoplasma gallisepticumandMycoplasma synoviaeIsolates in Egypt

“…Veterinarians usually ignore the side effects of synthetic antibiotics while recommending the therapy for avian mycoplasmosis. Moreover, the development of resistance to currently used drugs [1,6] incorporating with their high costs increased emphasis on the use of new and alternative antimicrobials.…”

Antimicrobial susceptibility profiles of porcine mycoplasmas isolated from samples collected in southern Europe

“…Freely accessible tylvalosin MIC values for mycoplasmas are limited up to date to M. hyopneumoniae, M. hyorhinis, M. synoviae, Mycoplasma sp. 1220 and M. gallisepticum [17][18][19][20]. However, the MIC values observed in our work for this antimicrobial against M. hyorhinis and M. hyosynoviae, and the in vitro [17] and in vivo efficacy [21] demonstrated for the treatment of M. hyopneumoniae infections, gives prominence to tylvalosin as one of the most effective macrolides against porcine mycoplasmosis, together with tulathromycin, a 15-membered macrolide.…”

“…Similar results were obtained by Tavío et al [17] after evaluating field strains of M. hyopneumoniae, as well as for the avian mycoplasma M. synoviae and Mycoplasma sp. 1220 [18,19]. Freely accessible tylvalosin MIC values for mycoplasmas are limited up to date to M. hyopneumoniae, M. hyorhinis, M. synoviae, Mycoplasma sp.…”

Emerging Antibiotic Resistance in Mycoplasma Microorganisms, Designing Effective and Novel Drugs / Therapeutic Targets: Current Knowledge and Futuristic Prospects

“…N o v e l , s p e c i f i c a n d e f f e c t i v e antimycoplasma antibiotics need to be explored for better therapeutic results. In vitro testing 54 , alternating antibiotics 81,84,85 , long acting antibiotics 86 , novel macrolide, tetracyclines, aminoglycosides or quinolones 81,84 , pleuro-mutilin antibiotics like valnemulin and tiamulin 87,88 and advanced antimicrobials 89,90 are the future prospects with possible promising results for making antibiotic therapy of mycoplasma safe and effective and combating antibiotic resistance 44 .…”