Challenges in the Use of Compact Disc-Based Centrifugal Microfluidics for Healthcare Diagnostics at the Extreme Point of Care

Gilmore, Jordon; Islam, Monsur; Martínez-Duarte, Rodrigo

doi:10.3390/mi7040052

Cited by 32 publications

(24 citation statements)

References 90 publications

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“…A notable subset of the microfluidic system types is centrifugal, or lab-on-a-disc, devices, first developed in the late 1990s [24]. The simplicity of operation, the lack of complicated external instrumentation for sample transport (e.g., pumps), the ability to easily eliminate bubbles, and the inherent capability of density-based separation make centrifugal systems uniquely suited for diagnostics at the extreme point-of-care [25,26]. Using basic rotary control, simple microfluidic features can provide a multitude of unit operations, such as valving, metering, and mixing, making it easy to achieve scalable parallelization and integration for an array of samples [27,28,29].…”

Section: Introductionmentioning

confidence: 99%

Rapid, Portable, Multiplexed Detection of Bacterial Pathogens Directly from Clinical Sample Matrices

et al. 2016

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Enteric and diarrheal diseases are a major cause of childhood illness and death in countries with developing economies. Each year, more than half of a million children under the age of five die from these diseases. We have developed a portable, microfluidic platform capable of simultaneous, multiplexed detection of several of the bacterial pathogens that cause these diseases. This platform can perform fast, sensitive immunoassays directly from relevant, complex clinical matrices such as stool without extensive sample cleanup or preparation. Using only 1 µL of sample per assay, we demonstrate simultaneous multiplexed detection of four bacterial pathogens implicated in diarrheal and enteric diseases in less than 20 min.

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Section: Introductionmentioning

confidence: 99%

Rapid, Portable, Multiplexed Detection of Bacterial Pathogens Directly from Clinical Sample Matrices

et al. 2016

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“…A number of studies have shown the possibility of being able to modify the exposed metal layer using readily available office printers [7,10,76] or similar techniques [73] or chemically to form self-assemble monolayers [32] of for the addition for functional groups [33]. Beyond the scope of this review, reports have shown the possibility of utilising CDs and DVDs for both sample preparation as well as quantification using approaches such as surface plasma resonance [89], centrifugal microfluidics or so called 'lab-on-a-disc' devices [90][91][92][93][94][95], DNA microarrays [96]. Their application in the exploitation of solar energy [97] and the development of batteries [98] has also been reported.…”

Section: Discussionmentioning

confidence: 99%

Cheap and disposable gold and silver electrodes: Trends in the application of compact discs and digital versatile discs for electroanalytical chemistry

Honeychurch

2017

TrAC Trends in Analytical Chemistry

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Highlights Compact discs and digital versatile discs can be readily used to give cheap reproducible Au and Ag electrodes. Their fabrication and use in electroanalytical chemistry is reviewed. Applications, performance characteristics and construction details are reviewed. AbstractIncreasingly more reports have focused on the use of digital versatile discs (DVDs) and compact discs (CDs) for the fabrication of electrodes. The majority of discs use Al to reflect the laser. However, a notable percentage utilise Au or Ag. This layer can be mechanically or chemically exposed allowing for the economic fabrication of 2 otherwise expensive Ag and Au electrodes. Cleaning steps are not required due to the layer's mirror like surface. Mass production of these discs means they are available, reproducible and disposable. Modifications can be made by laser, inkjet printing, etching or xurography. Self-assembled monolayers and the attachment of functional groups can also be made. This review (98 references and 5 figures) is divided into four sections. The first describes the development of these electrodes and their fabrication and modification. The next three sections focused on applications for the determination of metal ions, biomedical and environmental analysis.

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“…We have developed an automated-image-analysis smartphone application using machine learning to make assay readout more user-friendly,robust, and free of potential user bias.A tt he current stage,m ultiple hands-on steps are still needed to carry out the incubation and washing steps in microbubbling assays.F urther integration with automated systems,such as autonomous capillary microfluidic systems [31] disk-like microfluidic systems, [32] and programmable electrowetting-based droplet-mixing systems, [33] would simplify the microbubbling assay,m aking it ready for POC applications. We have developed an automated-image-analysis smartphone application using machine learning to make assay readout more user-friendly,robust, and free of potential user bias.A tt he current stage,m ultiple hands-on steps are still needed to carry out the incubation and washing steps in microbubbling assays.F urther integration with automated systems,such as autonomous capillary microfluidic systems [31] disk-like microfluidic systems, [32] and programmable electrowetting-based droplet-mixing systems, [33] would simplify the microbubbling assay,m aking it ready for POC applications.…”

Section: Forschungsartikelmentioning

confidence: 99%

Quantitation of Femtomolar‐Level Protein Biomarkers Using a Simple Microbubbling Digital Assay and Bright‐Field Smartphone Imaging

et al. 2019

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Quantitating ultra-lowc oncentrations of protein biomarkers is critical for early disease diagnosis and treatment. However,most current point-of-care (POC) assays are limited in sensitivity.Herein, we introduce an ultra-sensitive and facile microbubbling assayf or the quantification of protein biomarkers with ad igital-readout method that requires only as martphone camera. We used machine learning to develop as martphone application for automated image analysis to facilitate accurate and robust counting.U sing this method, post-prostatectomy surveillance of prostate specific antigen (PSA) can be achieved with adetection limit (LOD) of 2.1 fm (0.060 pg mL À1 ), and early pregnancy detection using bhCG can be achieved with aof0.034 mIU mL À1 (2.84 pg mL À1 ). This work provides the proof-of-principle of the microbubbling assayw ith ad igital readout as an ultra-sensitive technology with minimal requirement for power and accessories,facilitating future POC applications.

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Challenges in the Use of Compact Disc-Based Centrifugal Microfluidics for Healthcare Diagnostics at the Extreme Point of Care

Cited by 32 publications

References 90 publications

Rapid, Portable, Multiplexed Detection of Bacterial Pathogens Directly from Clinical Sample Matrices

Rapid, Portable, Multiplexed Detection of Bacterial Pathogens Directly from Clinical Sample Matrices

Cheap and disposable gold and silver electrodes: Trends in the application of compact discs and digital versatile discs for electroanalytical chemistry

Quantitation of Femtomolar‐Level Protein Biomarkers Using a Simple Microbubbling Digital Assay and Bright‐Field Smartphone Imaging

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