Challenges and Perspectives in the Study of Self-Incompatibility in Orchids

Zhang, Xiaojing; Jia, Yin; Liu, Yang; Chen, Duanfen; Luo, Yan; Niu, Shan-Ce

doi:10.3390/ijms222312901

Cited by 6 publications

(3 citation statements)

References 39 publications

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“…Beyond dioecy, auxin may play roles in other SI species. The pollen of the Orchidaceae contains large amounts of auxin and treatment with auxin can result in SC or partially SC plants [ 84 ]. Though the role of the YUCCA family has not been investigated in the Orchidaceae , the presence of high auxin levels in pollen and the YUCCA family’s involvement in auxin synthesis suggests that a YUCCA gene might be involved.…”

Section: Discussionmentioning

confidence: 99%

The S-Gene YUC6 Pleiotropically Determines Male Mating Type and Pollen Size in Heterostylous Turnera (Passifloraceae): A Novel Neofunctionalization of the YUCCA Gene Family

Henning

Shore

McCubbin

2022

Plants

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In heterostylous, self-incompatible Turnera species, a member of the YUCCA gene family, YUC6, resides at the S-locus and has been hypothesized to determine the male mating type. YUCCA gene family members synthesize the auxin, indole-3-acetic acid, via a two-step process involving the TAA gene family. Consequently, it has been speculated that differences in auxin concentration in developing anthers are the biochemical basis underlying the male mating type. Here, we provide empirical evidence that supports this hypothesis. Using a transgenic knockdown approach, we show that YUC6 acts pleiotropically to control both the male physiological mating type and pollen size, but not the filament length dimorphism associated with heterostyly in Turnera. Using qPCR to assess YUC6 expression in different transgenic lines, we demonstrate that the level of YUC6 knockdown correlates with the degree of change observed in the male mating type. Further assessment of YUC6 expression through anther development, in the knockdown lines, suggests that the male mating type is irreversibly determined during a specific developmental window prior to microsporogenesis, which is consistent with the genetically sporophytic nature of this self-incompatibility system. These results represent the first gene controlling male mating type to be characterized in any species with heterostyly.

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Section: Discussionmentioning

confidence: 99%

The S-Gene YUC6 Pleiotropically Determines Male Mating Type and Pollen Size in Heterostylous Turnera (Passifloraceae): A Novel Neofunctionalization of the YUCCA Gene Family

Henning

Shore

McCubbin

2022

Plants

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“…With SI, inbreeding is effectively avoided, and the probability of extinction is greatly reduced. At present, most studies on SI have focused on the Cruciferae , Solanaceae , Rosaceae , Plantaginaceae and Papaveraceae plant species [ 1 ]. Studies on SI in Rutaceae plants are quite rare.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis of the RNase T2 Family and Identification of Interacting Proteins of Four ClS-RNase Genes in ‘XiangShui’ Lemon

Zhu

Lin

et al. 2022

IJMS

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S-RNase plays vital roles in the process of self-incompatibility (SI) in Rutaceae plants. Data have shown that the rejection phenomenon during self-pollination is due to the degradation of pollen tube RNA by S-RNase. The cytoskeleton microfilaments of pollen tubes are destroyed, and other components cannot extend downwards from the stigma and, ultimately, cannot reach the ovary to complete fertilisation. In this study, four S-RNase gene sequences were identified from the ‘XiangShui’ lemon genome and ubiquitome. Sequence analysis revealed that the conserved RNase T2 domains within S-RNases in ‘XiangShui’ lemon are the same as those within other species. Expression pattern analysis revealed that S3-RNase and S4-RNase are specifically expressed in the pistils, and spatiotemporal expression analysis showed that the S3-RNase expression levels in the stigmas, styles and ovaries were significantly higher after self-pollination than after cross-pollination. Subcellular localisation analysis showed that the S1-RNase, S2-RNase, S3-RNase and S4-RNase were found to be expressed in the nucleus according to laser confocal microscopy. In addition, yeast two-hybrid (Y2H) assays showed that S3-RNase interacted with F-box, Bifunctional fucokinase/fucose pyrophosphorylase (FKGP), aspartic proteinase A1, RRP46, pectinesterase/pectinesterase inhibitor 51 (PME51), phospholipid:diacylglycerol acyltransferase 1 (PDAT1), gibberellin receptor GID1B, GDT1-like protein 4, putative invertase inhibitor, tRNA ligase, PAP15, PAE8, TIM14-2, PGIP1 and p24beta2. Moreover, S3-RNase interacted with TOPP4. Therefore, S3-RNase may play an important role in the SI of ‘XiangShui’ lemon.

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“…In Orchidaceae, SI is mostly found in the subfamily Epidendroideae, and the SI phenotypes are diverse, even within the same genus. Hormones, i.e., auxin and ethylene, and new male and female determinants could be involved in the SI response [ 8 ].…”

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confidence: 99%