Chain stiffness and extension of chitosans and periodate oxidised chitosans studied by size-exclusion chromatography combined with light scattering and viscosity detectors

Characterization and Properties of Hydrogels Made from Neutral Soluble Chitosans

“…By use of Size Exclusion Chromatography-Multi Angle Laser Light Scattering (SEC-MALLS) the number and weight average molecular weights were determined according to [26]. The weight average molecular weight, , and the number average molecular weight, , together with the polydispersity index, / , are given in Table 1.…”

In situ preparation of high relaxivity iron oxide nanoparticles by coating with chitosan: A potential MRI contrast agent useful for cell tracking

“…Chitosan has intrinsic persistence lengths in the range 5.1-7.6 nm [46] that may influence its aggregation properties after being coated on iron oxide nanoparticles. The length of the chitosan polymer chain is sufficient to balance the magnetic attractions and the van der Waals attractive force of the dextran polymer chain.…”

Influence of the Chemical Structure and Physicochemical Properties of Chitin- and Chitosan-Based Materials on Their Biomedical Activity

“…• SEC-RI (pullulan calibration) (Hasegawa et al, 1994;Knill et al, 2005;Lin & Lin, 2003) • SEC-LALS (Kubota et al, 2000;Ottøy et al, 1996Ottøy et al, , 1995 • SEC-MALS (Tømmeraas et al, 2002;Sorlier et al, 2001;Sorlier, 2002;Berth et al, 1998;Lamarque et al, 2004Lamarque et al, , 2005Schatz et al, 2003;Brugnerotto et al, 2001) • SEC-LALS-Vis (Weinhold et al, 2009) • SEC-MALS-Vis (Christensen et al, 2008) Since the use of size-exclusion chromatography is commonly agreed upon, more controversy is present about surrounds the different detectors used online for chromatography. Nowadays, all the old bench methods can be applied to a chromatography setup such as a refractive index detector (RI), viscosity detector (Vis) and light-scattering detectors (single-90°, low-angle and 90° (LALS), or multi-angle with 3, 5, or up to 18 angles (MALS).…”

“…The diagram (Fig. 2) presents values obtained at 436 nm (Wang et al 1991) and values obtained at 633 nm (Terbojevich et al, 1991;Christensen et al, 2008;Kasaai et al, 2000;Chen & Tsaih, 1998;Berth et al, 1998;Schatz et al, 2003) for different chitosan preparations with changing FA(DA). The reason for this change is that dn/dc enters the molecular weight calculation quadratically.…”

“…The reason for this change is that dn/dc enters the molecular weight calculation quadratically. Using, for example, dn/dc = 0.142 (Christensen et al, 2008) for calculating the M W of a high molecular weight chitosan sample, we obtain a molecular weight of 1098 kg/mol. Changing the dn/dc value to 0.208 (Berth et al, 1998) for the same chromatography data, the M W decreases to 750 kg/mol.…”