cH-Schwellen der Uraninf�rbbarkeit des Protoplasmas pflanzlicher Zellen

Diannelidis, Themistokles; Tsekos, I.

doi:10.1007/bf01252534

Cited by 5 publications

(3 citation statements)

References 15 publications

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“…The vacuole stained in this way displays an absorption maximum at 490 nm. The decrease in absorption, however, of the right side of the curve is more abrupt than in the absorption curve derived from natural displacement of the dye ( (Table 1) is in full agreement only with the behavior of an aqueous solution at pH 6.0-11.0 (Tsekos 1968, Diannelidis andTsekos 1968).…”

Section: Mode! Experimentssupporting

confidence: 54%

Microspectrophotometric Analysis of Accumulation of the Fluorones K-Fluorescein, Rose Bengal and Phloxine Red in Living Plant Cells

Tsekos

Platis

Tsekos

1997

Biotechnic & Histochemistry

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Spectrophotometric investigations of dye solutions in different media and of living stained cells from the upper epidermis of the scaleleaf of Allium cepa were carried out with the dyes K-fluorescein, rose Bengal and phloxine red to elucidate the mechanism of the accumulation of these dyes in the cytoplasm, the nucleus and the cell sap. Thin layer chromatography and paper electrophoresis indicate that the K-fluorescein used here contains no detectable contaminants. Besides the main component, rose Bengal contains two components in small quantities with Rf values of 0.64 and 0.57, plus three more components in traces. Besides the two main components (Rf values of 0.83 and 0.73), phloxine red also contains five more components in traces. Electrophoretic investigations reveal that in aqueous solution the fluorones rose Bengal and phloxine red from pH 2.0-11 show a migration toward the anode. K-fluorescein from pH 2.9-10.4 shows a migration toward the anode, but at pH 1.9 a migration toward the cathode. By shaking aqueous solutions of K-fluorescein, rose Bengal and phloxine red at different pH values with different organic solvents, the above used stainings show different spectral absorption curves according to the polarity of the solvent. The position of the absorption maxima and the shape of the absorption curves of these three anionic dyes lead to the conclusion that the staining of the living cytoplasm and nucleus is due to ion accumulation by means of the "ion trap mechanism" within the aqueous phase of the cytoplasm (cytosol) and the nucleus. Adsorption of dye particles in the protein phase of the cytoplasm cannot be excluded. There seems to be a fundamental difference in the vital staining of the protoplasm by anionic and cationic dyes, the latter apparently accumulating as neutral dye molecules in the lipid phase of the protoplasm. The concentration of the dyes used in the living cytoplasm (cytosol) is approximately 0.2-0.05%. During natural and artificial displacement of K-fluorescein from the cytoplasm to the vacuole, it appears that accumulation of the dye within the vacuole is performed through an ion trap mechanism in the form of bivalent ions. Along with natural displacement, it is possible that ion accumulation also occurs in metabolic products.

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Section: Mode! Experimentssupporting

confidence: 54%

Microspectrophotometric Analysis of Accumulation of the Fluorones K-Fluorescein, Rose Bengal and Phloxine Red in Living Plant Cells

Tsekos

Platis

Tsekos

1997

Biotechnic & Histochemistry

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“…Das Cytoplasma der Rotalgen weist merkwürdige physiologische Eigenschaften auf (Höfler 1930(Höfler ,1936(Höfler ,1944(Höfler , 1956Diannelidis und Mitrakos 1959;Diannelidis und Tsekos 1968;Tsekos 1965Tsekos , 1968Tsekos, Haritonidis und Diannelidis 1972). Angeregt durch Untersuchungen über den Einfluß des pH, der Lichtintensität und Lichtqualität auf Karposporen-Keimlinge von Gracilaria confervoides (Mitrakos 1964) prüften wir den Einfluß unterschiedlicher Temperatur auf deren Wachstum.…”

unclassified

Der Einfluß der Temperatur auf das Wachstum von Karposporen-Keimlingen der Rhodophycee Gracilaria confervoides (L.) Grev.

Tsekos¹,

Karataglis²

1974

Botanica Marina

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“…Μπροστά από το κάθε είδος σημειώνεται ο κωδικός που δόθηκε για την στατιστική ανάλυση και χρησιμοποιείται στα αντίστοιχα σχήματα. Athanasiadis (1987), Ballesteros (1990c), Ben Maiz et al (1987), Ben Maiz et al (1988, Boudouresque & Perret-Boudouresque (1987), Boudouresque et al (1984), Cirik (1978, Delepine et al (1987), Diannelidis & Tsekos (1968), Diapoulis & Haritonidis (1987a,b), Feldmann (G. & J.) (1942), Gallardo et al (1993), Giaccone et al (1985), Guven & Oztig (1971), Menez & Mathieson (1981), Mayhoub (1976), Nizamuddin et al (1979), Ribera & Garreta (1984, Ribera et al (1992), Schnetter & Schnetter (1981), Tsekos (1965Tsekos ( , 1968Tsekos ( , 1970, , Tsekos et al (1982), Tsekos & Reiss (1992), Tsekos et al (1993), Verlaque (1987Verlaque ( , 1990.…”

Section: αλατότηταunclassified

Συστηματικη Βιονομικη Και Οικολογικη Μελετη Του Θαλασσιου Φυτοβενθους Της Νησου Μηλου (Κυκλαδες)

Λαζαριδου¹

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cH-Schwellen der Uraninf�rbbarkeit des Protoplasmas pflanzlicher Zellen

Cited by 5 publications

References 15 publications

Microspectrophotometric Analysis of Accumulation of the Fluorones K-Fluorescein, Rose Bengal and Phloxine Red in Living Plant Cells

Microspectrophotometric Analysis of Accumulation of the Fluorones K-Fluorescein, Rose Bengal and Phloxine Red in Living Plant Cells

Der Einfluß der Temperatur auf das Wachstum von Karposporen-Keimlingen der Rhodophycee Gracilaria confervoides (L.) Grev.

Συστηματικη Βιονομικη Και Οικολογικη Μελετη Του Θαλασσιου Φυτοβενθους Της Νησου Μηλου (Κυκλαδες)

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