cfxA expression in oral clinical Capnocytophaga isolates

Tamanai-Shacoori, Zohreh; Monfort, Clarisse; Oliviero, Nolwenn; Gautier, Philippe; Bonnaure‐Mallet, Martine; Jolivet‐Gougeon, Anne

doi:10.1016/j.anaerobe.2015.07.005

Cited by 7 publications

(6 citation statements)

References 29 publications

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“…The susceptibilities of the bla CfxA or bla CSP gene-harbouring Capnocytophaga spp. to β-lactam antibiotics were in good agreement with those previously reported [7, 21, 27], as shown in . The MIC values with regard to ceftazidime decreased when combined with clavulanic acid.…”

Section: Discussionsupporting

confidence: 92%

“…Variation in the MIC values among each β-lactamase-producing Capnocytophaga species could be influenced by various genetic events, as described by Tamanai-Shacoori et al . [27]. Cefoxitin and imipenem are considered to be potentially effective antimicrobials for the treatment of CfxA- or CSP-producing Capnocytophaga infections, but need to be validated in animal models.…”

Section: Discussionmentioning

confidence: 99%

“…had higher MIC 90 values with regard to cefepime and higher MIC 50 values with regard to amoxicillin than the bla CSP gene-harbouring Capnocytophaga spp. Variation in the MIC values among each β-lactamaseproducing Capnocytophaga species could be influenced by various genetic events, as described by Tamanai-Shacoori et al[27]. Cefoxitin and imipenem are considered to be potentially effective antimicrobials for the treatment of CfxA-or CSP-producing Capnocytophaga infections, but need to be validated in animal models.In conclusion, our findings revealed a high prevalence of the bla CSP gene in β-lactamaseproducing Capnocytophaga species in Japan.…”

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confidence: 99%

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Antibiotic resistance and the presence of bla CfxA and bla CSP genes in β-lactamase-producing clinical Capnocytophaga isolates from a university hospital in Japan

Matsumoto

Matsumoto-Matsubara

Horiuchi

et al. 2021

Journal of Medical Microbiology

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Introduction . Capnocytophaga species are common inhabitants of the oral cavity and can be responsible for systemic diseases in immunocompromised patients with granulocytopenia. Furthermore, it has been reported that some clinical isolates of Capnocytophaga species produce extended-spectrum β-lactamases (ESBLs). Gap statement. Information is lacking about the types of β-lactamase genes possessed by Capnocytophaga spp. and the antimicrobial susceptibility of Capnocytophaga spp. possessing each β-lactamase gene. Aim. The aim of this study was to investigate the presence of β-lactamase genes in clinical strains of β-lactamase-producing Capnocytophaga species isolated from clinical samples acquired at Shinshu University Hospital and examine the antimicrobial susceptibility of those strains. Methodology. The β-lactamase-producing Capnocytophaga species (n=49) were obtained from clinical specimens. PCR assays were used to detect bla CfxA, bla CSP, bla TEM, bla CepA/CblA and transposon Tn4555 genes. Southern hybridization assays were used to detect bla CfxA and bla CSP. The minimum inhibitory concentration of some β-lactams was determined using the E-test method. Results. PCR analysis indicated that the bla CfxA gene was present in 15 (30.6 %) and the bla CSP gene in 35 (69.3 %) of the 49 Capnocytophaga strains investigated, . Both bla CfxA and bla CSP genes were detected in a Capnocytophaga gingivalis strain. The PCR results were confirmed by Southern hybridization assays. Transposon Tn4555 was only detected in Capnocytophaga spp. harbouring the bla CfxA gene. All the β-lactamase-producing Capnocytophaga isolates were susceptible to ceftazidime–clavulanic acid, cefoxitin and imipenem. In contrast, most of the isolates were resistant to amoxicillin. Conclusions. The clinical isolates of Capnocytophaga spp. showed a high prevalence of the bla CSP gene in Japan. The presence of the bla CSP gene was distributed in Capnocytophaga sputigena as well as other Capnocytophaga spp. These results seem to suggest the dissemination of bla CfxA and bla CSP β-lactamase genes among Capnocytophaga species.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Antibiotic resistance and the presence of bla CfxA and bla CSP genes in β-lactamase-producing clinical Capnocytophaga isolates from a university hospital in Japan

Matsumoto

Matsumoto-Matsubara

Horiuchi

et al. 2021

Journal of Medical Microbiology

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“…The breakdown of the different CfxA beta-lactamases (Figure 7) revealed a statistically significant decrease in CfxA2 on Prevotella (p = 0.0469) after ceftiofur treatment. CfxA beta-lactamases are genetically closely related and differ only by point mutations [70]. Several studies have determined different kinetic parameters for the variants of these CfxA genes against a number of beta-lactam antimicrobials [71,72].…”

Section: Impact Of Ceftiofur On the Gut Resistome Of Pigsmentioning

confidence: 99%

Intestinal Exposure to Ceftiofur and Cefquinome after Intramuscular Treatment and the Impact of Ceftiofur on the Pig Fecal Microbiome and Resistome

et al. 2022

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Optimization of antimicrobial treatment during a bacterial infection in livestock requires in-depth knowledge of the impact of antimicrobial therapy on the pathogen and commensal microbiota. Once administered antimicrobials and/or their metabolites are excreted either by the kidneys through urine and/or by the intestinal tract through feces, causing antimicrobial pressure and possibly the emergence of resistance in the gastro-intestinal tract. So far, the excretion of ceftiofur and cefquinome in the intestinal tract of pigs has not been described. The objective of this study was to investigate the excretion of ceftiofur and cefquinome in the different segments of the gut and feces after intramuscular administration. Therefore, 16 pigs were treated either with ceftiofur (n = 8) or cefquinome (n = 8), and feces were collected during the entire treatment period. The presence of ceftiofur and desfuroylceftiofuracetamide or cefquinome were quantified via liquid chromatography–tandem mass spectrometry. At the end of the treatment, pigs were euthanized, and samples from the duodenum, jejunum, ileum and cecum were analyzed. In feces, no active antimicrobial residues could be measured, except for one ceftiofur-treated pig. In the gut segments, the concentration of both antimicrobials increased from duodenum toward the ileum, with a maximum in the ileum (187.8 ± 101.7 ng·g−1 ceftiofur-related residues, 57.8 ± 37.5 ng·g−1 cefquinome) and sharply decreased in the cecum (below the limit of quantification for ceftiofur-related residues, 6.4 ± 4.2 ng·g−1 cefquinome). Additionally, long-read Nanopore sequencing and targeted quantitative polymerase chain reaction (qPCR) were performed in an attempt to clarify the discrepancy in fecal excretion of ceftiofur-related residues between pigs. In general, there was an increase in Prevotella, Bacteroides and Faecalibacterium and a decrease in Escherichia and Clostridium after ceftiofur administration (q-value < 0.05). The sequencing and qPCR could not provide an explanation for the unexpected excretion of ceftiofur-related residues in one pig out of eight. Overall, this study provides valuable information on the gut excretion of parenteral administered ceftiofur and cefquinome.

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“…. ), the expression of beta-lactamase may differ according to major substitutions in the cfxA gene sequence or to the influence of the genetic environment of cfxA [30]. This molecular part has not been evaluated in this study.…”

Section: Discussionmentioning

confidence: 99%

Prevotella melaninogenica, a Sentinel Species of Antibiotic Resistance in Cystic Fibrosis Respiratory Niche?

et al. 2021

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The importance and abundance of strict anaerobic bacteria in the respiratory microbiota of people with cystic fibrosis (PWCF) is now established through studies based on high-throughput sequencing or extended-culture methods. In CF respiratory niche, one of the most prevalent anaerobic genera is Prevotella, and particularly the species Prevotella melaninogenica. The objective of this study was to evaluate the antibiotic susceptibility of this anaerobic species. Fifty isolates of P. melaninogenica cultured from sputum of 50 PWCF have been included. Antibiotic susceptibility testing was performed using the agar diffusion method. All isolates were susceptible to the following antibiotics: amoxicillin/clavulanic acid, piperacillin/tazobactam, imipenem and metronidazole. A total of 96% of the isolates (48/50) were resistant to amoxicillin (indicating beta-lactamase production), 34% to clindamycin (17/50) and 24% to moxifloxacin (12/50). Moreover, 10% (5/50) were multidrug-resistant. A significant and positive correlation was found between clindamycin resistance and chronic azithromycin administration. This preliminary study on a predominant species of the lung “anaerobiome” shows high percentages of resistance, potentially exacerbated by the initiation of long-term antibiotic therapy in PWCF. The anaerobic resistome characterization, focusing on species rather than genera, is needed in the future to better prevent the emergence of resistance within lung microbiota.

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cfxA expression in oral clinical Capnocytophaga isolates

Cited by 7 publications

References 29 publications

Antibiotic resistance and the presence of bla CfxA and bla CSP genes in β-lactamase-producing clinical Capnocytophaga isolates from a university hospital in Japan

Antibiotic resistance and the presence of bla CfxA and bla CSP genes in β-lactamase-producing clinical Capnocytophaga isolates from a university hospital in Japan

Intestinal Exposure to Ceftiofur and Cefquinome after Intramuscular Treatment and the Impact of Ceftiofur on the Pig Fecal Microbiome and Resistome

Prevotella melaninogenica, a Sentinel Species of Antibiotic Resistance in Cystic Fibrosis Respiratory Niche?

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