Cervicare™ induces apoptosis in HeLa and CaSki cells through ROS production and loss of mitochondrial membrane potential

Amini, Neda; Majid, Fadzilah Adibah Abdul; Marvibaigi, Mohsen; Supriyanto, Eko; Jaganathan, Saravana Kumar; Soon, Wong Tet; Nasiri, Rozita; Hamzehalipour, Javad

doi:10.1039/c5ra25654b

Cited by 13 publications

(7 citation statements)

References 57 publications

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“…Since AO dye cannot differentiate between the cells, a mixture of AO and EB was used. EB solely penetrates dead cells, since their membrane has lost integrity, thus staining the nuclei red [79]. Overall, there was a good correlation between the MTT and the AO/EB apoptosis assays.…”

Section: Discussionmentioning

confidence: 75%

Histidine-Tagged Folate-Targeted Gold Nanoparticles for Enhanced Transgene Expression in Breast Cancer Cells In Vitro

et al. 2021

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Nanotechnology has emerged as a promising treatment strategy in gene therapy, especially against diseases such as cancer. Gold nanoparticles (AuNPs) are regarded as favorable gene delivery vehicles due to their low toxicity, ease of synthesis and ability to be functionalized. This study aimed to prepare functionalized AuNPs (FAuNPs) and evaluate their folate-targeted and nontargeted pCMV-Luc-DNA delivery in breast cancer cells in vitro. CS was added to induce stability and positive charges to the AuNPs (Au-CS), histidine (Au-CS-His) to enhance endosomal escape and folic acid for folate-receptor targeting (Au-CS-FA-His). The FAuNP:pDNA nanocomplexes possessed favorable sizes (<135 nm) and zeta potentials (<−20 mV), strong compaction efficiency and were capable of pDNA protection against nuclease degradation. These nanocomplexes showed minimal cytotoxicity (>73% cell viability) and enhanced transgene activity. The influence of His was notable in the HER2 overexpressing SKBR3 cells, which produced higher gene expression. Furthermore, the FA-targeted nanocomplexes enhanced receptor-mediated endocytosis, especially in MCF-7 cells, as confirmed by the receptor competition assay. While the role of His may need further optimization, the results achieved suggest that these FAuNPs may be suitable gene delivery vehicles for breast cancer therapeutics.

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Section: Discussionmentioning

confidence: 75%

Histidine-Tagged Folate-Targeted Gold Nanoparticles for Enhanced Transgene Expression in Breast Cancer Cells In Vitro

et al. 2021

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“…The morphological apoptosis of cells aer treatment with neat Br and SPIONs-Br-FA was observed using an inverted microscope according to the standard protocol. 64 A solution of 1 Â 10 5 cells per ml was seeded in 6-well plates and the cells were treated for 24 h with neat Br (at the IC 50 value) and SPIONs-Br-FA (at the IC 50 value of neat Br), with a 70% level of conuence achieved. The negative control was the untreated cells, while cisplatin was used as the positive apoptosis control.…”

Section: Morphological Assessment By Phase-contrast Inverted Microscopymentioning

confidence: 99%

“…In order to measure the ability of the FAR+ cells treated with neat Br (at the IC 50 value) and SPIONs-Br-FA (at the IC 50 value of neat Br), a scratch assay was performed according to Amini et al, 64 with slight modications. Briey, in a 6-well plate, 3 Â 10 5 cells per well were seeded and incubated to obtain a conuent monolayer.…”

Section: Scratch Motility Assaymentioning

confidence: 99%

Targeted delivery of bromelain using dual mode nanoparticles: synthesis, physicochemical characterization, in vitro and in vivo evaluation

et al. 2017

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“…It is significantly important to evaluate these different mitochondrial behaviors through monitoring the corresponding MMP situations. Here the decreased and vanished MMP states of MCF‐7 or HeLa cells were realized by their incubation with CCCP, a MMP uncoupler, for 10 and 20 min, respectively (Figures S8 and S9, Supporting Information). Meanwhile, the mitochondria maintain a normal MMP status without CCCP treatment.…”

Section: Resultsmentioning

confidence: 99%

Spatiotemporally Tracking the Programmable Mitochondrial Membrane Potential Evolutions by a Robust Molecular Rotor

Tan

Chen

et al. 2019

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potential (MMP) is a vital parameter for reflecting the real-time status of mitochondrial function, and is thus closely correlated to cellular activities and functions. [3] Normal MMP maintains the efficient ATP synthesis and an appropriate proton gradient across the lipid bilayer, which plays a vital role in cell division and apoptosis. On the contrary, a minute MMP change could largely affect the mitochondrial functions. The aberrant MMP change has been frequently recognized as an early signal event for irreversible cell apoptosis, making the investigation of MMP variation a research focus in biological and biomedical researches. Moreover, the constant MMP fluctuations of intracellular physiological processes bring obstacles to the in situ MMP detection in living cells. Therefore, it is highly desirable and challenging to explore more robust and versatile approaches for monitoring the intracellular MMP fluctuations in realtime at a single-cell level.Fluorescent probes have attracted substantial attentions in biological researches for its high sensitivity and noninvasion capacity in combination with the real-time fluorescence imaging technique. [4] To date, plenty of fluorescence probes have been developed for monitoring MMP variations. [5] These probes are mainly based on the signal transduction between mitochondria and cytosol, such as rhodamine 123 (Rh 123), [5a] yet it is difficult to judge the decreased and vanished status of MMP just by fluorescence distribution owing to the ambiguous boundary between mitochondria and the surrounding cytosol. An alternative kind of fluorescent dichromatic probe JC-1, [6] with a moderate anti-interference feature, was then developed for discriminating these different MMP states based on an intramitochondria signal transduction mechanism, yet the inconvenient usage, low sensitivity, and limited photostability need to be solved before their extensive applications can be envisaged. Thus, it is essential to develop a robust and sensitive fluorescent probe for conveniently and accurately evaluating minute MMP fluctuations, such as normal, decreased, and vanished status.The ambiguous biointerface of mitochondria inspired us to utilize an alternative cellular compartment with distinct boundary for highly sensitive investigation of MMP variations. Considering that nucleus is spatially independent of cytosol, and thus there exists a distinct boundary between mitochondria Mitochondrial membrane potential (MMP) represents an essential parameter of cellular activities, and even a minute MMP variation could significantly affect the biological functions of living organisms. Thus, convenient and accurate MMP detection is highly desirable since conventional MMP probes are always constrained by photobleaching, inconvenience, and irreversibility. Herein, a spatial-dependent fluorescent molecular rotor Mito-Cy is introduced for efficiently tracking the varied MMP status through its restricted intramolecular rotation in mitochondria and nucleus compartments. Based on a systematic investig...

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Cervicare™ induces apoptosis in HeLa and CaSki cells through ROS production and loss of mitochondrial membrane potential

Abstract: The effect of the ethanol and aqueous extracts of Cervicare™, a poly-herbal preparation comprised of the combination of 6 plants, on cell proliferation and apoptosis using cervical cancer HeLa and CaSki cells was investigated for the first time in the present study.

Cited by 13 publications

References 57 publications

Histidine-Tagged Folate-Targeted Gold Nanoparticles for Enhanced Transgene Expression in Breast Cancer Cells In Vitro

Histidine-Tagged Folate-Targeted Gold Nanoparticles for Enhanced Transgene Expression in Breast Cancer Cells In Vitro

Targeted delivery of bromelain using dual mode nanoparticles: synthesis, physicochemical characterization, in vitro and in vivo evaluation

Spatiotemporally Tracking the Programmable Mitochondrial Membrane Potential Evolutions by a Robust Molecular Rotor

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