Targeted delivery of bromelain using dual mode nanoparticles: synthesis, physicochemical characterization, in vitro and in vivo evaluation

Nasiri, Rozita; Almaki, Javad Hamzehalipour; Nasiri, Mahtab; Irfan, Muhammad; Majid, Fadzilah Adibah Abdul; Nodeh, Hamid Rashidi; Hasham, Rosnani

doi:10.1039/c7ra06389j

Cited by 27 publications

(31 citation statements)

References 97 publications

(116 reference statements)

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“…Bromelain at IC 50 concentration reduced the size and number of colonies of HeLa, MDA-MB-231, and 4T1 cells. [8] Our results are in agreement with those of a previous study. The number of colonies of all the three human cancer cells decreased after treatment with bromelain at IC 50 concentration [Figures 3-5].…”

Section: Discussionsupporting

confidence: 93%

“…[12131415] Bromelain induced the cytotoxic effects on murine lung carcinoma, mammary adenocarcinoma, leukemia, lymphoma, sarcoma, melanoma and ascetic tumor cell lines, human gastrointestinal carcinoma cells (MKN45, KATO-III, HT29-5F12, and HT29-5M21), glioma, breast cancer, epidermoid carcinoma, melanoma, malignant peritoneal mesothelioma,[16] human skin fibroblasts 1184, human cervical cancer cells (HeLa), human breast cancer cells (MDA-MB-468 and MDA-MB-231), and murine breast cancer cells (4T1). [813141617]…”

Section: Discussionmentioning

confidence: 99%

“…A study by Nasiri et al . [8] showed that bromelain as a single or combination treatment agent had clonogenic inhibitory effect on human cervical cancer cell (HeLa), human breast cancer (MDA-MB-231), and murine breast cancer (4T1). In addition, they found that the survival rate decreased as a consequence of pretreatment with bromelain.…”

Section: Discussionmentioning

confidence: 99%

“…[7] The anticancer effect of bromelain as a single therapy modality or in combination with other chemotherapeutic drugs has been shown in some human cancer cells. [8]…”

Section: Introductionmentioning

confidence: 99%

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Bromelain inhibitory effect on colony formation: An In vitro Study on human AGS, PC3, and MCF7 cancer cells

Raeisi¹,

Raeisi²,

Heidarian³

et al. 2019

J Med Signals Sens

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Bromelain is dotted with anticancer properties on various cancer cell lines. Anticancer pathways of bromelain, as well related intervening signalization are under investigation. Investigating the inhibitory potential of bromelain on AGS, PC3, and MCF7 cells proliferation and colony formation. The bromelain inhibitory potential on AGS, PC3, and MCF7 cells proliferation at various bromelain concentrations was assessed by MTT; thereby, bromelain potency on colony formation impediment was evaluated using clonogenic assays at determined 50% inhibitory concentrations (IC50) on four different cell densities (10, 50, 100, and 200 cells per well). Bromelain inhibits AGS, PC3, and MCF7 cells proliferation in such a dose-dependent manner. Determined IC50 to AGS, PC3, and MCF7 cells were 65, 60 and 65μg/ml respectively. At IC50, bromelain significantly suppressed the AGS, PC3, and MCF7 cells colony formation at four treated densities (10, 50, 100 and 200 cells per well). Plating efficiency percentage and cell surviving fraction were decreased after bromelain treatment to AGS, PC3, and MCF7 human cancer cells as a function of initial cell density. The 50, 50 or 100, and 10 or 50 cells per well were considered to be optimum number of initial cell density for AGS, PC3, and MCF7 cells. Cell proliferative and colony formation inhibition are two pathways to in vitro bromelain anticancer effects. The current study displayed a dose-dependent inhibitory effect of bromelain, as well impeding colony formation AGS, PC3, and MCF7 human cancer cells.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…[7] The anticancer effect of bromelain as a single therapy modality or in combination with other chemotherapeutic drugs has been shown in some human cancer cells. [8]…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Bromelain inhibitory effect on colony formation: An In vitro Study on human AGS, PC3, and MCF7 cancer cells

Raeisi¹,

Raeisi²,

Heidarian³

et al. 2019

J Med Signals Sens

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“…Curcumin solution achieved the highest concentration (1.61 ± 0.25 μg/10 5 cells) in 30 min whereas drug level (2.64 ± 0.21 μg/10 5 cells) in optimized formulation treated cells was on the peak after 2 h as shown in Figure 6(c). The drug level in native curcumin‐treated cells reduced rapidly with time due to its rapid distribution, metabolism and degradation rather than internalization by the cells . Furthermore, a significantly high concentration of curcumin in optimized formulation treated cells at each time point revealed higher uptake, improved stability of curcumin and sustained release potential of the nanoparticulate formulation…”

Section: Dissolution Studymentioning

confidence: 99%

Rationally designed curcumin laden glycopolymeric nanoparticles: Implications on cellular uptake and anticancer efficacy

Rao

Sharma

Palodkar

et al. 2020

J of Applied Polymer Sci

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Overexpression of glucose transport proteins (GLUTs) plays a pivotal role in the survival of cancer cells. Hence, targeting GLUTs receptors using glucose‐based polymers can fill up the lacuna of cancer treatment by confining the dissemination and accumulation of chemotherapeutic drugs on cancer cells. The present study addressed the preparation of glycohomopolymer (PMG), PEG‐based di‐ (PEG‐b‐PMG) and tri‐block (PMG‐b‐PEG‐b‐PMG) polymers using atom transfer radical polymerization and their potential in the development of novel nanoparticulate drug delivery system. Curcumin‐loaded glycopolymer nanoparticles were fabricated by nanoprecipitation method and investigated for various physicochemical parameters such as particle size, zeta potential, polydispersity index, drug loading, morphology, and dissolution profile. Homoglycopolymer nanoparticles exhibited lower average particle size (240.16 ± 21.41 nm), higher zeta potential (−28.72 ± 4.25 mV), and entrapment efficiency (74.61 ± 5.03%) compared to their block copolymer counterparts. Optimized formulation exhibited diffusion and dissolution‐controlled drug release behavior. In vitro cell line studies demonstrated significantly superior cytotoxicity, clonogenic inhibitory and cellular uptake potential in MCF‐7 cells besides receptor recognizing property of optimized curcumin nanoparticulate formulation compared to free curcumin. These findings elucidate that curcumin homoglucopyranoside nanocarriers can be a promising drug delivery option for effective management of breast cancer. © 2020 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48954.

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Mucoadhesive Cationic Bromelain Laden Nanocarriers Restore Patency of Airway Hyperresponsive Remodeling via Nasal Route

Sharma

Gupta

2023

Advanced Therapeutics

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Bromelain, a complex mixture of proteolytic cysteine proteases has shown positive attributes in management of allergen induced inflammatory conditions. However, efficient use of bromelain in clinics is restricted by bromelain instability at storage conditions and on oral use. Therefore, the goal of present investigation is to encapsulate bromelain in rationally tailored mucoadhesive polymeric nanocarriers for nasal delivery to refurbish its anti‐asthmatic activity and improve bioavailability. Bromelain laden Eudragid RL 100 nanocarriers (Br‐EuNCs) are engineered via double emulsion solvent evaporation method and optimized to amplify bromelain encapsulation within nanosize range (259.73 ± 16.51 nm). Dissolution study reveals prolonged bromelain release behavior (24 h) whereas higher mucin binding strength confirms mucoadhesive nature of Br‐EuNCs. Pharmacokinetic study in rats indicates 2.89‐fold increase in bromelain bioavailability after its encapsulation. Subsequently, pharmacodynamic studies reveal that optimized formulation significantly (p <0.05) reduces spasm, bronchial hyper‐responsiveness and inhibits convulsions in the ovalbumin induced asthma model. In addition, Br‐EuNCs significantly regulate hematological, biochemical, and immunological parameters disturbed during ovalbumin exposure. Histological studies of lung show significant protectionoffered by formulation against tissue damage during asthma. The results suggest that therapeutic performance of bromelain can be improved utilizing Br‐EuNCs as a platform via nasal route for asthma management.

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Targeted delivery of bromelain using dual mode nanoparticles: synthesis, physicochemical characterization, in vitro and in vivo evaluation

Abstract: The engineering, characterization, and application of dual-functional delivery vehicle “SPIONs–Br–FA” are reported.

Cited by 27 publications

References 97 publications

Bromelain inhibitory effect on colony formation: An In vitro Study on human AGS, PC3, and MCF7 cancer cells

Bromelain inhibitory effect on colony formation: An In vitro Study on human AGS, PC3, and MCF7 cancer cells

Rationally designed curcumin laden glycopolymeric nanoparticles: Implications on cellular uptake and anticancer efficacy

Mucoadhesive Cationic Bromelain Laden Nanocarriers Restore Patency of Airway Hyperresponsive Remodeling via Nasal Route

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