Ceruloplasmin Concentration in Human Colostrum and Mature Milk

Kiyosawa, Isao; Matsuyama, Jun; Nyui, Shoko; Fukuda, Ai

doi:10.1271/bbb.59.713

Cited by 20 publications

(12 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The complex is the only breast milk component with ferroxidase activity, i.e., the whole bulk of Cp in milk interacts with Lf. It is known that Cp concentration in milk is low, being 0.3 lM in colostrum and 40 nM in mature milk (Kiyosawa et al 1995), which contradicts the K d value *1.8 lM obtained previously (Zakharova et al 2000). The presence of the Cp-Lf complex in plasma where Lf content remains low even after a disease-caused increase also calls in question the K d value.…”

Section: Introductionmentioning

confidence: 75%

Effect of lactoferrin on oxidative features of ceruloplasmin

et al. 2009

View full text Add to dashboard Cite

In our previous report we first described a complex between lactoferrin (Lf) and ceruloplasmin (Cp) with K (d) approximately 1.8 microM. The presence of this complex in colostrum that never contains more than 0.3 microM Cp questions the reliability of K (d) value. We carefully studied Lf binding to Cp and investigated the enzymatic activity of the latter in the presence of Lf, which allowed obtaining a new value for K (d) of Cp-Lf complex. Lf interacting with Cp changes its oxidizing activity with various substrates, such as Fe(2+), o-dianisidine (o-DA), p-phenylenediamine (p-PD) and dihydroxyphenylalanine (DOPA). The presence of at least two binding sites for Lf in Cp molecule is deduced from comparison of substrates' oxidation kinetics with and without Lf. When Lf binds to the first site affinity of Cp to Fe(2+) and to o-DA increases, but it decreases towards DOPA and remains unchanged towards p-PD. Oxidation rate of Fe(2+) grows, while that of o-DA, p-PD and DOPA goes down. Subsequent Lf binding to the second center has no effect on iron oxidation, hampers DOPA and o-DA oxidation, and reduces affinity towards p-PD. Scatchard plot for Lf sorbing to Cp-Sepharose allowed estimating K (d) for Lf binding to high-affinity (approximately 13.4 nM) and low-affinity (approximately 211 nM) sites. The observed effect of Lf on ferroxidase activity of Cp is likely to have physiological implications.

show abstract

Section: Introductionmentioning

confidence: 75%

Effect of lactoferrin on oxidative features of ceruloplasmin

et al. 2009

View full text Add to dashboard Cite

show abstract

“…The CP concentrations in human colostrum and mature milk at <1 month after parturition were 4.45 and 4.09 mg per 100 ml, respectively. In mature milk after >1 month, the CP concentration had decreased to 1.7 mg per 100 ml (Kiyosawa et al. 1995).…”

Section: Othersmentioning

confidence: 99%

Antioxidants in Bovine Colostrum

Przybylska¹,

Albera²,

Kankofer³

2007

Reprod Domestic Animals

View full text Add to dashboard Cite

Quality, content and properties of colostrum are crucial for the neonate and its further development. Due to essential differences between intrauterine and extrauterine environment, the neonate is exposed to oxidative stress conditions. Colostrum apart from nutrient and immunological components should contain antioxidative systems necessary for the protection against reactive oxygen species. This review describes available data on enzymatic and non-enzymatic antioxidants in colostrum. Due to the fact that the literature concerning bovine colostrum is scanty, the information based on bovine mature milk determinations as well as other species is provided. Bovine colostrum is used not only by calves, but also for the production of hyperimmunized colostrum, medicines or feed supplements. Quality of colostrum influences quality of mature milk. This is another reason, except from health of neonate, why antioxidative properties of bovine colostrum are of special importance and require further detailed elucidation.

show abstract

“…Our previous studies (Cerveza et al 2000;Donley et al 2002) and those of some others (Kiyosawa et al 1995) had indicated that up to about 25% of the copper in the milk of rats and humans was due to ceruloplasmin produced by the mammary gland (Cerveza et al 2000;Donley et al 2002). In mice, the decrease in milk copper was about twice the expected 25%.…”

Section: Discussionmentioning

confidence: 80%

“…In the mammary gland, too, it is likely that ATP7B is necessary for copper incorporation into ceruloplasmin (in the trans Golgi network), and for importing copper into the milk, since mutations that impair the activity of this copper pump (such as those of the toxic milk [tx] mouse) reduce milk copper concentrations about 6-fold (Michalczyk et al 2000;Rauch 1983). We and some others have determined by immunoassays, copper radiolabeling and ceruloplasmin enzyme activity assays that up to about 25% of the copper in the post-colostrum milk of rats and humans is associated with ceruloplasmin (Cerveza et al 2000;Donley et al 2002;Kiyosawa et al 1995). Platonova et al (2007) contend that all of human milk copper is with ceruloplasmin, and that it is absorbed as the whole protein by newborn rats.…”

Section: Introductionmentioning

confidence: 99%

Lack of ceruloplasmin expression alters aspects of copper transport to the fetus and newborn, as determined in mice

et al. 2011

View full text Add to dashboard Cite

Copper transport and accumulation were studied in virgin and lactating C57BL/6 mice, with and without expression of ceruloplasmin (Cp), to assess the importance of Cp to these processes. One hour after i.p. injection of tracer (64)Cu, liver and kidney accounted for 80% of the radioactivity, and mammary gland 1%, while in lactating Cp+/+ mice 2-4 days post partum, uptake by mammary gland was 9-fold higher and that of liver and other organs was decreased, with (64)Cu rapidly appearing in milk. Parallel studies in Cp-/- mice (siblings from same colony) gave virtually identical results. However, their milk contained less (64)Cu, and actual copper contents determined by furnace atomic absorption were less than half those for milk from normal dams. Liver copper concentrations of pups born to Cp-/- dams also were half those of pups from wild type dams. Copper in pup brains was unaffected; but iron concentrations were reduced. We conclude that absence of Cp, while not affecting entry of exchangeable copper from the blood into the mammary gland, does have a significant effect on the availability of this metal to the newborn through the milk and in the form of stores accumulating in gestation.

show abstract

Ceruloplasmin Concentration in Human Colostrum and Mature Milk

Cited by 20 publications

References 4 publications

Effect of lactoferrin on oxidative features of ceruloplasmin

Effect of lactoferrin on oxidative features of ceruloplasmin

Antioxidants in Bovine Colostrum

Lack of ceruloplasmin expression alters aspects of copper transport to the fetus and newborn, as determined in mice

Contact Info

Product

Resources

About