2019
DOI: 10.1016/j.vascn.2019.01.002
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Cerulein-induced chronic pancreatitis in Swiss albino mice: An improved short-term model for pharmacological screening

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Cited by 15 publications
(18 citation statements)
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“…Malondialdehyde (MDA), nitrite, and GSH contents were measured in pancreatic tissue by following the previously described method 27,28 . Pancreatic tissue samples were homogenized in phosphate buffer and homogenates were used for the estimation of MDA levels and remaining tissue homogenate were centrifuged at 9168 g for 10 minutes at 4°C and the supernatant was used for the estimation of nitrite and GSH content in the pancreas.…”
Section: Methodsmentioning
confidence: 99%
“…Malondialdehyde (MDA), nitrite, and GSH contents were measured in pancreatic tissue by following the previously described method 27,28 . Pancreatic tissue samples were homogenized in phosphate buffer and homogenates were used for the estimation of MDA levels and remaining tissue homogenate were centrifuged at 9168 g for 10 minutes at 4°C and the supernatant was used for the estimation of nitrite and GSH content in the pancreas.…”
Section: Methodsmentioning
confidence: 99%
“…The expression of proteins was evaluated by western blot analysis. The samples for western blot were prepared as described earlier . A total of 20 μg of protein was loaded onto polyacrylamide gel, and the proteins were separated by SDS‐PAGE (sodium dodecyl sulfate‐polyacrylamide gel electrophoresis) and transferred onto the nitrocellulose membranes at 17 V for 55 min.…”
Section: Methodsmentioning
confidence: 99%
“…The samples for western blot were prepared as described earlier. 24,25 A total of 20 μg of protein was loaded onto polyacrylamide gel, and the proteins were separated by SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) and transferred onto the nitrocellulose membranes at 17 V for 55 min. The membranes were blocked with 3% BSA and washed with TBS-T for 10 min thrice and subsequently incubated overnight with the respective primary antibodies (1:1000 dilution) at 4 C. Next day, the membranes were washed with TBS-T and incubated with secondary antibody (1:20,000 dilution) at room temperature for 1 hr.…”
Section: Western Blot Analysismentioning
confidence: 99%
“…Doses of IMT were selected on the basis of previous literature [20]. However, cerulein needed minimum 12 hr to produce maximum in ammatory response, IMT was given every other day after cerulein exposures for the period of 3 weeks [19].…”
Section: Animals and Experimental Designmentioning
confidence: 99%