Cerium oxide nanoparticles stimulate proliferation of primary mouse embryonic fibroblasts in vitro

Попов, А. И.; Popova, N.R.; Селезнева, И. И.; Akkizov, Azamat Y.; Ivanov, V. K.

doi:10.1016/j.msec.2016.05.103

Cited by 73 publications

(54 citation statements)

References 53 publications

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“…CNPs have been shown to enhance primary mouse embryonic fibroblast proliferation at similar low dose range, which was attributed to its ability to maintain redox balance for enhanced intracellular homeostasis and metabolism. 53 Our results indicate that shape-specific CNPs do not exhibit acute cytotoxicity in the dose range of 0.01– 100 μ g/mL.…”

Section: Resultsmentioning

confidence: 62%

Shape-Specific Nanoceria Mitigate Oxidative Stress-Induced Calcification in Primary Human Valvular Interstitial Cell Culture

et al. 2017

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Introduction Lack of effective pharmacological treatment makes valvular calcification a significant clinical problem in patients with valvular disease and bioprosthetic/mechanical valve replacement therapies. Elevated levels of reactive oxygen species (ROS) in valve tissue have been identified as a prominent hallmark and driving factor for valvular calcification. However, the therapeutic value of ROS-modulating agents for valvular calcification remains elusive. We hypothesized that ROS-modulating shape-specific cerium oxide nanoparticles (CNPs) will inhibit oxidative stress-induced valvular calcification. CNPs are a class of self-regenerative ROS-modulating agents, which can switch between Ce3+ and Ce4+ in response to oxidative microen-vironment. In this work, we developed oxidative stress-induced valve calcification model using two patient-derived stenotic valve interstitial cells (hVICs) and investigated the therapeutic effect of shape-specific CNPs to inhibit hVIC calcification. Methods Human valvular interstitial cells (hVICs) were obtained from a normal healthy donor and two patients with calcified aortic valves. hVICs were characterized for their phenotypic (mesenchymal, myofibroblast and osteoblast) marker expression by qRT-PCR and antioxidant enzymes activity before and after exposure to hydrogen peroxide (H2O2)-induced oxidative stress. Four shape-specific CNPs (sphere, short rod, long rod, and cube) were synthesized via hydrothermal or ultra-sonication method and characterized for their biocompatibility in hVICs by alamarBlue® assay, and ROS scavenging ability by DCFH-DA assay. H2O2 and inorganic phosphate (Pi) were co-administrated to induce hVIC calcification in vitro as demonstrated by Alizarin Red S staining and calcium quantification. The effect of CNPs on inhibiting H2O2-induced hVIC calcification was evaluated. Results hVICs isolated from calcified valves exhibited elevated osteoblast marker expression and decreased antioxidant enzyme activities compared to the normal hVICs. Due to the impaired antioxidant enzyme activities, acute H2O2-induced oxidative stress resulted in higher ROS levels and osteoblast marker expression in both diseased hVICs when compared to the normal hVICs. Shape-specific CNPs exhibited shape-dependent abiotic ROS scavenging ability, and excellent cytocompatibility. Rod and sphere CNPs scavenged H2O2-induced oxidative stress in hVICs in a shape- and dose-dependent manner by lowering intracellular ROS levels and osteoblast marker expression. Further, CNPs also enhanced activity of antioxidant enzymes in hVICs to combat oxidative stress. Cube CNPs were not effective ROS scavengers. The addition of H2O2 in the Pi-induced calcification model further increased calcium deposition in vitro in a time-dependent manner. Co-administration of rod CNPs with Pi and H2O2 mitigated calcification in the diseased hVICs. Conclusions We demonstrated that hVICs derived from calcified valves exhibited impaired antioxidant defense mechanisms and were more susceptible to oxidative stress th...

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Section: Resultsmentioning

confidence: 62%

Shape-Specific Nanoceria Mitigate Oxidative Stress-Induced Calcification in Primary Human Valvular Interstitial Cell Culture

et al. 2017

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“…Previously it was shown that they can be used for encapsulating proteins [20], DNA [31], RNA [32], pharmaceutical formulations [33] and other compounds. A layer-by-layer method of synthesizing polyelectrolyte microcapsules is based on the use of differently charged polyelectrolytes that are alternately adsorbed on an organic (polymeric) or inorganic (oxides, calcium carbonate) substrate [34].…”

Section: The Hypothesis Assessment and Reviewmentioning

confidence: 99%

“…Cerium oxide nanoparticles have SOD-mimetic activity and inactivate superoxide radicals [17], and their ability to inactivate hydrogen peroxide is comparable to that of catalase [18,19]. Using a variety of surface modifiers and synthetic methods allows one to vary the size, shape, and charge of cerium oxide nanoparticles that affect their physical and chemical characteristics, including the level of antioxidant activity, and consequently intracellular biological effects [16,[20][21][22]. The presence of oxygen vacancies (defects in a crystal lattice) and auto-regenerative oxidation-reduction cycle (Ce 3+ ←→ Ce 4+ ) enable the use of cerium oxide nanoparticles as broad-spectrum antioxidant drugs at the neutral pH found in healthy tissues [23,24].…”

Section: Introductionmentioning

confidence: 99%

Layer-by-layer capsules as smart delivery systems of CeO2 nanoparticle-based theranostic agents

Popova¹,

Попов²,

Shcherbakov³

et al. 2017

Nanosystems: Phys. Chem. Math.

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Modern methods of cancer treatment include chemotherapy and radiotherapy, but they are often characterized by low efficacy and high toxicity. The effectiveness of cancer therapy is often limited by a lack of effective systems for drug delivery to the tumor site. Cerium oxide nanoparticles are able to act as radioprotectors and as radiosensitizers exhibiting selective toxicity in the tumor microenvironment, providing for their tremendous potential in treating cancer. However, methods for controlled delivery of CeO 2 nanoparticles to the tumor have not been investigated nor described yet. In this article, we consider different approaches to the development of new ceria nanoparticle-based theranostic agents. Modification of polyelectrolyte microcapsules with nano-ceria appears to be the most promising method. Our design proposals are based on the synergistic pharmacological action of ceria-based nanomaterials and anticancer pharmaceuticals with the ability to control and visualize their sites of localization.

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“…They postulated that cerium oxide nanoparticles stimulated the proliferation of a cell in a wide range of concentrations, ranging from 10 −3 М to 10−9 M, through the reduction of intracellular levels of ROS at the lag phase of the cell growth. Finally, they concluded that nanocrystalline ceria could be considered as a basis for the effective and affordable source of supplements during cell culturing [74].…”

Section: Recent Application In Pharmacological Studiesmentioning

confidence: 99%

Between the Bioactive Extracts of Edible Mushrooms and Pharmacologically Important Nanoparticles: Need for the Investigation of a Synergistic Combination - A Mini Review

Ukaegbu

Jalal²,

Rashid³

et al. 2017

Asian J Pharm Clin Res

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The pharmacological potential of bioactive compounds extracted from mushrooms has been studied to a reasonable level. In the same vein, the bioactivity of nanoparticles has also been investigated and reported to be of potential pharmacological benefit. No doubt, there is a reasonable amount of claims regarding the vast activity of the mushroom extracts and nanoparticles on the tested cell lines and microorganisms. In this paper, a review of the recent application of bioactive compounds extracted from two edible mushrooms (Coprinus comatus and Lactarius deliciosus), as well as some of the recently reported studies on some nanoparticles of pharmacological potentials, was carried out. In order to check for synergy in the bioactivity of the mushroom extracts when co-administered with nanoparticles, an investigation on the synergistic application of the materials through the encapsulation of the bioactive extracts from the mushroom onto the nanoparticle was proposed. The supposed synergy in the activity of the extract-nanoparticle complex could hold the key to improved activity of nutraceuticals against resistant microorganisms and tumor cells.

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Cerium oxide nanoparticles stimulate proliferation of primary mouse embryonic fibroblasts in vitro

Cited by 73 publications

References 53 publications

Shape-Specific Nanoceria Mitigate Oxidative Stress-Induced Calcification in Primary Human Valvular Interstitial Cell Culture

Shape-Specific Nanoceria Mitigate Oxidative Stress-Induced Calcification in Primary Human Valvular Interstitial Cell Culture

Layer-by-layer capsules as smart delivery systems of CeO2 nanoparticle-based theranostic agents

Between the Bioactive Extracts of Edible Mushrooms and Pharmacologically Important Nanoparticles: Need for the Investigation of a Synergistic Combination - A Mini Review

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