Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue

Zhang, Xueli; Kuo, Chaincy; Moore, Anna; Ran, Chongzhao

doi:10.3791/51790

Cited by 9 publications

(8 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Perkin Elmer micro-CT was applied to analyze brown adipose tissue as described in previous studies ( 40 , 41 ). Briefly, mice were anesthetized with 30 mg/kg pentobarbital sodium and then scanned in the machine to measure BAT volume in the scapula.…”

Section: Methodsmentioning

confidence: 99%

Brown Adipose Tissue Rescues Bone Loss Induced by Cold Exposure

et al. 2022

Front. Endocrinol.

View full text Add to dashboard Cite

Cold temperature activates the sympathetic nervous system (SNS) to induce bone loss by altering bone remodeling. Brown adipose tissue (BAT) is influenced by the SNS in cold environments. Many studies have confirmed a positive relationship between BAT volume and bone mass, but the influence and mechanism of BAT on bone in vivo and in vitro is still unknown. Two-month-old C57/BL6j male mice were exposed to cold temperature (4°C) to induce BAT generation. BAT volume, bone remodeling and microstructure were assessed after 1 day, 14 days and 28 days of cold exposure. CTX-1, P1NP and IL-6 levels were detected in the serum by ELISA. To determine the effect of BAT on osteoclasts and osteoblasts in vitro, brown adipocyte conditional medium (BAT CM) was collected and added to the differentiation medium of bone marrow-derived macrophages (BMMs) and bone marrow mesenchymal stem cells (BMSCs). Micro-CT results showed that the bone volume fraction (BV/TV, %) significantly decreased after 14 days of exposure to cold temperature but recovered after 28 days. Double labeling and TRAP staining in vivo showed that bone remodeling was altered during cold exposure. BAT volume enlarged after 14 days of cold stimulation, and IL-6 increased. BAT CM promoted BMSC mineralization by increasing osteocalcin (Ocn), RUNX family transcription factor 2 (Runx2) and alkaline phosphatase (Alp) expression, while bone absorption was inhibited by BAT CM. In conclusion, restoration of bone volume after cold exposure may be attributed to enlarged BAT. BAT has a beneficial effect on bone mass by facilitating osteogenesis and suppressing osteoclastogenesis.

show abstract

Section: Methodsmentioning

confidence: 99%

Brown Adipose Tissue Rescues Bone Loss Induced by Cold Exposure

et al. 2022

Front. Endocrinol.

View full text Add to dashboard Cite

show abstract

“…In addition, other optical approaches, including Cerenkov luminescence imaging, bioluminescence imaging, and photoacoustic imaging, were reported for BAT imaging. Our group has reported that Cerenkov luminescence imaging with 18 F-FDG could be used to monitor BAT activation under different conditions [104,105]. Cheng et al reported that CyHF-8 was capable of noninvasively detecting interscapular BAT with both NIRF and photoacoustic imaging [106].…”

Section: Near-infrared Fluorescence Imaging (Nirfi)mentioning

confidence: 99%

Molecular Imaging of Brown Adipose Tissue Mass

Yang

Zhang

Parhat

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

Brown adipose tissue (BAT), a uniquely thermogenic tissue that plays an important role in metabolism and energy expenditure, has recently become a revived target in the fight against metabolic diseases, such as obesity, diabetes, and non-alcoholic fatty liver disease (NAFLD). Different from white adipose tissue (WAT), the brown adipocytes have distinctive features including multilocular lipid droplets, a large number of mitochondria, and a high expression of uncoupling protein-1 (UCP-1), as well as abundant capillarity. These histologic characteristics provide an opportunity to differentiate BAT from WAT using imaging modalities, such as PET/CT, SPECT/CT, MRI, NIRF and Ultrasound. However, most of the reported imaging methods were BAT activation dependent, and the imaging signals could be affected by many factors, including environmental temperatures and the states of the sympathetic nervous system. Accurate BAT mass detection methods that are independent of temperature and hormone levels have the capacity to track the development and changes of BAT throughout the lifetime of mammals, and such methods could be very useful for the investigation of potential BAT-related therapies. In this review, we focus on molecular imaging modalities that can detect and quantify BAT mass. In addition, their detection mechanism and limitations will be discussed as well.

show abstract

“…Optical techniques are an emerging tool for BAT imaging, especially in rodents. It has been shown that Cerenkov luminescence imaging (CLI) with 18 F-FDG can be used to optically image BAT in small animals ( 25 ). Even though cheaper and faster than PET, CLI again comes with the need for radiotracer injection.…”

Section: Introductionmentioning

confidence: 99%

Magnetic Resonance Imaging Techniques for Brown Adipose Tissue Detection

Junker

Branca

et al. 2020

Front. Endocrinol.

View full text Add to dashboard Cite

Magnetic resonance imaging (MRI) and magnetic resonance spectroscopy (MRS) methods can non-invasively assess brown adipose tissue (BAT) structure and function. Recently, MRI and MRS have been proposed as a means to differentiate BAT from white adipose tissue (WAT) and to extract morphological and functional information on BAT inaccessible by other means. Specifically, proton MR (1 H) techniques, such as proton density fat fraction mapping, diffusion imaging, and intermolecular multiple quantum coherence imaging, have been employed to access BAT microstructure; MR thermometry, relaxometry, and MRI and MRS with 31 P, 2 H, 13 C, and 129 Xe have shown to provide complementary information on BAT function. The purpose of the present review is to provide a comprehensive overview of MR imaging and spectroscopy techniques used to detect BAT in rodents and in humans. The present work discusses common challenges of current methods and provides an outlook on possible future directions of using MRI and MRS in BAT studies.

show abstract

Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue

Cited by 9 publications

References 43 publications

Brown Adipose Tissue Rescues Bone Loss Induced by Cold Exposure

Brown Adipose Tissue Rescues Bone Loss Induced by Cold Exposure

Molecular Imaging of Brown Adipose Tissue Mass

Magnetic Resonance Imaging Techniques for Brown Adipose Tissue Detection

Contact Info

Product

Resources

About