CEREBROSPINAL-FLUID IgM IN THE ABSENCE OF SERUM-IgM IN COMBINED IMMUNODEFICIENCY

Lord, RalphA.; Goldblum, RandallM.; Forman, PhillipM.; Dupree, Elton; Storey, WrayD.; Goldman, Armond S.

doi:10.1016/s0140-6736(73)92349-0

Cited by 19 publications

(4 citation statements)

References 9 publications

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“…Certain pathologic conditions of the central nervous system are associated with increased levels of immunoglobulins in the CSF (1,9,17,22), some of which are locally produced (1,17,20). Data from the present study (Table 3, Table 3 refer to anticysticercus antibodies.…”

Section: Discussionmentioning

confidence: 57%

Characterization by enzyme-linked immunosorbent assay of the humoral immune response in patients with neurocysticercosis and its application in immunodiagnosis

Espinoza¹,

Ruiz-Palacios²,

Tovar³

et al. 1986

J Clin Microbiol

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The enzyme-linked immunosorbent assay was standardized for the search for specific antibodies in human neurocysticercosis. A crude cysticercal extract and two partially purified antigenic fractions were used, as well as serum and cerebrospinal fluid (CSF) samples of different groups of subjects. Immunoglobulin G (IgG) antibodies were detected in serum and CSF, with a sensitivity of 85 and 90%, respectively. Specificity was 96% with a partially purified antigen and 100% with the crude cysticercal extract. IgM and IgA antibodies were detected less frequently, and IgE was detected only occasionally, both in serum and CSF. Analysis of serum and CSF samples drawn from the same patient did not always reveal the presence of anticysticercus antibodies in both samples. A significant correlation was found between the presence or absence of IgG antibodies in the CSF and the morphological appearance of the parasite (undamaged or calcified). Variations in the humoral response were not found to correlate with clinical and laboratory findings.

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Section: Discussionmentioning

confidence: 57%

Characterization by enzyme-linked immunosorbent assay of the humoral immune response in patients with neurocysticercosis and its application in immunodiagnosis

Espinoza¹,

Ruiz-Palacios²,

Tovar³

et al. 1986

J Clin Microbiol

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“…It obviously ap pears in cerebrospinal fluid (C SF ) with impor tant elevation o f total protein (5), especially in bacterial (4 -1 2 ) and viral ( 2 -1 1) meningitis. A local (intrathecal) synthesis o f IgM was sug gested by Savory and Heintges (7) and demon strated by Lord et al (3) in a case with absence of serum IgM. Sandberg-Wollheim (6) recently demonstrated an in vitro IgM synthesis from C SF lymphocytes in meningo-encephalitis of presumed viral origin.…”

Section: Sclerosismentioning

confidence: 88%

Serum and Cerebrospinal Fluid IGM in 203 Neurological Patients

Schuller¹,

Delasnerie²,

Helary³

et al. 1978

Eur Neurol

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Simultaneous serum and CSF IgM determinations were made by electroimmunodiffusion in 5 normals and 203 patients. Absent from normal CSF, IgM was found in 56 patients (27%): (1) A blood-CSF transudation was present in 25 patients (12%). IgM seems of no diagnostic value in these cases. (2) A purely local synthesis was demonstrated in 17 patients (8%), concerning proved (bacterial, viral, parasitic) or supposed (multiple sclerosis) persistent infectious aggressions. (3) In the last 14 patients with IgM in CSF, a transudation was associated with a local synthesis of IgG (meningitic pattern). (4) A ratio IgM 10³ /albumin above 10 seems an argument for multiple sclerosis.

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“…At age 1-2 years serum IgG, IgM, and IgA are 66, 59, and 25% of respective adult values (Stiehm and Fudenberg, 1966). It is believed that CSF immunoglobulins are derived by diffusion from serum, although the lymphoid tissue may produce IgG (Lord et al, 1973).…”

Section: Discussionmentioning

confidence: 99%

Cerebrospinal fluid immunoglobulins in children.

Gill¹,

Brody²

1979

Archives of Disease in Childhood

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SUMMARY Cerebrospinal fluid (CSF) immunoglobulins were measured in 62 normal children, in 9 children with purulent meningitis, and in 10 children with presumptive viral meningitis. The mean values in normal children were IgA 0, IgM 0, and IgG 0 84 ± 1 4 mg/100 ml (± SD). The mean levels of all CSF immunoglobulins were raised in acute bacterial meningitis and were significantly greater than the levels found in viral meningitis. CSF IgM was 0 16 ± 0 5 mg/100 ml in viral meningitis compared with 2 64 + 2 06 mg/100 ml in bacterial meningitis (P<0 01). However, these values overlapped to a considerable extent and, generally, measurement of CSF immunoglobulins did not enhance diagnostic accuracy in this group of children. Smith et al. (1973) presented data on CSF immunoglobulins in meningitis in adults, and suggested that the levels of IgM might help to distinguish viral from bacterial meningitis. Methods and materialCSF immunoglobulins were measured in 81 children aged between 0-1 and IOj years. The indications for spinal tap were meningitis, suspected meningitis, convulsions, or (for therapeutic purposes) leukaemia. The 62 children designated as normal had WBC <10 x 106/1 (<5 x 106/1 in all except 4), and normal CSF protein and glucose relative to age. Most of the children were being evaluated for suspected meningitis or because they had had febrile convulsions. Nine children were considered to have bacterial meningitis, of which 3 proved to be due to Haemophilus influenzae, 3 to Neisseria meningitidis, 1 to Staphylococcus albus, and 2 showed no growth on culture. In 10 children meningitis was presumed to be viral in origin on the basis of leucocyte response, CSF protein and glucose levels, and clinical course. Specific viral cultures were not performed.Lumbar puncture was performed as indicated on admission and the CSF analysed routinely in the laboratory. An unspun aliquot of the CSF was stored at 40C before the immunoglobulins were estimated. CSF immunoglobulins were measured without knowledge of the child's clinical or routine CSF findings. Cerebrospinal fluid samples which were grossly blood-stained or which contained RBC >100 x 106/1 were discarded.CSF immunoglobulins concentrations were measured by the radial immunodiffusion method (Mancini et al., 1965), using commercial L.C. Partigen plates and pooled human serum (Behring) as standard. 5 V.1 centrifuged, undiluted CSF was placed in the application wells and incubated for 72 hours at room temperature. Appropriate dilutions of standard in normal saline were also prepared. To intensify the precipitin ring the IgM and IgA plates were rinsed in phosphate-buffered saline for 24 hours, then covered with a 4% aqueous solution of tannin for 30 minutes and washed with distilled water. Standard curves were prepared from the square of the precipitin rings.Results Table 1 shows that IgA and IgM were not detected in Table 1 17-88 + 6-21 Conversion: traditional units to SI-CSF proteins: 1 mg/100 ml ;w 0.01 g/l, glucose: 1 mg/100 ml m 0-0555 mmol/l.

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CEREBROSPINAL-FLUID IgM IN THE ABSENCE OF SERUM-IgM IN COMBINED IMMUNODEFICIENCY

Cited by 19 publications

References 9 publications

Characterization by enzyme-linked immunosorbent assay of the humoral immune response in patients with neurocysticercosis and its application in immunodiagnosis

Characterization by enzyme-linked immunosorbent assay of the humoral immune response in patients with neurocysticercosis and its application in immunodiagnosis

Serum and Cerebrospinal Fluid IGM in 203 Neurological Patients

Cerebrospinal fluid immunoglobulins in children.

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