Characterization by enzyme-linked immunosorbent assay of the humoral immune response in patients with neurocysticercosis and its application in immunodiagnosis

Espinoza, Bertha; Ruiz-Palacios, G M; Tovar, Armando R.; Sandoval, Miguel A.; Plancarte, A.; Flisser, Ana

doi:10.1128/jcm.24.4.536-541.1986

Cited by 71 publications

(28 citation statements)

References 12 publications

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“…Several studies have shown that immunoenzymatic tests for neurocysticercosis are less likely to react in patients with a single cyst or calcified parasites than in those with multiple non‐calcified parasites (Espinoza et al. 1986; Pammenter & Rossouw 1987; Baily et al.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Taenia solium and Taenia crassiceps cysticercal antigens for the serodiagnosis of neurocysticercosis

Arruda

Silva

Quagliato

et al. 2005

Tropical Med Int Health

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SummaryWe evaluated the usefulness of seven cysticercal antigen extracts, four from Taenia solium cysticerci (whole parasite-TsoW, membrane-TsoMe, vesicular fluid-TsoVF and scolex-TsoSc) and three from T. crassiceps cysticerci (whole parasite-TcraW, membrane-TcraMe and vesicular fluid-TcraVF), for serodiagnosis of neurocysticercosis with an enzyme-linked immunosorbent assay (ELISA). Cysticercusspecific IgG were screened in serum samples from 23 patients with neurocysticercosis, 32 patients with other infections and 48 healthy persons. The best results were obtained with the TsoVF-ELISA (91.3% sensitivity; 96.2% specificity) and TcraVF-ELISA (91.3% sensitivity; 95% specificity). The ELISA done with whole parasite and membrane extracts from cysts of T. solium and T. crassiceps and the scolex extract from T. solium cysts showed a low performance in terms of sensitivity, ranging from 47.8% to 73.9%. None of the antigen preparations from T. solium and T. crassiceps cysticerci used in this study showed outstanding performance for the serodiagnosis of neurocysticercosis. However, considering the results obtained with the seven antigen preparations, vesicular fluid from T. solium and T. crassiceps cysticerci may be useful for detecting specific antibodies in sera from patients with neurocysticercosis.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Taenia solium and Taenia crassiceps cysticercal antigens for the serodiagnosis of neurocysticercosis

Arruda

Silva

Quagliato

et al. 2005

Tropical Med Int Health

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“…A great heterogeneity regarding the clinical features of the patients is evident from the present study in accordance with many other reports (Correa et al, 1985;Correa er al., 1986;Zenteno et al, 1982;Flisser, 1988). Nonetheless, all patients suffered from endocranial hypertension in spite of the parasite location and 53.3% had a chronic disease.…”

Section: Discussionmentioning

confidence: 98%

“…and B, a secretion antigen (Guerra et al, 1982) that is recognized by approx. 90% of patients (Espinoza et al, 1986). Cell proliferation was measured by incorporation of tritiated thymidine, added 18 h before harvesting.…”

Section: Evaluation Of Host Immune Responsementioning

confidence: 99%

“…Trejo et al subarachnoid space, ventricular system or spinal cord (Del Brutto er al., 1988). Neurocysticercosis is a complex disease and thus, the clinical manifestations are extremely varied and nonspecific, depending on factors such as number of parasites, location, extent of inflammatory reaction and immune status or general conditions of the host (Flisser, 1988). Therefore, the establishment of an 'in equilibrium host-parasite relationship' determines the survival of both host and infectious agent.…”

Section: Introductionmentioning

confidence: 99%

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What Is the Significance of the Presence of MHC Molecules on the Surface of Parasites in Human Neurocysticercosis?

Trejo

Talamas

Granados

et al. 1989

Int J Immunogenetics

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SUMMARY Sixteen cysticerci excised from 15 surgery patients were examined for the presence of HLA molecules on their surface, to confirm the role of these molecules in parasite damage and to investigate if HLA products are host specific or perhaps host‐like antigens synthesized by the parasite. MoAbs against monomorphic and polymorphic HLA were chosen according to the patients HLA phenotypes. MoAbs against host and non‐host antigens were selected and tested on cyst slides by indirect immunofluorescence assays. Host molecules were present in 43.7% of the cysts, but non‐host antigens were also apparent in 62.5%. These results suggest mimicry as a possible mechanism to explain the presence of MHC products on the surface of the parasite; inflammation may also induce the expression of HLA that could become associated with the parasite. In vitro cellular immune response to specific antigens was also performed and positive responses correlated with the presence of HLA molecules on the cyst's surface. Moreover, damaged parasites had host molecules as well. Parasites from responder patients had all kind of HLA molecules or at least, antigenic determinants while the cysts from non‐responders did not have molecules on their surface. These data support the role of HLA in cyst destruction.

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“…Sensitivity of IgG antibody detection was 85% with CE and 80% with AgB. Specificity was 100% with CE and 96% with AgB (16).…”

Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 90%

Contribution of immunodiagnostic tests to epidemiological/intervention studies of cysticercosis/taeniosis in México

Flisser

Gyorkos

2007

Parasite Immunology

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Cysticercosis is acquired when swine ingest human faeces contaminated with Taenia solium eggs. Humans become tapeworm carriers when they ingest infected pork meat. They can also develop cysticercosis after inadvertently swallowing T. solium eggs. Human neurocysticercosis (NCC) is considered as a public health problem in Mexico and in several countries around the world, mainly developing ones. The development of immunodiagnostic techniques has promoted the conduct of seroepidemiological studies. This review provides insight into the evolution of these techniques, their predictive values and their use in field studies, and summarizes evidence supporting health care practice and policy related to cysticercosis/taeniosis in Mexico. Serological studies in rural and urban settings have demonstrated that close proximity with a tapeworm carrier is the main risk factor for acquiring cysticercosis. Research focusing on the tapeworm carrier generated an ELISA for the detection of Taenia coproantigens and facilitated the evaluation of intervention measures. Health education and self-identification of tapeworm carriers were shown to be successful. However, cestodial treatment as a community-based intervention was not as successful. Current immunodiagnostic techniques can be used to pinpoint transmission foci so that appropriate and effective interventions can be applied. In this way, sustainable control, and even eradication of T. solium may be envisioned.

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Characterization by enzyme-linked immunosorbent assay of the humoral immune response in patients with neurocysticercosis and its application in immunodiagnosis

Cited by 71 publications

References 12 publications

Evaluation of Taenia solium and Taenia crassiceps cysticercal antigens for the serodiagnosis of neurocysticercosis

Evaluation of Taenia solium and Taenia crassiceps cysticercal antigens for the serodiagnosis of neurocysticercosis

What Is the Significance of the Presence of MHC Molecules on the Surface of Parasites in Human Neurocysticercosis?

Contribution of immunodiagnostic tests to epidemiological/intervention studies of cysticercosis/taeniosis in México

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