Abstract:Multiple sclerosis (MS)-related inflammation can be divided into lesional activity, mediated by immune cells migrating from the periphery to the central nervous system (CNS) and non-lesional activity, mediated by inflammation compartmentalized to CNS tissue. Lesional inflammatory activity, reflected by contrast-enhancing lesions (CELs) on the magnetic resonance imaging (MRI), is effectively inhibited by current disease modifying therapies (DMTs). While, the effect of DMTs on non-lesional inflammatory activity … Show more
“…As serum/plasma NfL levels increase with physiological aging (Disanto et al, 2017), the measured NfL concentrations were adjusted for effect of healthy aging as described previously (Masvekar R et al, 2021). Following age vs serum-or plasma-NfL equations from HC cohorts were used: ln(serum NfL) = 0.0177*Age + 0.9696 and ln(plasma NfL) = 0.0158*Age + 1.247.…”
Section: Adjustment For Effect Of Healthy Agingmentioning
confidence: 99%
“…Serum and plasma samples from healthy controls (HC) and multiple sclerosis (MS) subjects were collected at Neuroimmunological Diseases Section (NDS), NIAID after informed consent under IRB-approved protocol (ClinicalTrials.gov: NCT00794352). The NfL levels measured in HC and MS subgroups were previously reported (Masvekar R et al, 2021) and are used in the current study only as a positive control of neuronal injury; the measurements of other COVID-19 prognostic biomarkers in these control samples were not reported previously. 378 serum or plasma samples were collected from 338 COVID-19 patients grouped into 3 independent cohorts (Figure 1, Table 1, and Supplementary Data File 1).…”
Section: Introductionmentioning
confidence: 99%
“…). The NfL levels measured in HC and MS subgroups were previously reported (Masvekar R et al, 2021) and are used in the current study only as a positive control of neuronal injury; the measurements of other COVID-19 prognostic biomarkers in these control samples were not reported previously. 378 serum or plasma samples were collected from 338 COVID-19 patients grouped into 3 independent cohorts (Figure 1, Table 1, and Supplementary Data File 1).…”
Given the continued spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), early predictors of coronavirus disease 19 (COVID-19) mortality might improve patients outcomes. Increased levels of circulating neurofilament light chain (NfL), a biomarker of neuro-axonal injury, have been observed in patients with severe COVID-19. We investigated whether NfL provides non-redundant clinical value to previously identified predictors of COVID-19 mortality.
We measured serum or plasma NfL concentrations in a blinded fashion in 3 cohorts totaling 338 COVID-19 patients. In cohort 1, we found significantly elevated NfL levels only in critically ill COVID-19 patients compared to healthy controls. Longitudinal cohort 2 data showed that NfL is elevated late in the course of the disease, following two other prognostic markers of COVID-19: decrease in absolute lymphocyte count (ALC) and increase in lactate dehydrogenase (LDH). Significant correlations between LDH and ALC abnormalities and subsequent rise of NfL implicate multi-organ failure as a likely cause of neuronal injury at the later stages of COVID-19. Addition of NfL to age and gender in cohort 1 significantly improved the accuracy of mortality prediction and these improvements were validated in cohorts 2 and 3.
In conclusion, although substantial increase in serum/plasma NfL reproducibly enhances COVID-19 mortality prediction, NfL has clinically meaningful prognostic value only close to death, which may be too late to alter medical management. When combined with other prognostic biomarkers, rising longitudinal NfL measurements triggered by LDH and ALC abnormalities would identify patients at risk of COVID-19 associated mortality who might still benefit from escalated care.
“…As serum/plasma NfL levels increase with physiological aging (Disanto et al, 2017), the measured NfL concentrations were adjusted for effect of healthy aging as described previously (Masvekar R et al, 2021). Following age vs serum-or plasma-NfL equations from HC cohorts were used: ln(serum NfL) = 0.0177*Age + 0.9696 and ln(plasma NfL) = 0.0158*Age + 1.247.…”
Section: Adjustment For Effect Of Healthy Agingmentioning
confidence: 99%
“…Serum and plasma samples from healthy controls (HC) and multiple sclerosis (MS) subjects were collected at Neuroimmunological Diseases Section (NDS), NIAID after informed consent under IRB-approved protocol (ClinicalTrials.gov: NCT00794352). The NfL levels measured in HC and MS subgroups were previously reported (Masvekar R et al, 2021) and are used in the current study only as a positive control of neuronal injury; the measurements of other COVID-19 prognostic biomarkers in these control samples were not reported previously. 378 serum or plasma samples were collected from 338 COVID-19 patients grouped into 3 independent cohorts (Figure 1, Table 1, and Supplementary Data File 1).…”
Section: Introductionmentioning
confidence: 99%
“…). The NfL levels measured in HC and MS subgroups were previously reported (Masvekar R et al, 2021) and are used in the current study only as a positive control of neuronal injury; the measurements of other COVID-19 prognostic biomarkers in these control samples were not reported previously. 378 serum or plasma samples were collected from 338 COVID-19 patients grouped into 3 independent cohorts (Figure 1, Table 1, and Supplementary Data File 1).…”
Given the continued spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), early predictors of coronavirus disease 19 (COVID-19) mortality might improve patients outcomes. Increased levels of circulating neurofilament light chain (NfL), a biomarker of neuro-axonal injury, have been observed in patients with severe COVID-19. We investigated whether NfL provides non-redundant clinical value to previously identified predictors of COVID-19 mortality.
We measured serum or plasma NfL concentrations in a blinded fashion in 3 cohorts totaling 338 COVID-19 patients. In cohort 1, we found significantly elevated NfL levels only in critically ill COVID-19 patients compared to healthy controls. Longitudinal cohort 2 data showed that NfL is elevated late in the course of the disease, following two other prognostic markers of COVID-19: decrease in absolute lymphocyte count (ALC) and increase in lactate dehydrogenase (LDH). Significant correlations between LDH and ALC abnormalities and subsequent rise of NfL implicate multi-organ failure as a likely cause of neuronal injury at the later stages of COVID-19. Addition of NfL to age and gender in cohort 1 significantly improved the accuracy of mortality prediction and these improvements were validated in cohorts 2 and 3.
In conclusion, although substantial increase in serum/plasma NfL reproducibly enhances COVID-19 mortality prediction, NfL has clinically meaningful prognostic value only close to death, which may be too late to alter medical management. When combined with other prognostic biomarkers, rising longitudinal NfL measurements triggered by LDH and ALC abnormalities would identify patients at risk of COVID-19 associated mortality who might still benefit from escalated care.
“…Interestingly, another study found no correlation between baseline serum NfL levels and baseline EDSS. Interestingly, high baseline serum NfL concentrations predicted high EDSS levels at follow-up and at study end [ 318 ]. Not just flat concentrations, but the change in serum NfL levels has also been associated with a change in EDSS [ 306 , 310 ] scores.…”
Section: Fluid Biomarkers Of Multiple Sclerosismentioning
Introduction: Multiple Sclerosis (MS) is the most common immune-mediated chronic neurodegenerative disease of the central nervous system (CNS) affecting young people. This is due to the permanent disability, cognitive impairment, and the enormous detrimental impact MS can exert on a patient’s health-related quality of life. It is of great importance to recognise it in time and commence adequate treatment at an early stage. The currently used disease-modifying therapies (DMT) aim to reduce disease activity and thus halt disability development, which in current clinical practice are monitored by clinical and imaging parameters but not by biomarkers found in blood and/or the cerebrospinal fluid (CSF). Both clinical and radiological measures routinely used to monitor disease activity lack information on the fundamental pathophysiological features and mechanisms of MS. Furthermore, they lag behind the disease process itself. By the time a clinical relapse becomes evident or a new lesion appears on the MRI scan, potentially irreversible damage has already occurred in the CNS. In recent years, several biomarkers that previously have been linked to other neurological and immunological diseases have received increased attention in MS. Additionally, other novel, potential biomarkers with prognostic and diagnostic properties have been detected in the CSF and blood of MS patients. Areas covered: In this review, we summarise the most up-to-date knowledge and research conducted on the already known and most promising new biomarker candidates found in the CSF and blood of MS patients. Discussion: the current diagnostic criteria of MS relies on three pillars: MRI imaging, clinical events, and the presence of oligoclonal bands in the CSF (which was reinstated into the diagnostic criteria by the most recent revision). Even though the most recent McDonald criteria made the diagnosis of MS faster than the prior iteration, it is still not an infallible diagnostic toolset, especially at the very early stage of the clinically isolated syndrome. Together with the gold standard MRI and clinical measures, ancillary blood and CSF biomarkers may not just improve diagnostic accuracy and speed but very well may become agents to monitor therapeutic efficacy and make even more personalised treatment in MS a reality in the near future. The major disadvantage of these biomarkers in the past has been the need to obtain CSF to measure them. However, the recent advances in extremely sensitive immunoassays made their measurement possible from peripheral blood even when present only in minuscule concentrations. This should mark the beginning of a new biomarker research and utilisation era in MS.
“… 13 CSF biomarkers have also been identified for multiple sclerosis, Parkinson's disease and Alzheimers disease (AD). 14 , 15 AD is probably the most extensively studied, and in AD, the ratio of the content of amyloid beta peptide Aβ 42/ Aβ 40 has been used to predict cortical amyloid deposition. 16 Idiopathic normal pressure hydrocephalus (iNPH) may also show changes in Aβ 42 levels compared with age-matched controls.…”
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