Cerebrospinal fluid adenosine deaminase activity: A complimentary tool in the early diagnosis of tuberculous meningitis

Kashyap, Rajpal S.; Kainthla, Rani P; Mudaliar, Anju V; Purohit, Hemant J.; Taori, Girdhar M.; Daginawala, Hatim F.

doi:10.1186/1743-8454-3-5

Cited by 54 publications

(25 citation statements)

References 19 publications

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“…Levels of ADA in CSF are not only raised in TB meningitis, but also in conditions like other meningeal inflammation and certain intracranial tumors 7 . But it is a test to confirm etiology and has good predictive value 7 .…”

Section: Discussionmentioning

confidence: 99%

Cytomorphologic Analysis of Body Fluids

Bhade

Ukey

Chikhalikar

2019

mvpjms

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Background: Many etiologies result in development of effusions in the serous cavities. Of these pleural, peritoneal, pericardial fluids and cerebrospinal fluid are the most common. Cytologic study is done to diagnose disease ranging from benign (infective and inflammation) to malignant effusion 1 . Setting and Study Design: Pleural, peritoneal, and cerebrospinal fluid were included and others were excluded. In this study the significance of fluid cytology in the diagnosis of various neoplastic and non neoplastic lesions in the cytology section was carried out. Materials and Methods: All samples received in the Department of Pathology -pleural fluid, ascitic fluid and CSF were evaluated. Gross Examination of fluids was done for total Volume, Colour and Clarity. Biochemical test correlation of fluid Adenosine De-Aminase (ADA) levels was done wherever necessary. Diagnosis was done by consultants who were posted in cytopathology section. Results: In this prospective study from August 2015-2017, total 146 cases were studied of which 65 cases were of ascitic fluid, 41 of pleural fluid and 40 cases of CSF. Of these 146 cases, benign cases were 134 (91.78%), malignant effusion 9(6.16%) and suspicious of malignancy were 3 (2.05%). Among 146 study cases, 136 (93%) cases were having similar cytomorphological pattern (singly scattered), other 10 cases showed various patterns such as 3-dimensional clusters, sheets, etc. Conclusion: Body fluid examination with correlation of various parameters like thorough clinical history and examination, different serum marker levels, primary malignancy if present and previous cytological diagnosis are very useful for the final diagnosis. all the body fluids CSF examination with exfoliated malignant cells have worst prognosis. Cytologic examination of pleural, peritoneal, and pericardial effusions helps in diagnosing inflammatory conditions of the serous membranes, parasitic infestations,

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Section: Discussionmentioning

confidence: 99%

Cytomorphologic Analysis of Body Fluids

Bhade

Ukey

Chikhalikar

2019

mvpjms

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“…Adenosine deaminase (ADA) activity in the CSF of TBM patients has been suggested to be useful for early differential diagnosis of TBM [11]. The ADA activity of CSF and plasma have been evaluated as a diagnostic aid in TBM [12] and ADA activity in CSF was considered to be a simple, useful and rapid diagnostic test for early recognition of TBM in children [13]. However, overexpression of ADA was also often overexpressed in other forms of meningitis including infections with pyogenic bacteria [14].…”

Section: Introductionmentioning

confidence: 99%

Quantitative proteomics for identifying biomarkers for tuberculous meningitis

et al. 2012

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IntroductionTuberculous meningitis is a frequent extrapulmonary disease caused by Mycobacterium tuberculosis and is associated with high mortality rates and severe neurological sequelae. In an earlier study employing DNA microarrays, we had identified genes that were differentially expressed at the transcript level in human brain tissue from cases of tuberculous meningitis. In the current study, we used a quantitative proteomics approach to discover protein biomarkers for tuberculous meningitis.MethodsTo compare brain tissues from confirmed cased of tuberculous meningitis with uninfected brain tissue, we carried out quantitative protein expression profiling using iTRAQ labeling and LC-MS/MS analysis of SCX fractionated peptides on Agilent’s accurate mass QTOF mass spectrometer.Results and conclusionsThrough this approach, we identified both known and novel differentially regulated molecules. Those described previously included signal-regulatory protein alpha (SIRPA) and protein disulfide isomerase family A, member 6 (PDIA6), which have been shown to be overexpressed at the mRNA level in tuberculous meningitis. The novel overexpressed proteins identified in our study included amphiphysin (AMPH) and neurofascin (NFASC) while ferritin light chain (FTL) was found to be downregulated in TBM. We validated amphiphysin, neurofascin and ferritin light chain using immunohistochemistry which confirmed their differential expression in tuberculous meningitis. Overall, our data provides insights into the host response in tuberculous meningitis at the molecular level in addition to providing candidate diagnostic biomarkers for tuberculous meningitis.

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“…The indophenol method has been widely used to quantify urea after splitting it enzymatically with urease [ 33 ] and other substrates [ 34 , 35 ], and used in determining of deaminase activity [ 36 ]. This method can be easily adjusted to detect other metabolites or substances using a suitable deaminase.…”

Section: Discussionmentioning

confidence: 99%

An Enzymatic Assay for High-Throughput Screening of Cytidine-Producing Microbial Strains

Dong

Liu

et al. 2015

PLoS ONE

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Cytidine is an industrially useful precursor for the production of antiviral compounds and a variety of industrial compounds. Interest in the microbial production of cytidine has grown recently and high-throughput screening of cytidine over-producers is an important approach in large-scale industrial production using microorganisms. An enzymatic assay for cytidine was developed combining cytidine deaminase (CDA) and indophenol method. CDA catalyzes the cleavage of cytidine to uridine and NH3, the latter of which can be accurately determined using the indophenol method. The assay was performed in 96-well plates and had a linear detection range of cytidine of 0.058 - 10 mM. This assay was used to determine the amount of cytidine in fermentation flasks and the results were compared with that of High Perfomance Liquid Chromatography (HPLC) method. The detection range of the CDA method is not as wide as that of the HPLC, furthermore the correlation factor of CDA method is not as high as that of HPLC. However, it was suitable for the detection of large numbers of crude samples and was applied to high-throughput screening for high cytidine-producing strains using 96-well deep-hole culture plates. This assay was proved to be simple, accurate, specific and suitable for cytidine detection and high-throughput screening of cytidine-producing strains in large numbers of samples (96 well or more).

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Cerebrospinal fluid adenosine deaminase activity: A complimentary tool in the early diagnosis of tuberculous meningitis

Cited by 54 publications

References 19 publications

Cytomorphologic Analysis of Body Fluids

Cytomorphologic Analysis of Body Fluids

Quantitative proteomics for identifying biomarkers for tuberculous meningitis

An Enzymatic Assay for High-Throughput Screening of Cytidine-Producing Microbial Strains

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