Cerebroprotective Effect ofMoringa oleiferaagainst Focal Ischemic Stroke Induced by Middle Cerebral Artery Occlusion

Abstract

The protection against ischemic stroke is still required due to the limitation of therapeutic efficacy. Based on the role of oxidative stress in stroke pathophysiology, we determined whether Moringa oleifera, a plant possessing potent antioxidant activity, protected against brain damage and oxidative stress in animal model of focal stroke. M. oleifera leaves extract at doses of 100, 200 and 400 mg·kg−1 was orally given to male Wistar rats (300–350 g) once daily at a period of 2 weeks before the occlusion of right middle cerebral artery (Rt.MCAO) and 3 weeks after Rt.MCAO. The determinations of neurological score and temperature sensation were performed every 7 days throughout the study period, while the determinations of brain infarction volume, MDA level, and the activities of SOD, CAT, and GSH-Px were performed 24 hr after Rt.MCAO. The results showed that all doses of extract decreased infarction volume in both cortex and subcortex. The protective effect of medium and low doses of extract in all areas occurred mainly via the decreased oxidative stress. The protective effect of the high dose extract in striatum and hippocampus occurred via the same mechanism, whereas other mechanisms might play a crucial role in cortex. The detailed mechanism required further exploration.

Attenuating effect of α-pinene on neurobehavioural deficit, oxidative damage and inflammatory response following focal ischaemic stroke in rat

“…It has been shown that treatment by antioxidant and anti‐inflammatory agents has been able to impede sensorimotor dysfunction . In this regard, our findings indicate that the antioxidant and anti‐inflammatory activities of α‐pinene are attributed to attenuation of neuronal injury and improvement in neurobehavioural functioning.…”

“…The assessment of GPx activity was carried out by using Glutathione Peroxidase Assay Kit. GPx activity was measured according to a decrease in absorbance at 412 nm owing to the oxidation of NADPH to NADP + …”

Review of the Safety and Efficacy of Moringa oleifera

“…Kirisattayakul et al . () have demonstrated that a hydroalcohol extract of M . oleifera leaves at oral doses of 100–400 mg/kg for 3 weeks attenuated brain dysfunction and brain damage induced by cerebral ischemia.…”

An overview on neuroprotective effects of isothiocyanates for the treatment of neurodegenerative diseases

“…According to another study where CIR was induced by occlusion of middle cerebral artery occlusion (MCAO) in rats [80], it was demonstrated that M. oleifera leaf extract, when orally administered, protected against brain damage, by decreasing infarction volume in both cortex and subcortex regions and against oxidative stress, by modulating MDA level, and the activities of SOD, CAT, and glutathione peroxidase (GPx) [80].…”