Cerebroprotective effect of Eclipta alba against global model of cerebral ischemia induced oxidative stress in rats

Mansoorali, K.P.; Prakash, T.; Kotresha, D.; Prabhu, Krishnananda; Rao, N. Rama

doi:10.1016/j.phymed.2012.07.004

Cited by 50 publications

(38 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, the EtOAc extract could attenuate 6-OHDA-induced neuronal apoptosis by decreasing the Bax/Bcl-2 ratio and the caspase-3 activity, suggesting that the protective ef-fects of the EtOAc extract might be mainly due to its antioxidant property. Furthermore, previous studies showed that a hydroalcoholic extract of whole aerial parts of E. prostrata could prevent oxidative stressinduced ischaemic injury in rat brain by an elevation of antioxidant enzymes 21 . Another study reported that the ethanolic leaf extract expressed antioxidant capacity by augmenting catalase and glutathione activities, leading to a suppression of haloperidolinduced extrapyramidal movement disorders in the rats 33 .…”

Section: Discussionmentioning

confidence: 96%

“…Previous studies revealed that a butanol fraction of the whole plant could increase the level of acetylcholine and attenuate oxidative stress in the brain and serum of rats, suggesting its use to prevent memory impairment 20 . A hydroalcoholic extract obtained from the aerial parts markedly ameliorates ischaemiainduced neuronal loss of the rat brain 21 . Although E. prostrata has been reported to possess neuropharmacological profile, its neuroprotective effects against oxidative stress-induced pathogenesis of PD have not been evaluated.…”

Section: Introductionmentioning

confidence: 94%

See 1 more Smart Citation

Protective effects of ethyl acetate extract of Eclipta prostrata against 6-hydroxydopamine-induced neurotoxicity in SH-SY5Y cells

Jaisin¹,

Ratanachamnong²,

Prachayasittikul³

et al. 2016

ScienceAsia

View full text Add to dashboard Cite

ABSTRACT:Oxidative stress plays an important role in the pathogenesis of Parkinson's disease. The neurotoxin 6-hydroxydopamine initiates cellular oxidative stress which has been implicated in neurodegenerative conditions related to Parkinson's disease. Eclipta prostrata, also known as false daisy, is a plant belonging to the sunflower family. It is a rich source of phenolic compounds that confer the plant an antioxidant activity. In this study, we investigated the protective effects of ethyl acetate extract of E. prostrata against 6-hydroxydopamine-induced neurotoxicity in SH-SY5Y cells. Pretreatment with the ethyl acetate extract of E. prostrata before exposing the cells to 6-hydroxydopamine significantly increased cell survival and reduced intracellular reactive oxygen species formation in a concentrationdependent manner. In addition, the ethyl acetate extract of E. prostrata suppressed the Bax/Bcl-2 ratio and reduced caspase-3 activity, a key modulator of cell apoptosis. Our finding clearly shows that the ethyl acetate extract of E. prostrata possesses a potential antioxidant capacity and attenuates SH-SY5Y cell death by blocking apoptotic signalling caused by 6-hydroxydopamine. E. prostrata could therefore be used as a potential herb to prevent oxidative stress-induced neurodegeneration in Parkinson's disease.

show abstract

Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 94%

Protective effects of ethyl acetate extract of Eclipta prostrata against 6-hydroxydopamine-induced neurotoxicity in SH-SY5Y cells

Jaisin¹,

Ratanachamnong²,

Prachayasittikul³

et al. 2016

ScienceAsia

View full text Add to dashboard Cite

show abstract

“…quercetin was found to be sufficient for achieving cerebral protection in a cerebral I/R injury model; we also used quercetin at a dose of 20 mg/kg i.p. in our retinal I/R injury model (38) . Although, we could not assess the penetrance of quercetin into the retinal layers, we believe that when systemically used, quercetin may accumulate in the retinal layers at adequate concentrations during ischemic injury because of the possibly broken blood-retinal barrier.…”

Section: Discussionmentioning

confidence: 99%

Quercetin protects the retina by reducing apoptosis due to ischemia-reperfusion injury in a rat model

Arıkan¹,

Erşan²,

Karaca³

et al. 2015

Arquivos Brasileiros De Oftalmologia

View full text Add to dashboard Cite

Purpose: This study aimed to investigate the effect of quercetin on apoptotic cell death induced by ischemia-reperfusion (I/R) injury in the rat retina. Methods: Twenty-four rats were divided into four equal groups: control, ischemic, solvent, and quercetin. I/R injury was achieved by elevating the intraocular pressure above the perfusion pressure. Intraperitoneal injections of 20 mg/kg of quercetin and dimethyl sulfoxide (DMSO) were performed in the quercetin and solvent groups, respectively, immediately prior to I/R injury, and the researchers allowed for the retinas to be reperfused. Forty-eight hours after injury, the thicknesses of the retinal ganglion cell layer (RGCL), inner nuclear layer (INL), inner plexiform layer (IPL), outer plexiform layer (OPL), and outer nuclear layer (ONL) were measured in all groups. Moreover, the numbers of terminal deoxynucleotidyl transferase dUTP nick-end-labeled [TUNEL (+)] cells and caspase-3 (+) cells in both INL and ONL were evaluated in all groups. Results: The administration of quercetin was found to reduce the thinning of all retinal layers. The mean thickness of INL in the quercetin and ischemic groups was 21 ± 5.6 µm and 16 ± 6.4 µm, respectively (P<0.05). Similarly, the mean thickness of ONL in the quercetin and ischemic groups was 50 ± 12.8 µm and 40 ± 8.7 µm, respectively (P<0.05). The antiapoptotic effect of quercetin in terms of reducing the numbers of both TUNEL (+) cells and caspase-3 (+) cells was significant in INL. The mean number of TUNEL (+) cells in INL in the ischemic and quercetin groups was 476.8 ± 45.6/mm 2 and 238.72 ± 251/mm², respectively (P<0.005). The mean number of caspase-3 (+) cells in INL of ischemic and quercetin groups was 633.6 ± 38.7/mm 2 and 342.4 ± 36.1/mm 2 , respectively (P<0.001). Conclusion:The use of quercetin may be beneficial in the treatment of retinal I/R injury because of its antiapoptotic effect on the retinal layers, particularly in INL.

show abstract

“…31 The extract from E. prostrata has been reported to have cerebroprotective and antioxidative effects by reducing global cerebral ischemia in rats. 32 Taken together, the herbal formula B401 might have protective effects brought about by enhancing production of nitric oxide and VEGF, while reducing oxidative stress and inflammation. As for traditional Chinese medicine, we know that none of these herbs have much therapeutic value.…”

Section: Discussionmentioning

confidence: 99%

Oral treatment with herbal formula B401 alleviates penile toxicity in aging mice with manganism

Wu¹,

Hsu²,

Lin³

et al. 2015

OTT

View full text Add to dashboard Cite

Abstract:The present study aims to elucidate the roles of nitric oxide synthase activity, oxidative stress, inflammation, and apoptosis in penile toxicity of aging mice associated with excess manganese (Mn) treatment and to investigate the effect of oral treatment with the herbal formula B401 in this respect. ICR strain mice were divided into two groups: the vehicle (sham group) and the B401 (50 mg/kg) group. The mice were orally treated for 5 days; then a high single dose of MnCl 2 (100 mg/kg) was given by intraperitoneal injection to the mice. One day after MnCl 2 treatment, corpora cavernosal tissues of both Mn-treated mice and their controls were simultaneously sampled to examine their immunohistochemical staining and Western blot analysis. Nitric oxide (NO) production, levels of neuronal nitric oxide synthase (nNOS) and endothelial nitric oxide synthase (eNOS), expression levels of factors governing angiogenesis (vascular endothelial growth factor), oxidative stress (catalase, superoxide dismutase 2,4-hydroxynonenal), inflammation (tumor necrosis factor alpha), apoptosis (B-cell lymphoma 2 [Bcl-2], Bcl-2-associated X protein [Bax], cleaved poly(adenosine diphosphateribose) polymerase [c-PARP], cytochrome C, caspase-12, and caspase-3) were evaluated in penile corpus cavernosum of the mice. We found that penile toxicity in the mice was enhanced under excess Mn treatment through reduction of NOS activity and increase in oxidative stress, inflammation, and apoptosis in the penile cavernous tissue. Furthermore, the penile toxicity in mice with manganism was alleviated by oral B401 treatment through enhancement of both nitric oxide synthesis and angiogenesis, with simultaneous reduction of oxidative stress, inflammation, and apoptosis in penile corpus cavernosum. We suggest that the herbal formula B401 may serve as a potential dietotherapeutic supplement for penile toxicity or dysfunction in aging males.

show abstract

Cerebroprotective effect of Eclipta alba against global model of cerebral ischemia induced oxidative stress in rats

Cited by 50 publications

References 34 publications

Protective effects of ethyl acetate extract of Eclipta prostrata against 6-hydroxydopamine-induced neurotoxicity in SH-SY5Y cells

Protective effects of ethyl acetate extract of Eclipta prostrata against 6-hydroxydopamine-induced neurotoxicity in SH-SY5Y cells

Quercetin protects the retina by reducing apoptosis due to ischemia-reperfusion injury in a rat model

Oral treatment with herbal formula B401 alleviates penile toxicity in aging mice with manganism

Contact Info

Product

Resources

About