Cerebellar cortex of rat and other animals. A structural and ultrastructural study

Abstract

Cerebellar cortex was studied in 100 rats and other animals, 15-30 days old, using Golgi, intravitam methylene blue, and ultrastructural methods. Marchi and Nauta provided certain controls. Degeneration of afferent terminals was sought following lesions of pons and olive.In normal material, emphasis was upon the chanqing field relations between folial summit, side wall, and sulcus. Many previous findings of Fox, Ramon y Cajal and Snider were confirmed, and the effort made to reconcile departures from characteristic axonal and dendritic configuration with cytoarchitectonic pattern. Mossy and climbing terminals were examined in normal and degenerated states, using Golgi and ultrastructural material. Climbing fibers in Golgi are described in detail, including variations in course and sites of branchings. Mossy degeneration was found 3-5 days after lesions of both pons and olive. The clearest evidence thereof was provided in ultrastructural material. Minor evidences of degeneration were also found in climbing fiber strands in both materials, but no gross degeneration was encountered. Corresponding Golgi material was used for visualizing intact fibers in the face of adequate lesions.Ultrastructure also provided evidence concerning the axon cap surround, the initial segment of the Purkinje axon, comparison with these segmests in other cell types, and cross-identification of synapses between axon types and the different cell profiles of Golgi material.

Calcium-binding proteins in the cerebellar cortex of the bottlenose dolphin and harbour porpoise

“…In the present study, no CR-positive Lugaro cells were found. Lugaro cells have been found in all mammalian species studied (Fox, 1959;O'Leary et al, 1968;Palay and Chan-Palay, 1974;Braak and Braak, 1983;Laine and Axelrad, 1996). Assuming that these cells are also present in the cetaceans presently studied, one may suggest that they are CR-negative.…”

Comparative neuronal morphology of the cerebellar cortex in afrotherians, carnivores, cetartiodactyls, and primates

“…Histologically, cerebellar cortex exhibits a generally trilaminate architecture, which is similar in birds and mammals (Ramón y Cajal, 1909, 1911; Iwaniuk et al, 2006; Sultan and Glickstein, 2007). Whereas limited aspects of cerebellar neuron morphology have been described in some vertebrate species (e.g., mormyrid electric fish: Han et al, 2006; teleost fish: Murakami and Morita, 1987; alligator: Nicholson and Llinas, 1971; cat: Melik-Musyan and Fanardzhyan, 2004; duck: O'Leary et al, 1968; dolphin: Adanina, 1965; rhesus monkey: Fox et al, 1967; Rakic, 1972; human: Braak and Braak, 1983), the most detailed research has focused on rodents. In particular, Palay and Chan-Palay (1974) provided a comprehensive examination of the cerebellar cortex of the rat, documenting organizational features, neuronal morphology, and ultrastructure at the electron microscopic level.…”

Three‐dimensional morphology of cerebellar climbing fibers. A study by means of confocal laser scanning microscopy and scanning electron microscopy

“…Light and transmission electron microscopic studies of vertebrate cerebellar cortex (Carrea et al 1947;ChanPalay andPalay 1970, 1971;Fox et al 1969;Szentagothai 1966, 1980;Ito 1984;Larramendi and Victor 1967;Murphy et al 1973;O'Leary et al 1968O'Leary et al , 1971Palay and Chan-Palay 1974;Rivera-Domínguez et al 1974;Scheibel and Scheibel 1954;Szentagothai and Rajkovits 1959) have supported and extended the basic morphologic aspects of climbing fibers as reported more than one hundred years ago, in the pioneering and classical account of Ramón y Cajal (1888, 1955.…”