Cercosporin-bioinspired selective photooxidation reactions under mild conditions

Li, Jia; Bao, Wenhao; Tang, Zhaocheng; Guo, Baodang; Zhang, Shiwei; Liu, Haili; Huang, Shuping; Zhang, Yan; Rao, Yijian

doi:10.1039/c9gc02270h

Cited by 49 publications

(43 citation statements)

References 137 publications

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“…Considering the limited production of CP on PDA plate [ 22 ], S-7 culture medium was firstly chosen as the basic medium to optimize the liquid fermentation conditions [ 19 , 22 , 34 ], including culture time, medium pH, temperature, carbon source and nitrogen source (Fig. 1 b–f).…”

Section: Resultsmentioning

confidence: 99%

“…CP is also a potent inhibitor of protein kinase C (PKC) [ 18 ], which regulates numerous intracellular signal transduction, including cell differentiation, cell proliferation and inflammatory response, by controlling the function of other proteins through phosphorylation. More recently, we have developed CP as a new class of metal-free photocatalyst to catalyze a series of chemical transformations, including selective photo-oxidation, C–H activation, C–N coupling and C–S coupling [ 19 – 23 ]. Meanwhile, it has also been utilized to fabricate a novel HARCP/HAp photocatalyst (HexaAcetyl-Reductive Cercosporin/Hydroxyapatite) after a simple structural modification, allowing to efficiently photoremove tetracycline in water pollution under natural sunlight [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

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Enhanced cercosporin production by co-culturing Cercospora sp. JNU001 with leaf-spot-disease-related endophytic bacteria

Zhou

et al. 2021

Microb Cell Fact

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Background Owing to the excellent properties of photosensitization, cercosporin, one of naturally occurring perylenequinonoid pigments, has been widely used in photodynamic therapy, or as an antimicrobial agent and an organophotocatalyst. However, because of low efficiency of total chemical synthesis and low yield of current microbial fermentation, the limited production restricts its broad applications. Thus, the strategies to improve the production of cercosporin were highly desired. Besides traditional optimization methods, here we screened leaf-spot-disease-related endophytic bacteria to co-culture with our previous identified Cercospora sp. JNU001 to increase cercosporin production. Results Bacillus velezensis B04 and Lysinibacillus sp. B15 isolated from leaves with leaf spot diseases were found to facilitate cercosporin secretion into the broth and then enhance the production of cercosporin. After 4 days of co-culture, Bacillus velezensis B04 allowed to increase the production of cercosporin from 128.2 mg/L to 984.4 mg/L, which was 7.68-fold of the previously reported one. Lysinibacillus sp. B15 could also enhance the production of cercosporin with a yield of 626.3 mg/L, which was 4.89-fold higher than the starting condition. More importantly, we found that bacteria B04 and B15 employed two different mechanisms to improve the production of cercosporin, in which B04 facilitated cercosporin secretion into the broth by loosening and damaging the hyphae surface of Cercospora sp. JNU001 while B15 could adsorb cercosporin to improve its secretion. Conclusions We here established a novel and effective co-culture method to improve the production of cercosporin by increasing its secretion ability from Cercospora sp. JNU001, allowing to develop more potential applications of cercosporin.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhanced cercosporin production by co-culturing Cercospora sp. JNU001 with leaf-spot-disease-related endophytic bacteria

Zhou

et al. 2021

Microb Cell Fact

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“…Considering the limited production of cercosporin (CP) on PDA plate [22], S-7 culture medium was rstly chosen as the basic medium to optimize the liquid fermentation conditions [19,22,34], including culture time, medium pH, temperature, carbon source and nitrogen source ( Fig. 1B-F).…”

Section: Determination and Optimization Of Liquid Fermentation Conditmentioning

confidence: 99%

“…Cercosporin is also a potent inhibitor of protein kinase C (PKC) [18], which regulates numerous intracellular signal transduction, including cell differentiation, cell proliferation and in ammatory response, by controlling the function of other proteins through the phosphorylation. More recently, we have developed cercosporin as a new class of metal-free photocatalyst to catalyze a series of chemical transformations, including selective photxidation, C-H activation, C-N coupling and C-S coupling [19][20][21][22][23]. Meanwhile, it has also been utilized to fabricate a novel HARCP/HAp photocatalyst (HexaAcetyl-Reductive Cercosporin/Hydroxyapatite) after a simple structural modi cation, allowing to e ciently photoremove tetracycline in water pollution under natural sunlight [24].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Cercosporin Production by Co-culturing Cercospora Sp. JNU001 With Leaf-Spot-Disease-Related Endophytic Bacteria

Zhou

et al. 2021

Preprint

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Background: Owing to the excellent properties of photosensitization, cercosporin, one of naturally occurring perylenequinonoid pigments, has been widely used in photodynamic therapy, or as an antimicrobial agent and an organophotocatalyst. However, because of low efficiency of total chemical synthesis and low yield of current microbial fermentation, the limited production restricts its broad applications. Thus, the strategies to improve the production of cercosporin were highly desired. Besides traditional optimization methods, here we screened leaf-spot-disease-related endophytic bacteria to co-culture with our previous identified Cercospora sp. JNU001 to increase cercosporin production. Results: Bacillus velezensis B04 and Lysinibacillus sp. B15 isolated from leaves with leaf spot diseases were found to facilitate cercosporin secretion into the broth and then enhance the production of cercosporin. After 4 days of co-culture, Bacillus velezensis B04 allowed to increase the production of cercosporin from 128.2 mg/L to 984.4 mg/L, which was 7.68-fold of the previously reported one. Lysinibacillus sp. B15 could also enhance the production of cercosporin with a yield of 626.3 mg/L, which was 4.89-fold higher than the starting condition. More importantly, we found that bacteria B04 and B15 employed two different mechanisms to improve the production of cercosporin, in which B04 facilitated cercosporin secretion into the broth by loosening and damaging the hyphae surface of Cercospora sp. JNU001 while B15 could absorb cercosporin to improve its secretion.Conclusions: We here established a novel and effective co-culture method to improve the production of cercosporin by increasing its secretion ability from Cercospora sp. JNU001, allowing to develop more potential applications of cercosporin.

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Emodin as a novel organic photocatalyst for selective oxidation of sulfides under mild conditions

et al. 2020

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Cercosporin-bioinspired selective photooxidation reactions under mild conditions

Abstract: Cercosporin was developed as a green and efficient organophotocatalyst for a wide range of selective oxidations with reactive oxygen species.

Cited by 49 publications

References 137 publications

Enhanced cercosporin production by co-culturing Cercospora sp. JNU001 with leaf-spot-disease-related endophytic bacteria

Enhanced cercosporin production by co-culturing Cercospora sp. JNU001 with leaf-spot-disease-related endophytic bacteria

Enhanced Cercosporin Production by Co-culturing Cercospora Sp. JNU001 With Leaf-Spot-Disease-Related Endophytic Bacteria

Emodin as a novel organic photocatalyst for selective oxidation of sulfides under mild conditions

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Cercosporin-bioinspired selective photooxidation reactions under mild conditions

Abstract: Cercosporin was developed as a green and efficient organophotocatalyst for a wide range of selective oxidations with reactive oxygen species.

Cited by 49 publications

References 137 publications

Enhanced cercosporin production by co-culturing Cercospora sp. JNU001 with leaf-spot-disease-related endophytic bacteria

Enhanced cercosporin production by co-culturing Cercospora sp. JNU001 with leaf-spot-disease-related endophytic bacteria

Enhanced Cercosporin Production by Co-culturing Cercospora Sp. JNU001&nbsp;With Leaf-Spot-Disease-Related Endophytic Bacteria

Emodin as a novel organic photocatalyst for selective oxidation of sulfides under mild conditions

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Enhanced Cercosporin Production by Co-culturing Cercospora Sp. JNU001 With Leaf-Spot-Disease-Related Endophytic Bacteria