Cercosporin-bioinspired photoinactivation of harmful cyanobacteria under natural sunlight via bifunctional mechanisms

Liu, Meiling; Zhang, Yan; Yuan, Zhenbo; Lu, Liushen; Liu, Xuanzhong; Zhu, Xiaonan; Wang, Lingling; Liu, Chang‐Mei; Rao, Yijian

doi:10.1016/j.watres.2022.118242

Cited by 9 publications

(3 citation statements)

References 51 publications

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“…On the other hand, organic photosensitizers, such as chlorin e6, riboflavin, and erythrosine B, are well known for their low cost and low toxicity but are less used for photoinactivation of cyanobacteria. 18,19 Probably, due to rapid photobleaching, organic PSs cannot continuously produce reactive oxygen species (ROS) for degradation of algae under long-term irradiation. 20 Therefore, there is a pressing need for an economic and environmentally friendly photosensitizer that is capable of both highly efficient cyanobacterial inactivation and minimal secondary pollution.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Inorganic photosensitizers (PSs), such as metal oxides (e.g., TiO 2 and Ag 2 O), , metal salt (e.g., AgPO 4 ), and g-C 3 N 4 , , are famous for their high efficiency in algae degradation but are typically disposed in a relatively high dosage and relatively difficult to be recycled (Table S1). On the other hand, organic photosensitizers, such as chlorin e6, riboflavin, and erythrosine B, are well known for their low cost and low toxicity but are less used for photoinactivation of cyanobacteria. , Probably, due to rapid photobleaching, organic PSs cannot continuously produce reactive oxygen species (ROS) for degradation of algae under long-term irradiation . Therefore, there is a pressing need for an economic and environmentally friendly photosensitizer that is capable of both highly efficient cyanobacterial inactivation and minimal secondary pollution.…”

Section: Introductionmentioning

confidence: 99%

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Self-Immolative Polythiophene for Sunlight Inactivation of Harmful Cyanobacteria

Lang,

Wang,

Zhou

et al. 2023

Environ. Sci. Technol.

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Harmful cyanobacterial blooms and the released microcystins (MCs) caused serious environmental and public health concerns to drinking water safety. Photo-oxidation is an appealing treatment option and alternative to conventional flocculation and microbial antagonists, but the performances of current photosensitizers (either inorganic or organic) are unsatisfactory. Here, a polythiophene photosensitizer (PT10) with both high yield of reactive oxygen species (ROS) production (mainly 1O2, ΦΔ = 0.51, > 8 h continuous generation) and moderate photostability was used as a powerful algaecide to inhibit Microcystis aeruginosa. Due to the positive charge of PT10, the algal cells were quickly flocculated, followed by efficient inactivation in 4 h under white light irradiation (96.7%, 10 mW/cm2). Meanwhile, PT10 was self-immolated in about 6 h. Upon biosafety evaluation with adult zebrafish, the low toxicity of PT10 and the degradation products of PT10 and algae (early logarithmic growth stage) were confirmed. In addition, microcystin-LR (MC-LR), a toxic microcystin that will be released during the destruction of the algal cells, was also degraded. Therefore, PT10-based photoinactivation of M. aeruginosa featured both high performance and low secondary pollution. In real-world aquatic systems, PT10 was confirmed to be capable of sunlight-assisted inactivation of M. aeruginosa and prevent algal blooms, thus making it appealing for environmental remediation.

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Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Self-Immolative Polythiophene for Sunlight Inactivation of Harmful Cyanobacteria

Lang,

Wang,

Zhou

et al. 2023

Environ. Sci. Technol.

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“…Chemical agents such as triosyn, hydrogen peroxide, and copper sulfate were found competent in a small period of application [11]. In recent years, studies have reported that certain natural products can efficiently degrade cyanobacteria, such 2 of 11 as cercosporin and sophorolipids (SLs) [12][13][14]. Compared with other agents, SLs exhibit remarkable advantages in dealing with large-scale CyanoHABs.…”

Section: Introductionmentioning

confidence: 99%

Efficient Biosynthesis of Acidic/Lactonic Sophorolipids and Their Application in the Remediation of Cyanobacterial Harmful Algal Blooms

Xia

Shi

Chu

et al. 2023

IJMS

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Cyanobacterial harmful algal blooms (CyanoHABs) pose significant threats to human health and natural ecosystems worldwide, primarily caused by water eutrophication, increased surface water temperature, and co-occurring microorganisms. Urgent action is needed to develop an eco-friendly solution to effectively curb the proliferation of CyanoHABs. Sophorolipids (SLs) are fully biodegradable biosurfactants synthesized by Starmerella bombicola. They can be classified into lactone and acid types. The lactone type displays strong antimicrobial activity, while the acid type exhibits good solubility, which make them ideal agents for mitigating CyanoHABs. Nevertheless, the broad utilization of SLs are hindered by their expensive production costs and the absence of effective genetic editing tools in the native host. In this study, we constructed recombinant strains capable of producing either acidic or lactonic SLs using the CRISPR-Cas9 gene editing system. The yields of acidic and lactonic SLs reached 53.64 g/L and 45.32 g/L in a shaking flask, respectively. In a 5 L fermenter, acidic SLs reached 129.7 g/L using low-cost glucose and rapeseed oil as substrates. The addition of 5 mg/L lactonic SLs effectively degraded cyanobacteria within 30 min, and a ratio of 8.25:1.75 of lactonic to acidic SLs showed the highest degradation efficiency. This study offers a safe and promising solution for CyanoHABs treatment.

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Synthetic Biology‐based Construction of Unnatural Perylenequinones with Improved Photodynamic Anticancer Activities

Su,

Guo,

et al. 2024

Angewandte Chemie

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The construction of structural complexity and diversity of natural products is crucial for drug discovery and development. To overcome high dark toxicity and poor photostability of natural photosensitizer perylenequinones (PQs) for photodynamic therapy, herein, we aim to introduce the structural complexity and diversity to biosynthesize the desired unnatural PQs in fungus Cercospora through synthetic biology‐based strategy. Thus, we first elucidate the intricate biosynthetic pathways of class B PQs and reveal how the branching enzymes create their structural complexity and diversity from a common ancestor. This enables the rational reprogramming of cercosporin biosynthetic pathway in Cercospora to generate diverse unnatural PQs without chemical modification. Among them, unnatural cercosporin A displays remarkably low dark toxicity and high photostability with retention of great photodynamic anticancer and antimicrobial activities. Moreover, it is found that, unlike cercosporin, the unnatural cercosporin A could be selectively accumulated in cancer cells, providing potential targets for drug development. Therefore, this work provides a comprehensive foundation for preparing unnatural products with customized functions through synthetic biology‐based strategies, thus facilitating drug discovery pipelines from nature.

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Cercosporin-bioinspired photoinactivation of harmful cyanobacteria under natural sunlight via bifunctional mechanisms

Cited by 9 publications

References 51 publications

Self-Immolative Polythiophene for Sunlight Inactivation of Harmful Cyanobacteria

Self-Immolative Polythiophene for Sunlight Inactivation of Harmful Cyanobacteria

Efficient Biosynthesis of Acidic/Lactonic Sophorolipids and Their Application in the Remediation of Cyanobacterial Harmful Algal Blooms

Synthetic Biology‐based Construction of Unnatural Perylenequinones with Improved Photodynamic Anticancer Activities

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