Cercospora cf. nicotianae is a causal agent of Cercospora leaf blight of soybean

Sautua, Francisco; Searight, Jacob; Doyle, Vinson P.; Scandiani, María Mercedes; Carmona, Marcelo

doi:10.1007/s10658-020-01969-z

Cited by 17 publications

(11 citation statements)

References 10 publications

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“…The methodology was carried out according to Sautua et al . (2020). Conidial suspensions (10 3 conidia/ml) from each isolate grown on soybean leaf decoction agar (Sautua et al ., 2020) were sprayed on whole plants at the beginning seed stage (R5 stage; Fehr and Caviness, 1977).…”

Section: Methodsmentioning

confidence: 99%

“…(2020). Conidial suspensions (10 3 conidia/ml) from each isolate grown on soybean leaf decoction agar (Sautua et al ., 2020) were sprayed on whole plants at the beginning seed stage (R5 stage; Fehr and Caviness, 1977). Spore suspensions were prepared by collecting spores from 7‐day‐old colonies in sterile water, with 1 ml Tween 20 (0.2%) added to facilitate the dispersal of conidia.…”

Section: Methodsmentioning

confidence: 99%

“…cf. nicotianae was identified as one of several species in association with CLB in Bolivia (Sautua et al ., 2019a), and has been confirmed for the first time to be a causal agent of CLB on soybean (Sautua et al ., 2020). However, these studies focused on a limited number of isolates not representative of the major soybean‐growing regions in Argentina, one of the largest soybean‐producing countries in South America.…”

Section: Introductionmentioning

confidence: 99%

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Fungicide resistance in Cercospora species causing cercospora leaf blight and purple seed stain of soybean in Argentina

et al. 2020

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Cercospora species cause cercospora leaf blight (CLB) and purple seed stain (PSS) on soybean. Because there are few resistant soybean varieties available, CLB/PSS management relies heavily upon fungicide applications. Sensitivity of 62 Argentinian Cercospora isolates to demethylation inhibitor (DMI), methyl benzimidazole carbamate (MBC), quinone outside inhibitor (QoI), succinate dehydrogenase inhibitor (SDHI) fungicides, and mancozeb was determined in this study. All isolates were sensitive to difenoconazole, epoxiconazole, prothioconazole, tebuconazole, and cyproconazole (EC50 values ranged from 0.006 to 2.4 µg/ml). In contrast, 51% of the tested isolates were sensitive (EC50 values ranged from 0.003 to 0.2 µg/ml), and 49% were highly resistant (EC50 > 100 µg/ml) to carbendazim. Interestingly, all isolates were completely resistant to azoxystrobin, trifloxystrobin, and pyraclostrobin, and insensitive to boscalid, fluxapyroxad, and pydiflumetofen (EC50 > 100 µg/ml). The G143A mutation was detected in 82% (53) of the QoI‐resistant isolates and the E198A mutation in 97% (31) of the carbendazim‐resistant isolates. No apparent resistance mutations were detected in the succinate dehydrogenase genes (subunits sdhB, sdhC, and sdhD). Mancozeb completely inhibited mycelial growth of the isolates evaluated at a concentration of 100 µg/ml. All Argentinian Cercospora isolates were sensitive to the DMI fungicides tested, but we report for the first time resistance to QoI and MBC fungicides. Mechanism(s) other than fungicide target‐site modification may be responsible for resistance of Cercospora to QoI and MBC fungicides. Moreover, based on our results and on the recent introduction of SDHI fungicides on soybean in Argentina, Cercospora species causing CLB/PSS are insensitive (naturally resistant) to SDHI fungicides. Insensitivity must be confirmed under field conditions.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fungicide resistance in Cercospora species causing cercospora leaf blight and purple seed stain of soybean in Argentina

et al. 2020

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“…cf. nicotianae ( Sautua et al 2020 ) can also cause soybean leaf blight. Moreover, some cryptic species of Cercospora infect soybean ( Soares et al 2015 ).…”

Section: Introductionmentioning

confidence: 99%

High-quality genome assembly of the soybean fungal pathogen Cercospora kikuchii

Kashiwa

Suzuki

2021

G3 Genes|Genomes|Genetics

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Plant diseases caused by the Cercospora genus of ascomycete fungi are a major concern for commercial agricultural practices. Several Cercospora species can affect soybeans, such as C. kikuchii which causes soybean leaf blight. Speciation in Cercospora on soybean has not been adequately studied. Some cryptic groups of Cercospora also cause diseases on soybean. Moreover, it has been known C. kikuchii population genetic structure is different between countries. Consequently, further genomic information could help to elucidate the covert differentiation of Cercospora diseases in soybean. Here, we report for the first time, a chromosome-level genome assembly for C. kikuchii. The genome assembly of 9 contigs was 34.44 Mb and the N50 was 4.19 Mb. Based on ab-initio gene prediction, several pathogenicity-related genes, including 242 genes for effector candidates, 55 secondary metabolite gene clusters, and 399 carbohydrate-active enzyme genes were identified. The genome sequence and the features described in this study provide a solid foundation for comparative and evolutionary genomic analysis for Cercospora species that cause soybean diseases worldwide.

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“…Estas manchas se asemejaron a la mancha ojo de rana de la soja causada por Cercospora sojina Hara nicotianae, previamente asociado con el TH (Sautua et al, 2019a), es un agente causante del TH de la soja (Sautua et al, 2020a).…”

Section: Análisis Filogenético De Aislados Bolivianosunclassified

Sensibilidad y resistencia a fungicidas de <i>Cercospora kikuchii</i>, agente causal del tizón de la hoja y mancha púrpura de la semilla de la soja

Sautua¹

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En Argentina, diferentes especies de Cercospora causan el tizón de la hoja (TH) y la mancha púrpura de la semilla (MPS) en soja. Debido a que no hay variedades de soja resistentes disponibles en el mercado, el manejo de TH/MPS depende en gran medida de aplicaciones de fungicidas. En este estudio se determinó la sensibilidad de 62 cepas de Cercospora argentinas a fungicidas inhibidores de la desmetilación (DMI), carbamato de metilbenzimidazol (MBC), inhibidores de la quinona externa (QoI), inhibidores de la succinato deshidrogenasa (SDHI) y mancozeb. Todos los aislados fueron sensibles a difenoconazole, epoxiconazole, protioconazole, tebuconazole y cyproconazole (los valores de EC50 variaron de 0,006 a 2,4 µg mL-1). Por el contrario, la mitad (51%) de los aislados fueron sensibles (los valores de EC50 variaron de 0,003 a 0,2 µg mL-1) y la mitad (49%) altamente resistentes (EC50> 100 µg mL-1) a carbendazim. Todos los aislados fueron completamente resistentes a azoxystrobina, trifloxystrobina, pyraclostrobina e insensibles a boscalid, fluxapyroxad y pydiflumetofen (EC50 > 100 µg mL-1). La mutación G143A se detectó en el 82% de los aislados resistentes a QoI y la mutación E198A en el 97% de los aislados resistentes a carbendazim. No se detectaron mutaciones de resistencia aparentes en los genes que codifican para las subunidades B, C y D de la succinato deshidrogenasa. Mancozeb inhibió completamente el crecimiento micelial de los aislados evaluados a 100 µg mL-1. Todos los aislados argentinos de Cercospora fueron sensibles a los fungicidas DMI evaluados, pero se reporta por primera vez resistencia a los fungicidas QoI y MBC. Otro mecanismo(s) además de la modificación del sitio objeto de control del fungicida podrían ser responsable(s) de la resistencia de Cercospora a los fungicidas QoI y MBC. Además, debido a la reciente introducción de fungicidas SDHI en soja en Argentina, se especula que las especies de Cercospora que causan TH/MPS son insensibles (naturalmente resistentes) a este grupo de fungicidas. La insensibilidad debe confirmarse en condiciones de campo.

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Cercospora cf. nicotianae is a causal agent of Cercospora leaf blight of soybean

Cited by 17 publications

References 10 publications

Fungicide resistance in Cercospora species causing cercospora leaf blight and purple seed stain of soybean in Argentina

Fungicide resistance in Cercospora species causing cercospora leaf blight and purple seed stain of soybean in Argentina

High-quality genome assembly of the soybean fungal pathogen Cercospora kikuchii

Sensibilidad y resistencia a fungicidas de <i>Cercospora kikuchii</i>, agente causal del tizón de la hoja y mancha púrpura de la semilla de la soja

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