“…When using various replacement materials, the details of bone defect restoration depend both on the material itself and on the experimental model: the type of animal, the localization of the experimental bone defect, etc. [ 21 – 23 ]. As a rule, histological studies of interaction between materials and bone tissue are intended to assess inflammatory reactions, severity of vascularization, formation, and maturation of new bone taking into account the area around the implants [ 24 ].…”
The aim of the study
is to evaluate biocompatibility of a novel hybrid polyoligomer in
in vitro
and
in vivo
models.
Materials and Methods.
Cytotoxicity of the material was investigated using the MTT assay with human dermal fibroblasts as test cultures. To study direct interaction of the hybrid polyoligomer with cells, the fibroblasts were cultured on the polymer samples for 96 h, the cultures were assessed every 24 h using fluorescence microscopy. To study the tissue reaction in the area of contact with the donor bed and the morphological features of the implanted sample restructuring, a case-control study was performed using a rabbit model. Samples of hybrid polyoligomer were implanted into the bone defect formed in the left iliac crest in 10 rabbits. In the control group, the prepared allograft samples were transplanted into similar defects in 10 animals. The rabbits were sacrificed 4 and 8 weeks after the operation. The standard morphological methods with hematoxylin and eosin staining and immunohistochemical Ki-67 proliferation marker evaluation were used to assess the state of tissues in the defect area.
Results.
The results demonstrate that the hybrid polyoligomer is not cytotoxic (cytotoxicity score 0–1), cells adhere well to its surface, retain their viability and typical morphology throughout the entire observation period. No negative impact of material implantation on the health state and behavior of animals was detected. Morphological examination showed the absence of inflammatory changes, formation of thin-walled capillary vessels, and considerable proliferative activity of mesenchymal cells in the defect area, even though it was more intense than in the control group.
Conclusion.
No inflammation signs were detected by 8
th
week of the experiment. It was defined that new bone was beginning to form. The results of analysis support the conclusion that the developed hybrid materials are prospective for further research as potential bone substitute.
“…When using various replacement materials, the details of bone defect restoration depend both on the material itself and on the experimental model: the type of animal, the localization of the experimental bone defect, etc. [ 21 – 23 ]. As a rule, histological studies of interaction between materials and bone tissue are intended to assess inflammatory reactions, severity of vascularization, formation, and maturation of new bone taking into account the area around the implants [ 24 ].…”
The aim of the study
is to evaluate biocompatibility of a novel hybrid polyoligomer in
in vitro
and
in vivo
models.
Materials and Methods.
Cytotoxicity of the material was investigated using the MTT assay with human dermal fibroblasts as test cultures. To study direct interaction of the hybrid polyoligomer with cells, the fibroblasts were cultured on the polymer samples for 96 h, the cultures were assessed every 24 h using fluorescence microscopy. To study the tissue reaction in the area of contact with the donor bed and the morphological features of the implanted sample restructuring, a case-control study was performed using a rabbit model. Samples of hybrid polyoligomer were implanted into the bone defect formed in the left iliac crest in 10 rabbits. In the control group, the prepared allograft samples were transplanted into similar defects in 10 animals. The rabbits were sacrificed 4 and 8 weeks after the operation. The standard morphological methods with hematoxylin and eosin staining and immunohistochemical Ki-67 proliferation marker evaluation were used to assess the state of tissues in the defect area.
Results.
The results demonstrate that the hybrid polyoligomer is not cytotoxic (cytotoxicity score 0–1), cells adhere well to its surface, retain their viability and typical morphology throughout the entire observation period. No negative impact of material implantation on the health state and behavior of animals was detected. Morphological examination showed the absence of inflammatory changes, formation of thin-walled capillary vessels, and considerable proliferative activity of mesenchymal cells in the defect area, even though it was more intense than in the control group.
Conclusion.
No inflammation signs were detected by 8
th
week of the experiment. It was defined that new bone was beginning to form. The results of analysis support the conclusion that the developed hybrid materials are prospective for further research as potential bone substitute.
“…Therefore, the use of PLA in the development of the hybrid polymer allowed us to increase the mechanical strength of the framework, while ensuring partial, gradual biodegradation of the material, and, when necessary, the possibility of functionalizing the material with various substances, for example, to ensure long-term antibacterial activity [ 24 ]. PLA offers some advantages, including availability, low cost, and applicability for 3D printing, as demonstrated in a number of previous studies on trabecular bone replacement scaffolds [ 39 , 56 , 57 , 58 ].…”
Section: Discussionmentioning
confidence: 99%
“…When studying the peculiarities of the bone defect restoration using materials intended for bone grafting in experimental models, one should consider that these complex processes depend both on the material and on the experimental model: for example, the animal type and the location of the bone defect [ 58 , 59 , 60 ].…”
This paper provides a study of two bone substitutes: a hybrid porous polymer and an osteoplastic matrix based on a bovine-derived xenograft. Both materials are porous, but their pore characteristics are different. The osteoplastic matrix has pores of 300–600 µm and the hybrid polymer has smaller pores, generally of 6–20 µm, but with some pores up to 100 µm across. SEM data confirmed the porometry results and demonstrated the different structures of the materials. Therefore, both materials were characterized by an interconnected porous structure and provided conditions for the adhesion and vital activity of human ASCs in vitro. In an experimental model of rabbit shin bone defect, it was shown that, during the 6-month observation period, neither of the materials caused negative reactions in the experimental animals. By the end of the observation period, restoration of the defects in animals in both groups was completed, and elements of both materials were preserved in the defect areas. Data from morphological examinations and CT data demonstrated that the rate of rabbit bone tissue regeneration with the hybrid polymer was comparable to that with the osteoplastic matrix. Therefore, the hybrid polymer has good potential for use in further research and improvement in biomedical applications.
“…The brittle nature of HAp has been overcome through combination with several types of polymers 140 . An animal study identified that combining HAp with polymers improved the resorption rate of Hap 141 . Meanwhile, adding HAp onto several types of scaffolds has improved their bone regeneration potential 142–144 .…”
Section: Classificationmentioning
confidence: 99%
“…140 An animal study identified that combining HAp with polymers improved the resorption rate of Hap. 141 Meanwhile, adding HAp onto several types of scaffolds has improved their bone regeneration potential. [142][143][144] Polymer/HAp composites have produced resorbable membranes for GBR applications.…”
Over the past few decades, the field of biomaterials concerning bone tissue engineering has gained a significant amount of interest. This has led to new biomaterials to be used as bone substitute materials. Despite the rapid increase in the types and forms of bone substitutes, a comprehensive classification encompassing all types of bone graft materials that have so far been developed and evaluated is lacking. Therefore, this review aims to integrate and bring together the published data on bone substitutes within the last 5 years and produce a novel classification that would provide bone material researchers with a better understanding of what materials have so far been used and evaluated and the areas, where research is lacking and deserves more attention. The literature available in all major databases was obtained and filtered using an elimination criterion to extract all the articles related to studies that tested bone substitute materials in nonclinical and clinical trials over the last 5 years. The review article would provide bone material researchers with an insight into the materials that have been evaluated for bone tissue engineering applications and identify future perspectives for bone graft material research.
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