Cephalosporin acetylesterase activity of Fusaria

Singh, Kartar; Sun, Sheila; Rakhit, Suman

doi:10.1007/bf00499996

Cited by 6 publications

(2 citation statements)

References 7 publications

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“…4). For a long time, there has existed doubt about the occurrence of microbial acylases capable of hydrolysing cephalosporin C specifially into 7-aminocephalosporanic acid (7-ACA) and D-ex-aminoadipic acid (Demain et al 1963, Singh et al 1980, Walton 1964a. Enzymic hydrolysis to yield 7-ACA has also recently been claimed ( Figure 4).…”

Section: Bioconversion Of Cephalosporins With Immobilised Catalysmentioning

confidence: 99%

“…Indeed, the antibiotic properties of cephalosporins are determined not only by the side chain at the carbon-7 position, but also by the substituent at the carbon-3 position. Such enzymes that hydrolyse acetate from the C-3 carbon of cephalosporins have been reported to occur in mammalian tissue (O'Callaghan et al 1963), plant tissue (Jeffrey et al 1961;Wick et al 1971), in bacteria, actinomycetes and yeasts (Nishida et al 1968;Konecny et al 1980) and in fungi (Singh et al 1980): Among a large number of fungi tested, only Fusarium oxysporum strains were found to produce cephalosporin acetylesterase (Singh et al 1980). Acetate can be chemically removed but yields are low, due to unwanted side reactions such as lactone formation and double bond migration.…”

Section: B) Cephalosporin Acetylesterase Bioconversionsmentioning

confidence: 99%

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Enzyme Technology

1983

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Section: Bioconversion Of Cephalosporins With Immobilised Catalysmentioning

confidence: 99%

Section: B) Cephalosporin Acetylesterase Bioconversionsmentioning

confidence: 99%

Enzyme Technology

1983

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Use of microbial enzyme and cell preparations to synthesise oligopeptide antibiotics

Vandamme¹

1981

J. Chem. Technol. Biotechnol.

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Use of microbial enzyme and cell preparations to synthesise oligopeptide antibiotics

Vandamme

1981

J. Chem. Technol. Biotechnol.

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.Microbial enzymes and cells (iqfree or immobilised form) have so far been applied mainly in hydrolysis and bioconversion reactions, but recently there has been an increasing interest in the use of swh 'controlled catalytic biomass' to produce fermentation products and to apply the principle to organic synthesis. Particular attention is given here to the use of immobilised enzymes and cells for producing known or new peptide antibiotics. An evaluation is presented of the applicability of this concept in the fascinating field of peptide antibiotic bioconversions and fermentations. As an important example of the use of enzymes or cells to convert peptide antibiotics into useful derivatives, free and immobilised penicillin acylases, producing the penicillin nucleus 6-amino-penicillanic acid, are reviewed as well as their potential to synthesise semi-synthetic penicillins and cepbalosporins. The action of acetylesterase and a-amino acid ester hydrolase on cephalosp6rin compounds is also discussed. Use of enzymes and cells to synthesise antibiotics as gn alternative method to conventional fermentation is subsequently reviewed. The concept of total enzymic synthesis in vitro of antibiotics is illustrated in the exploitation of the 'multi-enzyme thiotemplate mechanism' of Bacillus brevis, to produce gramicidin S. Total synthesis of peptide antibiotics with immobilised living cells or mycelium as catalysts has been demonstrated recently in the cases of penicillin and bacitracin. These examples of complex multi-step enzyme reactions might indicate, the vast potential of immobilised enzyme and cell technology in fermentation, in biotechnological processes and in organic synthesis.

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Cephalosporin acetylesterase activity of Fusaria

Cited by 6 publications

References 7 publications

Enzyme Technology

Enzyme Technology

Use of microbial enzyme and cell preparations to synthesise oligopeptide antibiotics

Use of microbial enzyme and cell preparations to synthesise oligopeptide antibiotics

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