Centromere spreading in a case of megaloblastic anemia “cured” under TC 199 culture conditions

Mitotic disturbance associated with mosaic aneuploidies

“…PCD with separation mainly of the centromeric region without total chromatid repulsion has been observed in hematological and neoplastic disorders (Heath 1966;Gallo et al 1984;Littlefield et al 1986;Bamezai et al 1986;Zhang 1986). Similar observations restricted to chromosomes 1, 9, 16, and the Y-chromosome were made in Roberts' Syndrome or SC phocomelia (Freeman et al 1974;German 1979;Tomkins et al 1979;Petrinelli et al 1984;Parry et al 1986;R6mke et al 1987;Stanley et al 1988).…”

Premature Centromere Division: A cytogenetic study.

“…Thus, the results show principally two things: on the one hand, that the conditions of the culture medium affect the appearance of the PCD, which has been already suggested in the works of Fuster et al (1992) and Bamezai et al (1986); and on the other hand, that there are two factors which are most variable in the two culture mediums (thymidine and folic acid). The latter is the most influential factor in the appearance of this phenomenon.…”

“…The latter is the most influential factor in the appearance of this phenomenon. Nevertheless, in the work of Fuster et al (1992) the phenomenon was observed in deficient conditions of thymidine and folic acid, and in the study of Bamezai et al (1986) with deficient conditions of thymidine.…”

“…Previous reports neither solve the biologic implications of the phenomemon, as PCD has been described in patients with Robert's syndrome (German 1979, Tomkins et al 1979, Petrineli et al 1984, patients with Alzheimer disease (Moorhead and Heyman 1983) in chronic myeloid leukemia (Vig 1984), in cells of patients with leukemia treated with ara-C (Sandberg 1980), patients with acute non lymphocytic leukemia (Siraishi et al 1982), in megaloblastic anemia (Bamezai et al 1986), in Burkitt's lymphoma and nasopharyngeal carcinoma cell lines (Zhang 1986), in case of bladder carcinoma (Berrozpe et al 1990), and it has been relationated with aneuploidies (Fitzgerald et al 1986). However, PCD has also been described in healthy individuals (Rudd et al 1983, Gabarron et al 1986, Madan et al 1987, Fuster et al 1992).…”

“…According to Bamezai et al (1986), the alterations in the separation of the centromeres can appear due to errors in the pathways of DNA synthesis and also because of the replication inhibitor substances. Nevertheless, in our case low levels of folic acid have a more marked effect in the induction of PCD than a deficiency of thymidine.…”