Centrifugo-magnetophoretic particle separation

Abstract

There has been a recent surge of research output on magnetophoretic lab-on-a-chip systems due to their prospective use in a range of applications in the life sciences and clinical diagnostics. Manifold applications for batchmode or continuous-flow magnetophoretic separations of cells, proteins, and nucleic acids are found in bioanalytics, cell biology, and clinical diagnostics. To ensure stable hydrodynamic conditions and thus reproducible separation, state-of-the-art magnetophoretic lab-on-a-chip systems have been based on pressure-driven flow (Gijs in Microfluid

Rotational separation of non-spherical bioparticles using I-shaped pillar arrays in a microfluidic device

“…Bioparticles are separated in microfluidic devices by three general principles, namely size discrimination in sieving techniques, hydrodynamic laminar flow separation and non-inertia force fields, such as dielectrophoresis, acoustic, radiation and magnetic field [5][6][7][8] . The main separation criteria for all these methods depend on the spherical diameter of these bioparticles (that is, particles are considered as spherical) 5,9 .…”

Cluster size distribution of cancer cells in blood using stopped-flow centrifugation along scale-matched gaps of a radially inclined rail

“…Standard protocols for disc fabrication, polydimethylsiloxane priming, blood processing and cell culture were used and have been described elsewhere [33][34][35][36][37] . These protocols are also briefly outlined in the Supplementary Information.…”

CD4 cell isolation from blood using finger-actuated on-chip magnetophoresis for rapid HIV/AIDS diagnostics

“…Microchannels (40 μm deep) are fabr [4] and adhered to a glass base. A perm placed perpendicular to the pneumatically liquid (Fig.…”

“…The microfluidic chips used in from polydimethylsiloxane (PDM mixed at a ratio of 10:1 base procedures for making a master a rotating magnet in the PDMS have elsewhere [4][5]. Loading holes an the PDMS at appropriate locations that the chamber always t background of nonthe laminar flow towards , CD4-positive cells are w-free capture structure maining CD4 cells to the ssed to drive the sample releasing P1 the cycle reps a second time, a dualpproximately 30 seconds.…”