Rotational separation of non-spherical bioparticles using I-shaped pillar arrays in a microfluidic device

Zeming, Kerwin Kwek; Ranjan, Shashi; Zhang, Yong

doi:10.1038/ncomms2653

Cited by 157 publications

(161 citation statements)

References 28 publications

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“…Contrary to other sorting devices, 7,8,46 the sorting occurs over short length ($10 lm) and time scales (1 ms) where the flow is the fastest. Future work will focus on quantifying and theoretically describing the detailed hydrodynamic forces acting on particles and leading to their migration, advancing the work toward a more general understanding of particle dynamics in microfluidic flows as well as towards the development of improved devices for particle manipulation.…”

Section: Discussionmentioning

confidence: 97%

Particle migration and sorting in microbubble streaming flows

Thameem

Hilgenfeldt

2016

Biomicrofluidics

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Ultrasonic driving of semicylindrical microbubbles generates strong streaming flows that are robust over a wide range of driving frequencies. We show that in microchannels, these streaming flow patterns can be combined with Poiseuille flows to achieve two distinctive, highly tunable methods for size-sensitive sorting and trapping of particles much smaller than the bubble itself. This method allows higher throughput than typical passive sorting techniques, since it does not require the inclusion of device features on the order of the particle size. We propose a simple mechanism, based on channel and flow geometry, which reliably describes and predicts the sorting behavior observed in experiment. It is also shown that an asymptotic theory that incorporates the device geometry and superimposed channel flow accurately models key flow features such as peak speeds and particle trajectories, provided it is appropriately modified to account for 3D effects caused by the axial confinement of the bubble. V C 2016 AIP Publishing LLC.

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Section: Discussionmentioning

confidence: 97%

Particle migration and sorting in microbubble streaming flows

Thameem

Hilgenfeldt

2016

Biomicrofluidics

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“…Moreover, if the self-steered position of an object depends on a certain property of the object, a heterogeneous suspension can be separated by that property. For instance, both the stiffness 8 and shape 9 of blood components are of interest for microfluidic separations. From a theoretical perspective, a unifying framework for non-equilibrium self-organization and self-steering is highly sought after 10 .…”

mentioning

confidence: 99%

Engineering particle trajectories in microfluidic flows using particle shape

2013

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Recent advances in microfluidic technologies have created a demand for techniques to control the motion of flowing microparticles. Here we consider how the shape and geometric confinement of a rigid microparticle can be tailored for 'self-steering' under external flow. We find that an asymmetric particle, weakly confined in one direction and strongly confined in another, will align with the flow and focus to the channel centreline. Experimentally and theoretically, we isolate three viscous hydrodynamic mechanisms that contribute to particle dynamics. Through their combined effects, a particle is stably attracted to the channel centreline, effectively behaving as a damped oscillator. We demonstrate the use of self-steering particles for microfluidic device applications, eliminating the need for external forces or sheath flows.

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“…However, pore-based filtration may be ineffective with deformable bio-particles and/or bio-particles with unique shapes. By introducing series of posts, a size dependent lateral displacement of bio-particles can also be achieved, which is known as deterministic lateral displacement (DLD) (see Fig 1a) [16][17][18][19][20]. DLD can be utilized for bio-particle separation, sorting and focusing.…”

Section: Hydrodynamic-based (Hd)mentioning

confidence: 99%

“…Separation and sorting of spherical and non-spherical particles is important for clinical applications such as separation of yeast cells at different cell stage and separation of parasites form blood. More recently, some research efforts have been focused on the implementation of HD applications on separation by shape using DLD [19,18,20], PFF [28] and inertial microfluidics [40].…”

Section: Hydrodynamic-based (Hd)mentioning

confidence: 99%

“…HD methods mainly use the shape and the size of the bioparticles to manipulate them; therefore, mainly HD devices have been proposed for separation and sorting of (bio)particles by size [16-18,21-27,29-31,33-35,37-39,114,115] and shape [19,18,20,28,40]. Separation by size includes (i) enrichment of low-concentrated cancer cells from the peritoneal wash [114], separation of plasma from whole blood [115] using filtration, (ii) separation of white blood cells (WBCs) and RBCs [17], separation of plasma from whole blood [17], separation of parasites from human blood [19], capturing of circulating tumor cells (assisted by affinity-based cell culture) using DLD, (iii) separation of RBCs and WBCs [22] and separation of U937 (human leukemic monocyte lymphoma cell line) cells at different phase using hydrophoresis, (iv) enrichment of leukocyte [30] using PFF, and (v) separation of Escherichia coli from RBCs, separation of WBCs, MCF-7 and MDA-MB-231 breast cancer cells [116], focusing of Jurkat and MCF7 (a breast carcinoma cell line) [38], separation of HeLa and MCF cells [34], focusing of HeLa and Jurkat cells [39], separation of plasma from whole blood [117] using inertial microfluidics.…”

Section: Applicationmentioning

confidence: 99%

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Microfluidic bio-particle manipulation for biotechnology

Çetin

Özer

Solmaz

2014

Biochemical Engineering Journal

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a b s t r a c tMicrofluidics and lab-on-a-chip technology offers unique advantages for the next generation devices for diagnostic therapeutic applications. For chemical, biological and biomedical analysis in microfluidic systems, there are some fundamental operations such as separation, focusing, filtering, concentration, trapping, detection, sorting, counting, washing, lysis of bio-particles, and PCR-like reactions. The combination of these operations led to the complete analysis systems for specific applications. Manipulation of the bio-particles is the key ingredient for these applications. Therefore, microfluidic bio-particle manipulation has attracted a significant attention from the academic community. Considering the size of the bio-particles and the throughput of the practical applications, manipulation of the bio-particles is a challenging problem. Different techniques are available for the manipulation of bio-particles in microfluidic systems. In this review, some of the techniques for the manipulation of bio-particles; namely hydrodynamic based, electrokinetic-based, acoustic-based, magnetic-based and optical-based methods have been discussed. The comparison of different techniques and the recent applications regarding the microfluidic bio-particle manipulation for different biotechnology applications are presented. Finally, challenges and the future research directions for microfluidic bio-particle manipulation are addressed.

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Rotational separation of non-spherical bioparticles using I-shaped pillar arrays in a microfluidic device

Cited by 157 publications

References 28 publications

Particle migration and sorting in microbubble streaming flows

Particle migration and sorting in microbubble streaming flows

Engineering particle trajectories in microfluidic flows using particle shape

Microfluidic bio-particle manipulation for biotechnology

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